##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132760_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2288004
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.725451965993066	31.0	23.0	33.0	16.0	34.0
2	28.02479060351293	31.0	25.0	33.0	16.0	34.0
3	29.109372623474435	31.0	28.0	34.0	16.0	34.0
4	34.65679911398756	35.0	35.0	37.0	32.0	37.0
5	33.763398140912344	35.0	35.0	37.0	28.0	37.0
6	35.19663033805885	37.0	35.0	37.0	32.0	37.0
7	35.61601509437921	37.0	35.0	37.0	33.0	37.0
8	35.882428964285026	37.0	35.0	37.0	35.0	37.0
9	37.845934709904355	39.0	38.0	39.0	35.0	39.0
10-11	37.8454989589179	39.0	38.0	39.0	35.0	39.0
12-13	37.82246643799574	39.0	38.0	39.0	35.0	39.0
14-15	39.228993043718454	41.0	39.0	41.0	36.0	41.0
16-17	39.23435907454707	40.0	39.0	41.0	36.0	41.0
18-19	39.168512380223106	40.0	39.0	41.0	36.0	41.0
20-21	39.14321762549366	40.0	39.0	41.0	36.0	41.0
22-23	39.042649182431504	40.0	39.0	41.0	35.5	41.0
24-25	38.94434537701857	40.0	39.0	41.0	35.0	41.0
26-27	38.77806660303041	40.0	39.0	41.0	35.0	41.0
28-29	38.615704561705314	40.0	38.5	41.0	35.0	41.0
30-31	38.50416039482448	40.0	38.0	41.0	34.5	41.0
32-33	38.35343010763967	40.0	38.0	41.0	34.0	41.0
34-35	38.270673040781396	40.0	38.0	41.0	34.0	41.0
36-37	38.134914099800525	40.0	38.0	41.0	34.0	41.0
38-39	37.73629460438006	40.0	36.5	41.0	33.5	41.0
40-41	37.511472444978246	40.0	36.0	41.0	33.0	41.0
42-43	37.36577602137059	40.0	35.0	41.0	33.0	41.0
44-45	37.2310505138977	40.0	35.0	41.0	33.0	41.0
46-47	37.102190817848225	40.0	35.0	41.0	33.0	41.0
48-49	36.95420528110965	40.0	35.0	41.0	32.5	41.0
50-51	36.708039409022014	39.5	35.0	41.0	31.5	41.0
52-53	36.37268968935369	39.0	35.0	41.0	31.0	41.0
54-55	35.68711899105072	39.0	35.0	41.0	28.5	41.0
56-57	35.08492926585792	39.0	35.0	41.0	25.5	41.0
58-59	34.76895363819294	39.0	35.0	41.0	24.0	41.0
60-61	34.52494685323977	38.5	35.0	40.0	23.5	41.0
62-63	34.25487848797467	38.0	35.0	40.0	23.0	41.0
64-65	33.982258335212705	37.0	34.0	40.0	22.0	41.0
66-67	33.67997630248898	37.0	34.0	40.0	22.0	41.0
68-69	33.36195216441929	36.0	34.0	39.0	20.5	41.0
70-71	33.02823640168461	36.0	34.0	39.0	20.0	41.0
72-73	32.69896883921532	35.0	34.0	39.0	18.5	40.0
74-75	32.36280290593898	35.0	33.5	37.5	17.5	40.0
76-77	32.03740705872892	35.0	33.0	37.0	14.0	39.0
78-79	31.70622407128659	35.0	33.0	36.5	9.0	39.0
80-81	31.408391768545858	35.0	33.0	36.0	6.0	39.0
82-83	31.125518574268227	35.0	33.0	36.0	2.0	37.0
84-85	30.860649937674935	35.0	33.0	35.0	2.0	37.0
86-87	30.652702967302503	35.0	33.0	35.0	2.0	36.5
88-89	30.453275649867745	35.0	33.0	35.0	2.0	36.0
90-91	30.274592832879662	35.0	33.0	35.0	2.0	36.0
92-93	30.104492605782156	35.0	32.0	35.0	2.0	36.0
94-95	29.937934986127647	35.0	32.0	35.0	2.0	35.5
96-97	29.777802398946854	35.0	32.0	35.0	2.0	35.0
98-99	29.623631558336434	35.0	32.0	35.0	2.0	35.0
100	29.52263326462716	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.18631125801983117
1101	2	2.3596829963442936
1101	3	1.1137742825135497
1101	4	0.6454742410586292
1101	5	2.2875934840728647
1101	6	1.0503491104383542
1101	7	0.5839240310611871
1101	8	0.41552824178848624
1101	9	0.3235593238693326
1101	10-11	0.4670146580406467
1101	12-13	0.435922057441239
1101	14-15	0.5208477231892132
1101	16-17	0.5103456846565209
1101	18-19	0.42972159127096177
1101	20-21	0.4451752019197528
1101	22-23	0.4154138015210478
1101	24-25	0.4101981261488703
1101	26-27	0.30789553762562605
1101	28-29	0.3732535599235334
