##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132758_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2379641
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.81219730203001	31.0	25.0	33.0	16.0	34.0
2	28.084919531979825	31.0	25.0	33.0	16.0	34.0
3	29.14713059658999	31.0	28.0	34.0	16.0	34.0
4	34.67697186256246	35.0	35.0	37.0	32.0	37.0
5	33.77634861729143	35.0	35.0	37.0	28.0	37.0
6	35.20033988320087	37.0	35.0	37.0	32.0	37.0
7	35.60715755023552	37.0	35.0	37.0	33.0	37.0
8	35.875790087664484	37.0	35.0	37.0	35.0	37.0
9	37.83739816215975	39.0	38.0	39.0	35.0	39.0
10-11	37.83415460567372	39.0	38.0	39.0	35.0	39.0
12-13	37.806508208591126	39.0	38.0	39.0	35.0	39.0
14-15	39.209664398957656	41.0	39.0	41.0	36.0	41.0
16-17	39.1811163952882	40.0	39.0	41.0	36.0	41.0
18-19	39.03520636095949	40.0	39.0	41.0	35.5	41.0
20-21	38.96627306387812	40.0	39.0	41.0	35.0	41.0
22-23	38.831192184031124	40.0	39.0	41.0	35.0	41.0
24-25	38.682701088105304	40.0	38.5	41.0	35.0	41.0
26-27	38.497152511660374	40.0	38.0	41.0	34.5	41.0
28-29	38.324913715976486	40.0	38.0	41.0	34.0	41.0
30-31	38.207433180046905	40.0	38.0	41.0	34.0	41.0
32-33	38.043297077164155	40.0	38.0	41.0	34.0	41.0
34-35	37.938893513769514	40.0	38.0	41.0	33.5	41.0
36-37	37.77796566793059	40.0	37.5	41.0	33.0	41.0
38-39	37.34916548336493	40.0	36.0	41.0	33.0	41.0
40-41	37.1144136867704	40.0	35.0	41.0	33.0	41.0
42-43	36.96824521009682	40.0	35.0	41.0	33.0	41.0
44-45	36.821346371154306	40.0	35.0	41.0	32.5	41.0
46-47	36.69087543036954	39.0	35.0	41.0	32.0	41.0
48-49	36.52545573050725	39.0	35.0	41.0	31.0	41.0
50-51	36.25763550048096	39.0	35.0	41.0	31.0	41.0
52-53	35.87602982970961	39.0	35.0	41.0	30.0	41.0
54-55	35.13025242042812	39.0	35.0	41.0	27.0	41.0
56-57	34.52990619173228	38.5	35.0	40.0	22.5	41.0
58-59	34.20548897921997	38.0	35.0	40.0	21.0	41.0
60-61	33.95089889609399	37.5	34.0	40.0	20.0	41.0
62-63	33.66918518381554	37.0	34.0	40.0	19.0	41.0
64-65	33.3789968738982	36.5	34.0	40.0	18.0	41.0
66-67	33.05412602153014	36.0	34.0	39.0	16.5	41.0
68-69	32.741852657606756	35.0	34.0	39.0	14.0	41.0
70-71	32.420902774830324	35.0	33.0	39.0	11.0	40.0
72-73	32.11002668049508	35.0	33.0	37.5	7.5	40.0
74-75	31.77997500463305	35.0	33.0	37.0	4.5	39.5
76-77	31.456007860009137	35.0	33.0	36.5	2.0	39.0
78-79	31.136353550808714	35.0	33.0	36.0	2.0	39.0
80-81	30.841901362432402	35.0	33.0	36.0	2.0	37.5
82-83	30.56976850709834	35.0	32.5	35.0	2.0	37.0
84-85	30.309740628943608	35.0	32.0	35.0	2.0	37.0
86-87	30.12482260979702	35.0	32.0	35.0	2.0	36.0
88-89	29.92696818553723	35.0	32.0	35.0	2.0	36.0
90-91	29.744342108746658	35.0	32.0	35.0	2.0	36.0
92-93	29.59274382144197	35.0	32.0	35.0	2.0	35.5
94-95	29.418441269082187	35.0	32.0	35.0	2.0	35.0
96-97	29.229306227283864	35.0	31.5	35.0	2.0	35.0
98-99	29.071125644582523	35.0	31.0	35.0	2.0	35.0
100	28.977365913597893	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.14771343197607933
1101	2	2.2078801731565
1101	3	1.228382465098175
1101	4	0.6172041836278268
1101	5	2.2430032844984424
1101	6	0.9898672752537365
1101	7	0.5102465640939826
1101	8	0.34158852225471037
1101	9	0.26635219256987597
1101	10-11	0.4677767093042746
1101	12-13	0.40718575477652763
1101	14-15	0.46056348788637536
1101	16-17	0.41599671995799525
1101	18-19	0.36108322714049734
1101	20-21	0.35438783133822227
1101	22-23	0.3789431997982007
