FastQCFastQC Report
Thu 26 May 2016
SRR2132760_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132760_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2288004
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1289885.637577556682593No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320751.4018769197955947No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT105200.45978940596257695No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44650.19514826023031429No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG43140.18854862141849402No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37190.1625434221268844No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36860.16110111695608922No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA31100.13592633579311925No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29590.12932669698129898No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26360.1152095887944252No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC23170.10126730547673868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT26350.085.7406541
AACGAGT308500.083.04421
ACGAGTG343150.081.4153752
CGAGTGG356850.080.42033
GTGGAAT383600.079.33946
TGGAATC387600.078.3682567
GAGTGGA377800.078.28584
GGAATCA388350.078.190998
GAATCAC389550.078.0345849
AGTGGAA381000.077.418535
ATCGAGT8650.070.193961
AACGTGT12400.069.4633261
ACGTGTG14200.065.879372
CGTGTGG15550.062.877993
TCGAGTG9900.062.2043572
AGTACTT218550.040.82630520-21
ATGAGTG22050.040.720162
GAGTACT215400.040.5178620-21
ATCACTG397250.039.89958210-11
TGAGTAC395900.039.75666418-19