1101	30-31	0.3657555762887128
1101	32-33	0.4068461349722057
1101	34-35	0.393662286630196
1101	36-37	0.43046391062193834
1101	38-39	0.47292321293355144
1101	40-41	0.48323658916336143
1101	42-43	0.5457682932540493
1101	44-45	0.5838194929677201
1101	46-47	0.5371164860462727
1101	48-49	0.5995341867460837
1101	50-51	0.6967544854854779
1101	52-53	0.8658631378985575
1101	54-55	0.8566421542423583
1101	56-57	0.7506487200173098
1101	58-59	0.7370713553331001
1101	60-61	0.8054088098745922
1101	62-63	0.8302896956212891
1101	64-65	0.8472825613068125
1101	66-67	0.8149936459236145
1101	68-69	0.8967935642063765
1101	70-71	0.7516158341885344
1101	72-73	0.7711836198485145
1101	74-75	0.813729103049198
1101	76-77	0.742010056766528
1101	78-79	0.73093784382921
1101	80-81	0.7679106257226032
1101	82-83	0.838426649262626
1101	84-85	0.8221750609052023
1101	86-87	0.8187922981076063
1101	88-89	0.8027483949142322
1101	90-91	0.7706028047358267
1101	92-93	0.8986809347281728
1101	94-95	0.8812539626680405
1101	96-97	0.8364538877713983
1101	98-99	0.9750573405842715
1101	100	0.9357730577820647
1102	1	1.4562930254509112
1102	2	2.9527598949677234
1102	3	3.23614819899651
1102	4	1.4774745235073112
1102	5	2.6832465594439014
1102	6	1.2315496467532654
1102	7	0.7275021354366658
1102	8	0.49659931307920857
1102	9	0.4473453431096033
1102	10-11	0.5660989011738309
1102	12-13	0.5322174363106953
1102	14-15	0.6952278200657318
1102	16-17	0.6492721771052388
1102	18-19	0.5275160369907326
1102	20-21	0.4683478447391991
1102	22-23	0.41676123506402263
1102	24-25	0.4990867524692675
1102	26-27	0.5576002535621498
1102	28-29	0.5949225362552326
1102	30-31	0.6113065523683545
1102	32-33	0.6451049274534455
1102	34-35	0.6963898505271757
1102	36-37	0.6110119173331867
1102	38-39	0.6027243482422193
1102	40-41	0.6786705417457384
1102	42-43	0.6598061756991385
1102	44-45	0.760469869603476
1102	46-47	0.765944896501594
1102	48-49	0.6995856196949006
1102	50-51	0.7203431352682443
1102	52-53	0.7236707973779701
1102	54-55	0.7504301961978044
1102	56-57	0.559825165485023
1102	58-59	0.5911609393908392
1102	60-61	0.5480983050904058
1102	62-63	0.53709445831376
1102	64-65	0.5491898705948728
1102	66-67	0.6081797402209617
1102	68-69	0.6201989388334823
1102	70-71	0.5729605009159684
1102	72-73	0.5063741236086869
1102	74-75	0.5688163090803187
1102	76-77	0.5866595531413985
1102	78-79	0.5625501461573279
1102	80-81	0.5132370705714031
1102	82-83	0.5620638343632898
1102	84-85	0.4605160842134062
1102	86-87	0.49320446585061717
1102	88-89	0.47622702615215573
1102	90-91	0.492069755707913
1102	92-93	0.4796237225529474
1102	94-95	0.4856619696381088
1102	96-97	0.5406557057956753
1102	98-99	0.6200167076404028
1102	100	0.5665282310378856
1103	1	1.2064257095090554
1103	2	2.937027120761922
1103	3	3.2119227689333627
1103	4	1.4172223810009612
1103	5	2.604733316412336
1103	6	1.2570973682301627
1103	7	0.6720179924536964
1103	8	0.5001078334007474
1103	9	0.4080362882237907
1103	10-11	0.47870066184326276
1103	12-13	0.4826577249799868
1103	14-15	0.637503734298484
1103	16-17	0.5129108750619977
1103	18-19	0.49701929281321355
1103	20-21	0.5127385558374655
1103	22-23	0.5282971560672891
1103	24-25	0.5439219341013626
1103	26-27	0.6448220525807287
1103	28-29	0.6895983992946881
1103	30-31	0.6305208888648295
1103	32-33	0.7293596582003374
1103	34-35	0.7248112306988332
1103	36-37	0.6835987068601881
1103	38-39	0.5991296970120814
1103	40-41	0.6789551174590187
1103	42-43	0.6347255021069103