1101	24-25	0.34383786307033404
1101	26-27	0.2788051891631582
1101	28-29	0.3998955960634589
1101	30-31	0.49339754349126963
1101	32-33	0.4514246182873265
1101	34-35	0.41716891125415856
1101	36-37	0.4259670922809278
1101	38-39	0.4180656845452475
1101	40-41	0.46582873014754256
1101	42-43	0.5046789760902968
1101	44-45	0.5839720633054313
1101	46-47	0.5429603872978532
1101	48-49	0.5205925744919782
1101	50-51	0.5659364144585481
1101	52-53	0.6993796594460662
1101	54-55	0.7384580451281479
1101	56-57	0.5584675852573469
1101	58-59	0.5643651660000657
1101	60-61	0.577457615096634
1101	62-63	0.5758535401676639
1101	64-65	0.5989519347392402
1101	66-67	0.6792266283743871
1101	68-69	0.6683383698960057
1101	70-71	0.6503204609354682
1101	72-73	0.6878268004385149
1101	74-75	0.6466084453703616
1101	76-77	0.5484212190339868
1101	78-79	0.6087238099333838
1101	80-81	0.5895916012722822
1101	82-83	0.60246269879595
1101	84-85	0.5465361228023795
1101	86-87	0.5115587961157644
1101	88-89	0.5551795277533031
1101	90-91	0.5365308563404803
1101	92-93	0.5184435916948331
1101	94-95	0.45741254514696905
1101	96-97	0.41317614017442494
1101	98-99	0.5291502509538013
1101	100	0.56073673950252
1102	1	1.569341222538327
1102	2	3.139322379594976
1102	3	3.2768421803323875
1102	4	1.440616190810303
1102	5	2.701651259246546
1102	6	1.2557461501257947
1102	7	0.739311043892549
1102	8	0.49764552913041626
1102	9	0.41629819761467246
1102	10-11	0.4903827683782609
1102	12-13	0.48094443037414436
1102	14-15	0.64222958708595
1102	16-17	0.5307546958989704
1102	18-19	0.42334029263251693
1102	20-21	0.3642426018932383
1102	22-23	0.3081285066399033
1102	24-25	0.3196758776780726
1102	26-27	0.28291155751329455
1102	28-29	0.3462431618929287
1102	30-31	0.3504693026682375
1102	32-33	0.2543677185852218
1102	34-35	0.2981644118062192
1102	36-37	0.31160153761278764
1102	38-39	0.3111220333697773
1102	40-41	0.4502830282434118
1102	42-43	0.40735711796866525
1102	44-45	0.42768997870253855
1102	46-47	0.5015697189848751
1102	48-49	0.44886486495508393
1102	50-51	0.6055065140246683
1102	52-53	0.6810541587386325
1102	54-55	0.727849767797629
1102	56-57	0.6497409155940232
1102	58-59	0.5864756459192364
1102	60-61	0.43161764924472834
1102	62-63	0.4826360754526533
1102	64-65	0.5357041717577431
1102	66-67	0.5532022580982883
1102	68-69	0.5403318804949961
1102	70-71	0.48874157787398076
1102	72-73	0.502894078863676
1102	74-75	0.497156373694132
1102	76-77	0.48056551788559787
1102	78-79	0.5112627182952139
1102	80-81	0.5155853994230526
1102	82-83	0.5955650111387918
1102	84-85	0.5379464352200074
1102	86-87	0.5395738304389646
1102	88-89	0.5982959675252921
1102	90-91	0.6352607175124838
1102	92-93	0.6487736021199026
1102	94-95	0.6445146104902157
1102	96-97	0.6138521238345334
1102	98-99	0.6673306617789123
1102	100	0.6424546348868674
1103	1	1.6032467206190546
1103	2	3.0652365310253096
1103	3	3.279232227617051
1103	4	1.4665808470425077
1103	5	2.647780143814991
1103	6	1.2760599110496216
1103	7	0.7350807460354005
1103	8	0.530819711116095
1103	9	0.45577689616361994
1103	10-11	0.5099234940442798
1103	12-13	0.5059091836775664
1103	14-15	0.627891607083626
1103	16-17	0.4589844817654196
1103	18-19	0.38847935629797803
1103	20-21	0.36633976298310955
1103	22-23	0.319816720212728
1103	24-25	0.39658266116344265
1103	26-27	0.43335155267109116
1103	28-29	0.43027095203077437
