FastQCFastQC Report
Thu 26 May 2016
SRR2132758_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132758_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2379641
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1475856.201985929810421No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA611672.5704297412929096No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG165000.6933819008833686No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT121810.5118839354339583No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50240.21112428303260872No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT49900.2096954960853339No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41610.17485830845913314No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38240.16069650842290917No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35840.15061095350096926No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC33130.1392226810682788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT28100.086.280551
AACGTGT13800.068.5691761
GTGGAAT526200.067.2575156
TGGAATC527700.067.0264747
ATCGAGT10900.066.9448551
GGAATCA530300.066.708578
GAATCAC530300.066.708579
ACGAGTG490900.065.916462
AACGAGT453150.065.844921
CGAGTGG509850.065.371063
AGTGGAA524700.065.265245
GAGTGGA531850.064.440874
ACGTGTG15900.064.161092
TCGAGTG11900.063.2094732
CGTGTGG17650.061.790973
TGAGTGG22600.047.425093
AATGAGT19950.045.5435031
ATGAGTG21650.045.16632
AGTACTT241650.041.70811520-21
GAGTACT240550.041.0782620-21
Produced by FastQC (version 0.11.5)