1103	44-45	0.7196496149026501
1103	46-47	0.6813640072788161
1103	48-49	0.8246809584559216
1103	50-51	0.838112730235288
1103	52-53	0.7632695163053924
1103	54-55	0.8954617738242305
1103	56-57	0.891383099068392
1103	58-59	0.8649748719616639
1103	60-61	0.9178153650556524
1103	62-63	0.8814481560356526
1103	64-65	0.951915961028682
1103	66-67	0.9136562507114476
1103	68-69	0.8967719584678946
1103	70-71	0.8623172546676301
1103	72-73	0.884135965446049
1103	74-75	0.8604448834877587
1103	76-77	0.8530218142165857
1103	78-79	0.7996487570841353
1103	80-81	0.8049158543113215
1103	82-83	0.8328386196274096
1103	84-85	0.8570602264051566
1103	86-87	0.7761393785830926
1103	88-89	0.7750262682810352
1103	90-91	0.8586886639764053
1103	92-93	0.8914322094667426
1103	94-95	0.9282123166250358
1103	96-97	0.9072529465468655
1103	98-99	0.9318470912147703
1103	100	0.9522562720874213
1104	1	6.21846926667784
1104	2	5.26737888379018
1104	3	4.206899823639091
1104	4	1.9319731588075442
1104	5	2.894188722168103
1104	6	1.32705582039506
1104	7	0.7055181869053442
1104	8	0.5138356010958418
1104	9	0.38084287365288105
1104	10-11	0.45809799137398244
1104	12-13	0.39728677605595664
1104	14-15	0.541672939639426
1104	16-17	0.39971797902881434
1104	18-19	0.39329680772117115
1104	20-21	0.4205951153396654
1104	22-23	0.4281659660232151
1104	24-25	0.387016307967059
1104	26-27	0.3882981350282293
1104	28-29	0.37402791057288454
1104	30-31	0.4805261390592861
1104	32-33	0.5502795765306487
1104	34-35	0.533891182734088
1104	36-37	0.5326116378946679
1104	38-39	0.561267368777699
1104	40-41	0.5708063943581649
1104	42-43	0.6431903711761251
1104	44-45	0.6579726314958592
1104	46-47	0.5307209232757089
1104	48-49	0.6958577798196757
1104	50-51	0.7493259140569108
1104	52-53	0.6113966876821095
1104	54-55	0.5992346867098988
1104	56-57	0.596470148588736
1104	58-59	0.709944731956476
1104	60-61	0.7118433336841221
1104	62-63	0.6961906696472653
1104	64-65	0.6628929509172039
1104	66-67	0.7370948363997982
1104	68-69	0.6151626118254256
1104	70-71	0.626485422933122
1104	72-73	0.6276165202814212
1104	74-75	0.6680824580275555
1104	76-77	0.6463336498401269
1104	78-79	0.6304843806257523
1104	80-81	0.6424939590559404
1104	82-83	0.6396485107344887
1104	84-85	0.6408417275718676
1104	86-87	0.6997003067656173
1104	88-89	0.6394697152605531
1104	90-91	0.6612326748656976
1104	92-93	0.7101571252043613
1104	94-95	0.7217058024948813
1104	96-97	0.6782114635289709
1104	98-99	0.6739956522725876
1104	100	0.7368336638426705
1105	1	1.4801424960309468
1105	2	3.1501108577098975
1105	3	3.305388829008564
1105	4	1.4742753193802898
1105	5	2.6458945518791737
1105	6	1.2335543501828425
1105	7	0.756595724619487
1105	8	0.4466906253863101
1105	9	0.3661036765166017
1105	10-11	0.46369336340160316
1105	12-13	0.4166021046164232
1105	14-15	0.5237096629838405
1105	16-17	0.4032861201691915
1105	18-19	0.4459752467393301
1105	20-21	0.42819847765109387
1105	22-23	0.4007658126097695
1105	24-25	0.40600250075662814
1105	26-27	0.431610139630628
1105	28-29	0.46880510949898735
1105	30-31	0.4813658859270902
1105	32-33	0.48977666806734277
1105	34-35	0.4848010203715276
1105	36-37	0.5273991283063211
1105	38-39	0.4484586242110993
1105	40-41	0.5198939359076391
1105	42-43	0.44200729779336
1105	44-45	0.4803778667298175
1105	46-47	0.5554712748481947
1105	48-49	0.5148639163576476
1105	50-51	0.5281720531517706
1105	52-53	0.5383694567948609
1105	54-55	0.5274374801131145