1103	30-31	0.45947797204911467
1103	32-33	0.45672964172628383
1103	34-35	0.4385349726443124
1103	36-37	0.4457919819931817
1103	38-39	0.40959721374992597
1103	40-41	0.43189311400563923
1103	42-43	0.49540376996482394
1103	44-45	0.5419150897506171
1103	46-47	0.505361492268932
1103	48-49	0.5743490691712054
1103	50-51	0.559136201103172
1103	52-53	0.6199478296576331
1103	54-55	0.5876089087438956
1103	56-57	0.6598857410482424
1103	58-59	0.6419271034327778
1103	60-61	0.6507123333145302
1103	62-63	0.6143591563995727
1103	64-65	0.6716449906323234
1103	66-67	0.703968338058452
1103	68-69	0.6336643062314167
1103	70-71	0.6126070028369881
1103	72-73	0.6671740433525244
1103	74-75	0.7031815675241404
1103	76-77	0.6518751928560853
1103	78-79	0.6499348169104806
1103	80-81	0.6631420702666802
1103	82-83	0.6875772873022292
1103	84-85	0.6262604748606613
1103	86-87	0.7060053928979784
1103	88-89	0.6939144266579973
1103	90-91	0.7223549923431811
1103	92-93	0.7524867464584872
1103	94-95	0.7775340465708389
1103	96-97	0.8186660102945353
1103	98-99	0.8623960232732486
1103	100	0.9358802123816936
1104	1	6.081759621571564
1104	2	5.092535173867841
1104	3	4.152189739722353
1104	4	1.865793741175132
1104	5	2.8838678930250197
1104	6	1.3341360664521034
1104	7	0.7048641009650396
1104	8	0.5559856389616868
1104	9	0.4454394026993427
1104	10-11	0.47035241945745554
1104	12-13	0.324367525535763
1104	14-15	0.49926135861241505
1104	16-17	0.3441667773393178
1104	18-19	0.3151263816906891
1104	20-21	0.3092713140526868
1104	22-23	0.27017476426919984
1104	24-25	0.2586648180663005
1104	26-27	0.32941675078880195
1104	28-29	0.2605686173561992
1104	30-31	0.40912865744773086
1104	32-33	0.4665445499988081
1104	34-35	0.4322166657948685
1104	36-37	0.4374806314282722
1104	38-39	0.4276086256938143
1104	40-41	0.4795952213506567
1104	42-43	0.5021847379340869
1104	44-45	0.558360928483026
1104	46-47	0.48224534959689436
1104	48-49	0.572545436444841
1104	50-51	0.5483966980455932
1104	52-53	0.3897560999070038
1104	54-55	0.5369689760334424
1104	56-57	0.44871731257749303
1104	58-59	0.42479054656629245
1104	60-61	0.3124007554200574
1104	62-63	0.4117563238732558
1104	64-65	0.37725211968731287
1104	66-67	0.4336635800507693
1104	68-69	0.373177275382794
1104	70-71	0.44440458859705956
1104	72-73	0.38170331203333774
1104	74-75	0.4396745998731362
1104	76-77	0.4349208633646171
1104	78-79	0.43164997297222385
1104	80-81	0.5163087255668728
1104	82-83	0.4913914226542495
1104	84-85	0.4689463281421631
1104	86-87	0.5168432841889867
1104	88-89	0.46027565332829923
1104	90-91	0.5442321889150499
1104	92-93	0.5867985971484302
1104	94-95	0.5599936614745644
1104	96-97	0.5221094166006601
1104	98-99	0.5073917267445722
1104	100	0.5316382427983903
1105	1	1.634761497150624
1105	2	3.102598220440953
1105	3	3.283822487784626
1105	4	1.4346692524399103
1105	5	2.6363156818754945
1105	6	1.209588035810114
1105	7	0.742542902402235
1105	8	0.5055156595252654
1105	9	0.40377153837376056
1105	10-11	0.4241201640171397
1105	12-13	0.38074273173646844
1105	14-15	0.42302318266953876
1105	16-17	0.38801763490829444
1105	18-19	0.40807709689643445
1105	20-21	0.38984591916420896
1105	22-23	0.28769674018043645
1105	24-25	0.38916525871439234
1105	26-27	0.38068504898327404
1105	28-29	0.4391592665777182
1105	30-31	0.4767564340956483
1105	32-33	0.4659616290668893
