FastQCFastQC Report
Thu 26 May 2016
SRR2132759_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132759_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329315
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1004967.559983901483094No Hit
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT991517.4588039704659925No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG101920.7667106742946554No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81120.6102391081120728No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT78800.592786510345554No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33600.2527617607564799No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28290.21281637535121473No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27160.20431575661148788No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC26370.19837284616513015No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25530.19205380214621817No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24360.1832522765484479No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22580.1698619213655153No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21100.15872836761790846No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20050.1508295625942685No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA17760.13360264497128221No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14070.10584398731677593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT18050.086.620721
AACGTGT9150.071.0262451
ATCGAGT6600.070.6402361
ACGTGTG10150.068.096572
GTGGAAT347350.066.930476
TGGAATC350750.066.2483447
GAATCAC352300.066.127819
GGAATCA354250.065.7372748
CGTGTGG11000.064.9717943
CGAGTGG338500.064.909923
ACGAGTG329150.064.839592
AACGAGT304350.064.8028641
AGTGGAA347900.064.718985
GAGTGGA351700.064.166764
TCGAGTG7700.063.5062682
ATGAGTG11550.055.7715232
TGAGTGG12650.054.267063
AATGAGT11150.052.3730281
GTGTGGA15750.048.060314
AGCGAGT3650.043.867911
Produced by FastQC (version 0.11.5)