1105	56-57	0.3824130660011633
1105	58-59	0.337374737070256
1105	60-61	0.36949735439493026
1105	62-63	0.41541257580936986
1105	64-65	0.40511239607189253
1105	66-67	0.4418352160980916
1105	68-69	0.4263226092685386
1105	70-71	0.5316957591642648
1105	72-73	0.47302600634636605
1105	74-75	0.5436035843379585
1105	76-77	0.47783806047679533
1105	78-79	0.4989172622416973
1105	80-81	0.49869665657575624
1105	82-83	0.48582282676619215
1105	84-85	0.47683233099809996
1105	86-87	0.48793515718750413
1105	88-89	0.5172394352810095
1105	90-91	0.4864482846841618
1105	92-93	0.5383069590064586
1105	94-95	0.6002277597948051
1105	96-97	0.5940383620204095
1105	98-99	0.5584392728299861
1105	100	0.5748175554304993
1106	1	-0.7156746438230499
1106	2	1.8874003050970742
1106	3	2.7583037137992754
1106	4	1.2121870540529045
1106	5	2.4927304206171286
1106	6	1.1550744448547619
1106	7	0.6108430661323965
1106	8	0.42214681391048714
1106	9	0.33407577474619643
1106	10-11	0.43708362574977855
1106	12-13	0.3955264785731174
1106	14-15	0.4063085549458094
1106	16-17	0.3405684748335389
1106	18-19	0.2797450978304994
1106	20-21	0.3503078664202661
1106	22-23	0.3058964516575955
1106	24-25	0.36750575622538406
1106	26-27	0.3729993173318107
1106	28-29	0.4059363456097529
1106	30-31	0.44625274724641173
1106	32-33	0.368737337781603
1106	34-35	0.43433616747856263
1106	36-37	0.45744424023384767
1106	38-39	0.5874344168326218
1106	40-41	0.5566109010960218
1106	42-43	0.5907161784479342
1106	44-45	0.6116735277582492
1106	46-47	0.5993379776870995
1106	48-49	0.668397571327425
1106	50-51	0.7501272743416791
1106	52-53	0.8113131636546456
1106	54-55	0.7515556567073531
1106	56-57	0.7258925360792006
1106	58-59	0.7896259549414779
1106	60-61	0.6948028111822069
1106	62-63	0.674643663545055
1106	64-65	0.6498013541717143
1106	66-67	0.6508741079482476
1106	68-69	0.6628845162241106
1106	70-71	0.6382087575377469
1106	72-73	0.665371287634585
1106	74-75	0.6342315490522168
1106	76-77	0.5941575120318383
1106	78-79	0.5985807447243943
1106	80-81	0.637301040164509
1106	82-83	0.6450421669765412
1106	84-85	0.5992774636511982
1106	86-87	0.6450752114185541
1106	88-89	0.7127209547723758
1106	90-91	0.7663444851886076
1106	92-93	0.7093897720388433
1106	94-95	0.7531889260646736
1106	96-97	0.8329780539179872
1106	98-99	0.8430719299232337
1106	100	0.7973637935146769
1107	1	-1.059190663260182
1107	2	1.604192343774013
1107	3	-0.7192412434587254
1107	4	-0.07969653938700816
1107	5	1.8935477898048632
1107	6	0.8257531391770598
1107	7	0.3690628514162597
1107	8	0.2969657546932609
1107	9	0.296594692569613
1107	10-11	0.2974090935027931
1107	12-13	0.28386512421009513
1107	14-15	0.37146831150684534
1107	16-17	0.24893786585177224
1107	18-19	0.2192737344255704
1107	20-21	0.1865306448465418
1107	22-23	0.1610514416362534
1107	24-25	0.030981546447250707
1107	26-27	0.022828787143559737
1107	28-29	0.10771118327566143
1107	30-31	0.10486843224170883
1107	32-33	0.06819876947055548
1107	34-35	0.14601281436976876
1107	36-37	0.1696606475955562
1107	38-39	0.14475025748694748
1107	40-41	0.223287228365173
1107	42-43	0.2124636128899553
1107	44-45	0.3022199704880464
1107	46-47	0.3078224120927331
1107	48-49	0.16635980083827206
1107	50-51	0.2081287300094843
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2216	4	-0.9288180396943275
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2216	80-81	0.10931199900599964
2216	82-83	0.14378115742612252
2216	84-85	0.20184890609871076