1105	34-35	0.545665968666988
1105	36-37	0.5425021214967458
1105	38-39	0.5116140490549483
1105	40-41	0.4716062488516144
1105	42-43	0.5349310828146585
1105	44-45	0.6626595749024773
1105	46-47	0.6166274705830475
1105	48-49	0.5563246152575729
1105	50-51	0.4379259954943535
1105	52-53	0.5265424872705751
1105	54-55	0.575558450193391
1105	56-57	0.5415805308903714
1105	58-59	0.405124987169188
1105	60-61	0.3732051302647008
1105	62-63	0.41146689716772045
1105	64-65	0.47496065922135955
1105	66-67	0.4810947598577897
1105	68-69	0.5092364804084468
1105	70-71	0.5403576765233069
1105	72-73	0.45938943500804896
1105	74-75	0.4813693098363032
1105	76-77	0.4444932765343452
1105	78-79	0.4665168520538643
1105	80-81	0.46732456630083163
1105	82-83	0.3803490074507607
1105	84-85	0.3668037389391685
1105	86-87	0.37684834599270545
1105	88-89	0.35559737162988725
1105	90-91	0.36408141947569206
1105	92-93	0.4206406417555755
1105	94-95	0.38233718620735146
1105	96-97	0.44746489532526823
1105	98-99	0.459337764301349
1105	100	0.5033554145155605
1106	1	-0.6518521842186793
1106	2	1.8437889758279624
1106	3	2.7054279958899734
1106	4	1.2229450310253114
1106	5	2.49110547328862
1106	6	1.1392260743376497
1106	7	0.6442580403589844
1106	8	0.4601388438732599
1106	9	0.3166625636771485
1106	10-11	0.41629767123170325
1106	12-13	0.3209483523916816
1106	14-15	0.38823150618479474
1106	16-17	0.34095488142853725
1106	18-19	0.23563240850155154
1106	20-21	0.2739228855295366
1106	22-23	0.2795247699017338
1106	24-25	0.196022483944418
1106	26-27	0.15601177162917423
1106	28-29	0.2057694735012916
1106	30-31	0.2781934315831407
1106	32-33	0.25058600544546294
1106	34-35	0.35129383150400173
1106	36-37	0.31341213982074834
1106	38-39	0.36198149941241553
1106	40-41	0.30330548382671196
1106	42-43	0.3253362530856023
1106	44-45	0.4843890911505966
1106	46-47	0.3615410576063596
1106	48-49	0.4120768096565115
1106	50-51	0.4411825491214074
1106	52-53	0.5004547593640325
1106	54-55	0.44808759718924307
1106	56-57	0.35550834558414124
1106	58-59	0.3151656732982957
1106	60-61	0.3414515608723505
1106	62-63	0.33694816796472793
1106	64-65	0.2976941609174659
1106	66-67	0.297418001589584
1106	68-69	0.32044008939654134
1106	70-71	0.25232483192251465
1106	72-73	0.32532120095977746
1106	74-75	0.30088266566112765
1106	76-77	0.34079807413402463
1106	78-79	0.4093311715753529
1106	80-81	0.43020260863547577
1106	82-83	0.4974362576210183
1106	84-85	0.4283195398127724
1106	86-87	0.43567126661547917
1106	88-89	0.4647758223043077
1106	90-91	0.4352417642220239
1106	92-93	0.4060740280372457
1106	94-95	0.3724992376827423
1106	96-97	0.4493938595430933
1106	98-99	0.44696725901899015
1106	100	0.4658502513598428
1107	1	-1.0842124003380391
1107	2	1.7037374436396817
1107	3	-0.7175152687498887
1107	4	-0.13293613233237522
1107	5	1.8824432959489243
1107	6	0.7929445938588131
1107	7	0.3641540091496154
1107	8	0.29712793316510044
1107	9	0.22844164882772588
1107	10-11	0.3056621881456323
1107	12-13	0.27222771389529754
1107	14-15	0.3217536941918837
1107	16-17	0.26107531656774796
1107	18-19	0.23081113560171218
1107	20-21	0.19104662240277293
1107	22-23	0.15543306903335719
1107	24-25	0.05065063199229769
1107	26-27	0.03521664833382232
1107	28-29	0.0027386138096758827
1107	30-31	0.01713149466253583
1107	32-33	0.06783059641879419
1107	34-35	0.0794739460092373
