FastQCFastQC Report
Thu 26 May 2016
SRR2132757_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132757_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2224906
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1321765.940745361826522No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA127060.5710803063140645No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT105140.4725592901452916No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG48460.21780695454100082No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42120.18931136866006923No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36830.16553508327992283No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35920.16144502284590898No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29640.13321911127930797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT29750.086.701091
AACGAGT325050.081.973591
ACGAGTG364200.080.390072
CGAGTGG378750.079.5323943
GTGGAAT402400.079.258626
TGGAATC405650.078.564917
GAATCAC406800.078.365919
GGAATCA408500.078.005278
GAGTGGA397250.077.9704064
AGTGGAA402500.076.906685
ATCGAGT8950.071.5231861
AACGTGT13700.061.8419081
ACGTGTG14800.060.986432
CGTGTGG15700.060.7813763
TCGAGTG11300.059.907022
TGAGTGG22100.043.8176543
ATGAGTG20050.043.6106222
AGTACTT222300.041.24811620-21
GAGTACT218300.041.04586420-21
GTGTGGA25550.040.476784