2216	86-87	0.22469593722754055
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2216	90-91	0.1933622004587079
2216	92-93	0.07377010897464942
2216	94-95	-0.003255964046939397
2216	96-97	-0.002426489030330714
2216	98-99	0.01865108298419571
2216	100	0.03396849530334478
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1124.0
3	266.0
4	261.0
5	580.0
6	1401.0
7	2433.0
8	2992.0
9	4156.0
10	3501.0
11	4080.0
12	3789.0
13	5164.0
14	5172.0
15	5695.0
16	6888.0
17	8059.0
18	11187.0
19	19346.0
20	38033.0
21	44920.0
22	21323.0
23	14007.0
24	13830.0
25	15051.0
26	16223.0
27	18381.0
28	21556.0
29	25736.0
30	31037.0
31	38391.0
32	50008.0
33	69861.0
34	103762.0
35	187434.0
36	295538.0
37	499310.0
38	549520.0
39	147623.0
40	366.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.618472524577191	38.440732444519135	25.783839757847588	21.15695527305608
2	16.09803153667419	37.55915967473762	25.69877038790564	20.644038400682554
3	19.960121192413062	25.591975635639518	19.35044398172673	35.09745919022069
4	30.159693776759134	27.080066293590395	22.089559283987263	20.670680645663207
5	18.49935203861981	35.80365786764979	22.340790534737504	23.356199558992895
6	34.42183037979885	23.495843662070953	24.126867423376467	17.955458534753735
7	20.503439115397544	22.817482778198585	36.63386718987318	20.0452109165307
8	33.43682965589221	23.14480219440176	23.870194282877126	19.548173866828904
9	33.005973765780126	25.073950919666228	23.615255917384758	18.304819397168888
10-11	18.287052819837726	39.1968720334405	23.708066069814564	18.808009076907208
12-13	18.180497062068074	24.88771872776446	30.653399207344044	26.278385002823423
14-15	18.37658500597027	31.792273964556006	23.64869117361683	26.182449855856895
16-17	25.80817166403555	24.5281913842808	30.77147155337141	18.89216539831224
18-19	18.62212653474382	24.631796972382915	30.95328941732619	25.792787075547068
20-21	25.876790425191565	31.964651285574675	23.629373025571635	18.529185263662125
22-23	25.497180074860008	24.89764003909084	30.98775176966474	18.617428116384414
24-25	18.52029104844222	31.89107186875547	23.593162424541216	25.995474658261088
26-27	18.828742282567934	27.57582816798237	34.87818192466615	18.717247624783546
28-29	25.036494691442847	24.64602334611303	31.690351065819815	18.627130896624305
30-31	23.62943858489758	25.338373534312	31.96452016692278	19.067667713867635
32-33	20.57210127255022	26.729760961956362	32.649724388593725	20.048413376899692
34-35	20.278766995162595	26.463611951727355	32.9665070515611	20.291114001548948
36-37	20.200139510245613	26.653690290751243	33.086830267779256	20.059339931223896
38-39	20.522582128352923	26.374844624397507	32.838731925293835	20.26384132195573
40-41	20.23438333149767	26.928624250656902	32.70621467444987	20.13077774339555
42-43	20.27240155901542	26.64071949851015	32.855539760465824	20.231339182008604
44-45	20.31954489590053	26.852269489039355	32.523151183302126	20.30503443175799
46-47	20.510431799944406	26.538874057912487	32.63851374385709	20.312180398286017
48-49	20.297866612121307	26.834087702643878	32.55197543360938	20.316070251625433
50-51	20.51735923538595	27.08161786430443	32.10641240137692	20.294610498932695
52-53	20.619068785372036	27.093060711074862	31.807743965146866	20.480126538406232
54-55	21.06449551661623	27.666560023496462	30.483338315842108	20.7856061440452
56-57	21.24235796790565	28.198333569346907	29.359673322249435	21.199635140498003