1107	36-37	0.13784883886019372
1107	38-39	0.19454829547353825
1107	40-41	0.1558207799273177
1107	42-43	0.1898789492277828
1107	44-45	0.15835115585426962
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2216	54-55	-0.08305652865681878
2216	56-57	-5.246361537558641E-4
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2216	72-73	0.13019395310885784
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2216	80-81	0.055025882874613075
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2216	84-85	0.09797299999622311
2216	86-87	0.026359732552791115
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2216	90-91	0.07693516734413564
2216	92-93	0.04056732580446365
2216	94-95	-0.012394355643838395
2216	96-97	-0.005977472259409922
2216	98-99	-0.04641677193696481
2216	100	-0.11750367567305631
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1252.0
3	285.0
4	379.0
5	911.0
6	2510.0
7	4319.0
8	5191.0
9	6945.0
10	5382.0
11	5334.0
12	4572.0
13	6313.0
14	6402.0
15	6673.0
16	7734.0
17	8941.0
18	12452.0
19	20835.0
20	40388.0
21	47146.0
22	22644.0
23	15895.0
24	16386.0
25	17467.0
26	18689.0
27	20826.0
28	24554.0
29	29125.0
30	34346.0
31	42520.0
32	55462.0
33	77638.0
34	115188.0
35	224967.0
36	325521.0
37	522098.0
38	503907.0
39	118078.0
40	366.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.696450265124467	39.806078947800295	24.68693599772095	20.81053478935429
2	15.929504784390394	39.320590928632754	24.66504644445556	20.08485784252129
3	19.349333490225085	25.16276118273967	19.045201633915514	36.44270369311974
4	31.6483452756109	26.860312122710944	21.169789896879404	20.321552704798748
5	18.24510281431899	37.94894983612661	21.579303247084255	22.226644102470143
6	35.89061868261278	23.831086357343573	22.94512861937692	17.33316634066672
7	19.90109903040572	23.05997095251543	37.67961364155942	19.359316375519427
8	34.82088264574362	23.573807981960304	22.71325800824578	18.892051364050293
9	34.297232229567406	24.994190300133507	22.849328953400956	17.859248516898138
10-11	17.841199575902415	40.986665635698834	22.734311604145333	18.437823184253425
12-13	17.71998381268435	24.893565878214403	30.59257677943858	26.793873529662665
14-15	17.962541408557005	32.62969078108841	22.71111482782487	26.696652982529717
16-17	26.24742555704831	24.441838075575266	30.741906027001555	18.568830340374873
18-19	18.218945630874572	24.627244193556926	30.87152641932123	26.282283756247267
20-21	26.376898868358715	32.81356305425903	22.717271218641805	18.09226685874046
22-23	25.90298284489131	24.865410370723986	30.925652230735643	18.30595455364906
24-25	18.048436717975527	32.806776316259466	22.601749591640083	26.54303737412492
26-27	18.465549088422847	28.152100349653804	34.96596858473731	18.41638197718604
28-29	25.76327689764969	24.609867622889332	31.33737399885109	18.28948148060989
30-31	23.957395254158083	25.742832637360003	31.47447451107121	18.8252975974107
32-33	20.359562639910813	26.99638306786612	32.271968754950855	20.37208553727222
34-35	20.258496974963872	26.74344575505297	32.74357350541531	20.254483764567848
36-37	20.489267078521507	26.56648628931843	32.95530712405779	19.98893950810227
38-39	21.272389406637387	26.337649250454163	32.401589147270535	19.98837219563791
40-41	19.9077297794079	27.714768740326797	32.2901437653831	20.087357714882202
42-43	20.047276455904726	26.72554861700608	32.89117590203478	20.33599902505442