58-59	21.576011230749597	28.76229674423646	28.608450859351642	21.0532411656623
60-61	21.474132038230703	28.84551338196961	28.450256205845793	21.230098373953894
62-63	21.007708468913723	29.031168228363335	28.286479519476153	21.67464378324679
64-65	21.199394756302873	28.97031648546069	28.677900038636295	21.15238871960014
66-67	21.30283017486643	28.6516607164281	28.801922724331497	21.243586384373973
68-69	21.184709467291142	28.77894881302655	28.86164097615214	21.17470074353017
70-71	20.632918473918753	29.036465845339432	29.015071651972633	21.31554402876918
72-73	20.742261886290734	28.800831121423887	29.008392945258137	21.448514047027242
74-75	20.883061177349344	28.858994696449358	28.739654426767885	21.518289699433414
76-77	21.252055023829865	28.72638675728767	28.704664639123283	21.31689357975918
78-79	21.231479156545912	29.065887994146845	28.226332514057606	21.476300335249636
80-81	21.188663976812737	29.185301185914824	28.174365996563346	21.451668840709086
82-83	21.146803486223856	29.41030438680109	27.969992705346254	21.472899421628803
84-85	21.325182880377415	29.50708291320126	27.68363322786871	21.48410097855261
86-87	21.260260309916486	29.703319946844488	27.509617008890064	21.526802734348966
88-89	21.64694642142234	29.30912708194566	27.49997377626962	21.543952720362377
90-91	21.476292415828308	29.633868327038176	27.44654506420886	21.443294192924654
92-93	21.19163690273269	29.788627991909106	27.377946017576893	21.641789087781316
94-95	21.2712961321956	29.991977722062053	27.159683103631615	21.57704304211073
96-97	21.076540406159527	29.620388047385276	27.368367914236565	21.934703632218632
98-99	21.452647478920344	29.726204857717786	26.843803376591136	21.977344286770734
100	21.328677747066877	29.909606801386712	26.787627993657352	21.97408745788906
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	34039.0
1	18624.0
2	2333.5
3	1299.5
4	1095.5
5	976.5
6	977.0
7	1198.5
8	1221.5
9	1106.5
10	1108.0
11	1656.0
12	11984.5
13	13771.5
14	5313.0
15	5105.0
16	5864.5
17	6625.0
18	7403.0
19	8467.0
20	9656.5
21	11069.5
22	12744.5
23	14957.0
24	17274.0
25	19625.0
26	23233.5
27	26700.5
28	28963.0
29	31122.5
30	33644.0
31	36864.5
32	40254.5
33	43798.5
34	47986.5
35	52143.5
36	55389.0
37	57901.0
38	59868.0
39	61497.5
40	63500.0
41	65603.0
42	67351.5
43	68863.0
44	70203.0
45	71353.0
46	72406.5
47	74079.5
48	75334.5
49	75944.0
50	79961.0
51	79414.0
52	71947.0
53	67327.0
54	64491.5
55	61618.5
56	57020.5
57	51335.0
58	47159.5
59	40518.0
60	32989.5
61	27669.0
62	22674.5
63	18157.5
64	14343.0
65	11332.0
66	8938.0
67	7166.5
68	5833.0
69	4336.5
70	3016.5
71	2352.5
72	1936.5
73	1379.5
74	866.0
75	585.5
76	399.5
77	278.0
78	187.0
79	124.5
80	81.0
81	42.5
82	22.0
83	9.5
84	5.0
85	2.5
86	1.0
87	1.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13819905909255403
2	0.005594395813993332
3	0.0031468476453712494
4	0.0
5	0.0025786668205125514
6	0.012805921667969112
7	0.0026660792551061975
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	8.741243459364582E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	9.396836718816926E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	1.3111865189046872E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	4.370621729682291E-5
64-65	0.0
66-67	2.1853108648411454E-5
68-69	0.0
70-71	0.0
72-73	4.8076839026505195E-4
74-75	3.715028470229947E-4
76-77	5.900339335071093E-4
78-79	3.277966297261718E-4
80-81	0.0010270961064753384
82-83	0.0011800678670142186
84-85	0.0014423051707951559
86-87	2.1853108648411454E-5
88-89	0.0
90-91	8.741243459364582E-5
92-93	0.0
94-95	2.1853108648411454E-5
96-97	0.0015734238226856247
98-99	6.992994767491665E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2288004.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.66052487222153
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.4400254424599	69.94060844178028
2	5.305883466578065	8.028918559842774
3	0.9531766067569005	2.163535270894506
4	0.3513723754326586	1.063400734033369
5	0.192113123144541	0.726768986597885
6	0.12273938374849698	0.5571915718142579
7	0.08688970830486514	0.4601884655438227
8	0.06612929363823007	0.40027016528781983
9	0.062224309001365	0.42371314909691166
>10	0.3974115181000917	5.1416477323548895
>50	0.011404683941446607	0.5741936241426067
>100	0.009417779848886284	1.4473232418567528
>500	3.4637401274051225E-4	0.17689616734080083
>1k	6.927480254809993E-4	1.4009302892070787
>5k	0.0	0.0
>10k+	1.7318700637024983E-4	7.494413600206341
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	128988	5.637577556682593	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32075	1.4018769197955947	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10520	0.45978940596257695	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4465	0.19514826023031429	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	4314	0.18854862141849402	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3719	0.1625434221268844	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3686	0.16110111695608922	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	3110	0.13592633579311925	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2959	0.12932669698129898	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2636	0.1152095887944252	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	2317	0.10126730547673868	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.61536780530104E-4	0.0	0.0	0.0	0.0
2	9.61536780530104E-4	0.0	0.0	4.370621729682291E-5	0.0
3	9.61536780530104E-4	4.370621729682291E-5	0.0	4.370621729682291E-5	0.0
4	9.61536780530104E-4	4.370621729682291E-5	0.0	4.370621729682291E-5	0.0
5	9.61536780530104E-4	8.741243459364582E-5	0.0	4.370621729682291E-5	0.0
6	9.61536780530104E-4	8.741243459364582E-5	0.0	4.370621729682291E-5	0.0
7	0.001005242997826927	1.3111865189046872E-4	0.0	4.370621729682291E-5	0.0
8	0.001005242997826927	1.3111865189046872E-4	0.0	8.741243459364582E-5	0.0
9	0.001005242997826927	1.3111865189046872E-4	0.0	8.741243459364582E-5	0.0
10-11	0.001005242997826927	1.5297176053888017E-4	0.0	1.0926554324205726E-4	0.0
12-13	0.001005242997826927	1.7482486918729163E-4	0.0	1.3111865189046872E-4	0.0
14-15	0.00104894921512375	1.7482486918729163E-4	0.0	1.7482486918729163E-4	0.0
16-17	0.001114508541068984	1.7482486918729163E-4	0.0	2.6223730378093743E-4	0.0
18-19	0.001158214758365807	1.7482486918729163E-4	0.0	3.0594352107776035E-4	0.0
20-21	0.00120192097566263	2.1853108648411455E-4	0.0	3.277966297261718E-4	0.0
22-23	0.001245627192959453	2.1853108648411455E-4	0.0	3.715028470229947E-4	2.1853108648411454E-5
24-25	0.0012674803016078644	2.1853108648411455E-4	0.0	3.933559556714062E-4	4.370621729682291E-5
26-27	0.0013330396275530987	2.6223730378093743E-4	0.0	3.933559556714062E-4	4.370621729682291E-5
28-29	0.0013767458448499215	2.6223730378093743E-4	0.0	4.5891528161664055E-4	4.370621729682291E-5
30-31	0.0014860113880919789	2.840904124293489E-4	0.0	5.026214989134635E-4	4.370621729682291E-5