44-45	20.105091482286614	27.203515152075457	32.07588875801014	20.615504607627788
46-47	20.630927102029258	26.450376338279597	32.668961410565714	20.249735149125435
48-49	20.01995258948724	26.85924053250049	32.55276741323586	20.56803946477641
50-51	20.61775284591247	27.370872329061402	31.806121175420998	20.20525364960513
52-53	20.847285046956763	27.019384477289805	31.756726659035245	20.376603816718188
54-55	20.81816122684052	27.98327562855069	30.076553564172077	21.122009580436714
56-57	21.163444401907682	28.676447413706523	29.080289842039196	21.0798183423466
58-59	22.211081419424193	28.927724812272103	27.888912655312293	20.972281112991414
60-61	21.653455290104684	29.380103973666614	27.707393678290128	21.25904705793857
62-63	20.86016774382517	29.48035638156268	27.506297694966953	22.153178179645202
64-65	21.303717661613664	29.36573205790285	28.131092042875377	21.19945823760811
66-67	21.417684432231585	28.744272770556567	28.698635634534792	21.13940716267706
68-69	21.748679737826	28.707565552955256	28.492743233117935	21.051011476100808
70-71	20.367715130139377	29.72870697722892	28.818821830687906	21.084756061943796
72-73	20.404978925295108	28.81508890037527	29.08000403424062	21.699928140089007
74-75	20.6443808772193	29.35055531672321	28.238556113484726	21.766507692572763
76-77	21.272131877783394	28.757505113720526	28.516879217440543	21.453483791055532
78-79	20.98921514821187	29.168624250620052	27.98482875938064	21.857331841787435
80-81	21.471264445100946	29.669803451233456	27.560742019815827	21.298190083849768
82-83	21.190104031512654	29.67286101473116	27.642869194624087	21.494165759132095
84-85	21.11249999737351	30.04247134345224	26.928403438470554	21.916625220703693
86-87	21.12338420937621	30.11625708090299	27.186843388075506	21.57351532164529
88-89	22.094299098057228	29.566539658713225	26.892333759588105	21.44682748364144
90-91	21.45952749155334	30.17172765628414	26.84813669294515	21.52060815921736
92-93	20.951143470800847	30.279945588431197	26.785910143588886	21.98300079717907
94-95	21.23094249550352	30.513460019162565	26.518254862079978	21.737342623253937
96-97	21.22305438542819	29.83909534679662	26.904557389284285	22.03329287849091
98-99	21.89470309063598	29.955957318458147	26.111714731343007	22.037624859562868
100	21.08855915661228	30.815530577931717	26.123730428245267	21.97217983721074
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	65528.0
1	35587.5
2	4077.0
3	2157.0
4	1759.0
5	1658.5
6	1661.5
7	1845.5
8	1774.0
9	1566.5
10	1616.5
11	2503.5
12	18282.0
13	20651.5
14	7123.5
15	6372.5
16	6853.0
17	7576.5
18	8449.0
19	9453.5
20	10618.5
21	11773.5
22	13162.0
23	14899.0
24	16617.5
25	18454.5
26	20629.0
27	22908.5
28	25058.0
29	27392.0
30	29920.0
31	32483.0
32	35144.5
33	37709.0
34	40980.0
35	44308.0
36	47066.5
37	49593.5
38	52140.5
39	54278.5
40	55643.0
41	57301.0
42	59439.5
43	61732.5
44	64525.0
45	67585.0
46	70710.0
47	74586.0
48	77203.5
49	82706.0
50	101370.0
51	103675.0
52	85072.0
53	76592.0
54	73916.0
55	72841.5
56	68330.0
57	61083.0
58	55640.5
59	48151.0
60	39877.0
61	32819.5
62	26704.5
63	20842.5
64	15868.0
65	12079.0
66	9103.5
67	6832.5
68	5271.5
69	3965.0
70	2787.5
71	2011.5
72	1437.5
73	1011.0
74	680.5
75	494.5
76	349.0
77	226.0
78	133.5
79	78.5
80	43.0
81	24.0
82	12.0
83	7.0
84	4.5
85	2.5
86	2.5
87	1.5
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13489429708094625
2	0.005547055207066948
3	0.003403874786154718
4	0.0
5	0.002521388730484976
6	0.012396827924884469
7	0.003740059950219382
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	6.303471826212441E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	8.824860556697419E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	2.101157275404147E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	2.101157275404147E-5
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	4.6225460058891237E-4
74-75	3.361851640646635E-4
76-77	5.673124643591198E-4
78-79	2.941620185565806E-4
80-81	0.0011766480742263223
82-83	0.0010715902104561149
84-85	0.0015338448110450275
86-87	4.202314550808294E-5
88-89	0.0
90-91	4.202314550808294E-5
92-93	0.0
94-95	4.202314550808294E-5
96-97	0.001386763801766737
98-99	7.354050463914515E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2379641.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.70657526055503
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.74240779493415	61.65893495226064
2	7.050501794795047	9.688316643775284
3	1.4586314804424652	3.0065272076530505
4	0.5619257589872539	1.5443197780280908
5	0.2959176811853278	1.0165745216644329
6	0.17632023270876546	0.7268615603138022
7	0.12239655284788174	0.588661357891284
8	0.07507314790907621	0.4126415109489379
9	0.05881755790735096	0.36370376721031156
>10	0.4221225197807631	5.395640719000632
>50	0.019670867760133975	0.9273552804987675
>100	0.012485050526571274	1.7668310643294292
>500	0.002139911598923773	1.0329626497592
>1k	0.0012839469593537531	1.6856740630144438
>5k	6.114033139779777E-5	0.21104531729925652
>10k+	2.4456132559119107E-4	9.973949606352384
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	147585	6.201985929810421	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	61167	2.5704297412929096	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	16500	0.6933819008833686	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12181	0.5118839354339583	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5024	0.21112428303260872	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4990	0.2096954960853339	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4161	0.17485830845913314	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3824	0.16069650842290917	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3584	0.15061095350096926	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	3313	0.1392226810682788	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.1439347363741E-4	0.0	0.0	0.0	0.0
2	7.1439347363741E-4	0.0	0.0	0.0	4.202314550808294E-5
3	7.1439347363741E-4	0.0	0.0	0.0	4.202314550808294E-5
4	7.1439347363741E-4	0.0	0.0	0.0	4.202314550808294E-5
5	7.56416619145493E-4	0.0	0.0	0.0	4.202314550808294E-5
6	7.56416619145493E-4	0.0	0.0	0.0	4.202314550808294E-5
7	7.56416619145493E-4	0.0	0.0	0.0	4.202314550808294E-5
8	7.56416619145493E-4	0.0	0.0	0.0	4.202314550808294E-5
9	7.56416619145493E-4	0.0	0.0	0.0	4.202314550808294E-5
10-11	7.56416619145493E-4	0.0	0.0	0.0	8.404629101616588E-5
12-13	7.774281918995344E-4	0.0	0.0	0.0	1.0505786377020736E-4
14-15	7.984397646535759E-4	0.0	0.0	0.0	1.6809258203233176E-4
16-17	7.984397646535759E-4	0.0	0.0	0.0	1.6809258203233176E-4
18-19	7.984397646535759E-4	0.0	0.0	0.0	1.8910415478637322E-4