32-33	0.001638983148630859	3.0594352107776035E-4	0.0	5.900339335071093E-4	4.370621729682291E-5
34-35	0.0019886328870054423	3.0594352107776035E-4	0.0	6.992994767491665E-4	4.370621729682291E-5
36-37	0.0024694012772704943	4.1520906431981764E-4	0.0	7.430056940459894E-4	4.370621729682291E-5
38-39	0.003124994536722838	4.370621729682291E-4	0.0	7.430056940459894E-4	4.370621729682291E-5
40-41	0.004195796860494999	4.370621729682291E-4	0.0	7.867119113428124E-4	4.370621729682291E-5
42-43	0.005354011618860806	4.80768390265052E-4	0.0	7.867119113428124E-4	4.370621729682291E-5
44-45	0.007233378962624191	4.80768390265052E-4	0.0	7.867119113428124E-4	4.370621729682291E-5
46-47	0.008741243459364582	5.463277162102863E-4	0.0	8.304181286396353E-4	4.370621729682291E-5
48-49	0.010795435672315258	5.681808248586978E-4	0.0	8.304181286396353E-4	4.370621729682291E-5
50-51	0.013221130732288928	5.681808248586978E-4	0.0	8.304181286396353E-4	4.370621729682291E-5
52-53	0.017110984071706168	5.681808248586978E-4	0.0	8.304181286396353E-4	4.370621729682291E-5
54-55	0.021874961757059865	5.681808248586978E-4	0.0	8.959774545848696E-4	4.370621729682291E-5
56-57	0.027360092027811138	5.900339335071093E-4	0.0	0.001005242997826927	4.370621729682291E-5
58-59	0.03406899638287346	6.118870421555207E-4	0.0	0.001005242997826927	4.370621729682291E-5
60-61	0.04219835280008252	6.118870421555207E-4	0.0	0.001005242997826927	4.370621729682291E-5
62-63	0.052884522929155714	6.118870421555207E-4	0.0	0.001005242997826927	4.370621729682291E-5
64-65	0.06564673837982801	6.555932594523436E-4	0.0	0.001005242997826927	4.370621729682291E-5
66-67	0.08035388050020892	6.555932594523436E-4	0.0	0.0010270961064753384	4.370621729682291E-5
68-69	0.09838269513514836	6.555932594523436E-4	0.0	0.001114508541068984	4.370621729682291E-5
70-71	0.12301114858190808	6.774463681007551E-4	0.0	0.0011363616497173956	4.370621729682291E-5
72-73	0.1505023592616097	6.992994767491665E-4	0.0	0.0011800678670142186	4.370621729682291E-5
74-75	0.1860355139239267	7.430056940459894E-4	0.0	0.0012674803016078644	4.370621729682291E-5
76-77	0.2272504768348307	7.430056940459894E-4	0.0	0.0012674803016078644	4.370621729682291E-5
78-79	0.27832119174616826	7.430056940459894E-4	0.0	0.0012674803016078644	4.370621729682291E-5
80-81	0.3400125174606338	7.64858802694401E-4	0.0	0.0012893334102562757	6.555932594523436E-5
82-83	0.41689175368574527	7.867119113428124E-4	0.0	0.0013111865189046872	8.741243459364582E-5
84-85	0.5129580193041621	7.867119113428124E-4	0.0	0.0013330396275530987	8.741243459364582E-5
86-87	0.6236221614997176	7.867119113428124E-4	0.0	0.0014204520621467443	8.741243459364582E-5
88	0.7192295118365177	7.867119113428124E-4	0.0	0.0014860113880919789	8.741243459364582E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	2635	0.0	85.740654	1
AACGAGT	30850	0.0	83.0442	1
ACGAGTG	34315	0.0	81.415375	2
CGAGTGG	35685	0.0	80.4203	3
GTGGAAT	38360	0.0	79.3394	6
TGGAATC	38760	0.0	78.368256	7
GAGTGGA	37780	0.0	78.2858	4
GGAATCA	38835	0.0	78.19099	8
GAATCAC	38955	0.0	78.034584	9
AGTGGAA	38100	0.0	77.41853	5
ATCGAGT	865	0.0	70.19396	1
AACGTGT	1240	0.0	69.463326	1
ACGTGTG	1420	0.0	65.87937	2
CGTGTGG	1555	0.0	62.87799	3
TCGAGTG	990	0.0	62.204357	2
AGTACTT	21855	0.0	40.826305	20-21
ATGAGTG	2205	0.0	40.72016	2
GAGTACT	21540	0.0	40.51786	20-21
ATCACTG	39725	0.0	39.899582	10-11
TGAGTAC	39590	0.0	39.756664	18-19
>>END_MODULE