20-21	8.194513374076174E-4	0.0	0.0	0.0	2.5213887304849765E-4
22-23	9.034976284237832E-4	0.0	0.0	0.0	2.5213887304849765E-4
24-25	9.455207739318661E-4	0.0	0.0	0.0	2.5213887304849765E-4
26-27	0.0010085554921939906	0.0	0.0	2.101157275404147E-5	2.5213887304849765E-4
28-29	0.001071590210456115	0.0	0.0	4.202314550808294E-5	3.57196736818705E-4
30-31	0.0011766480742263223	0.0	0.0	4.202314550808294E-5	3.782083095727465E-4
32-33	0.0012817059379965298	0.0	0.0	4.202314550808294E-5	3.9921988232678794E-4
34-35	0.0015968795293071518	0.0	0.0	4.202314550808294E-5	4.412430278348709E-4
36-37	0.0021852035664203127	0.0	0.0	4.202314550808294E-5	4.6225460058891237E-4
38-39	0.0033198284951385525	0.0	0.0	4.202314550808294E-5	4.6225460058891237E-4
40-41	0.005126823751986118	0.0	0.0	4.202314550808294E-5	4.6225460058891237E-4
42-43	0.007480119900438764	0.0	0.0	4.202314550808294E-5	5.042777460969953E-4
44-45	0.009812404476137366	0.0	0.0	4.202314550808294E-5	6.303471826212442E-4
46-47	0.012501885788654675	0.0	0.0	4.202314550808294E-5	7.1439347363741E-4
48-49	0.015506540692482605	0.0	0.0	4.202314550808294E-5	7.354050463914515E-4
50-51	0.019645820525028775	0.0	0.0	4.202314550808294E-5	7.56416619145493E-4
52-53	0.025339956741374015	0.0	0.0	4.202314550808294E-5	7.56416619145493E-4
54-55	0.031769498004110705	0.0	0.0	4.202314550808294E-5	7.774281918995344E-4
56-57	0.03939669891382776	0.0	0.0	4.202314550808294E-5	8.404629101616589E-4
58-59	0.047759304869936264	0.0	0.0	4.202314550808294E-5	8.404629101616589E-4
60-61	0.05868532270203783	0.0	0.0	4.202314550808294E-5	8.404629101616589E-4
62-63	0.07217475241013245	0.0	0.0	4.202314550808294E-5	8.404629101616589E-4
64-65	0.08936221892293839	0.0	0.0	8.404629101616588E-5	8.824860556697417E-4
66-67	0.10778936822823275	0.0	0.0	8.404629101616588E-5	8.824860556697417E-4
68-69	0.1331503365423608	0.0	0.0	8.404629101616588E-5	9.245092011778247E-4
70-71	0.16716807283115392	0.0	0.0	8.404629101616588E-5	0.0010085554921939906
72-73	0.20585037827134428	0.0	0.0	8.404629101616588E-5	0.0010505786377020736
74-75	0.2500377157730935	0.0	0.0	8.404629101616588E-5	0.0010926017832101566
76-77	0.3010538144199062	0.0	0.0	8.404629101616588E-5	0.0010926017832101566
78-79	0.36293289618055835	0.0	0.0	1.2606943652424882E-4	0.0011346249287182393
80-81	0.4365364355379656	0.0	0.0	1.2606943652424882E-4	0.0011346249287182393
82-83	0.5324542651601649	0.0	0.0	1.2606943652424882E-4	0.0011346249287182393
84-85	0.6452864108493677	0.0	0.0	1.2606943652424882E-4	0.0011346249287182393
86-87	0.7768818910079294	0.0	0.0	1.2606943652424882E-4	0.0011346249287182393
88	0.884629236090654	0.0	0.0	1.2606943652424882E-4	0.0011766480742263223
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	2810	0.0	86.28055	1
AACGTGT	1380	0.0	68.569176	1
GTGGAAT	52620	0.0	67.257515	6
TGGAATC	52770	0.0	67.026474	7
ATCGAGT	1090	0.0	66.944855	1
GGAATCA	53030	0.0	66.70857	8
GAATCAC	53030	0.0	66.70857	9
ACGAGTG	49090	0.0	65.91646	2
AACGAGT	45315	0.0	65.84492	1
CGAGTGG	50985	0.0	65.37106	3
AGTGGAA	52470	0.0	65.26524	5
GAGTGGA	53185	0.0	64.44087	4
ACGTGTG	1590	0.0	64.16109	2
TCGAGTG	1190	0.0	63.209473	2
CGTGTGG	1765	0.0	61.79097	3
TGAGTGG	2260	0.0	47.42509	3
AATGAGT	1995	0.0	45.543503	1
ATGAGTG	2165	0.0	45.1663	2
AGTACTT	24165	0.0	41.708115	20-21
GAGTACT	24055	0.0	41.07826	20-21
>>END_MODULE