##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132757_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2224906
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.76382417953837	31.0	23.0	33.0	16.0	34.0
2	28.064024277879604	31.0	25.0	33.0	16.0	34.0
3	29.15602142292753	31.0	28.0	34.0	16.0	34.0
4	34.69846501380283	35.0	35.0	37.0	32.0	37.0
5	33.793698700079915	35.0	35.0	37.0	28.0	37.0
6	35.23188620103501	37.0	35.0	37.0	32.0	37.0
7	35.64642011842298	37.0	35.0	37.0	33.0	37.0
8	35.91594386459473	37.0	35.0	37.0	35.0	37.0
9	37.885177620987136	39.0	38.0	39.0	35.0	39.0
10-11	37.88144937359151	39.0	38.0	39.0	35.0	39.0
12-13	37.863579629880995	39.0	38.0	39.0	35.0	39.0
14-15	39.28736157842174	41.0	39.0	41.0	36.0	41.0
16-17	39.312237910275755	41.0	39.0	41.0	36.0	41.0
18-19	39.297318178835425	41.0	39.0	41.0	36.0	41.0
20-21	39.293454644825445	40.5	39.0	41.0	36.0	41.0
22-23	39.20775417028854	40.0	39.0	41.0	36.0	41.0
24-25	39.1453202966777	40.0	39.0	41.0	36.0	41.0
26-27	39.003175415051246	40.0	39.0	41.0	35.5	41.0
28-29	38.845304026327405	40.0	39.0	41.0	35.0	41.0
30-31	38.73174934132049	40.0	39.0	41.0	35.0	41.0
32-33	38.59202096627902	40.0	38.0	41.0	35.0	41.0
34-35	38.50975299630636	40.0	38.0	41.0	35.0	41.0
36-37	38.373709945498824	40.0	38.0	41.0	34.0	41.0
38-39	37.95501877382685	40.0	37.0	41.0	34.0	41.0
40-41	37.72441262687053	40.0	36.0	41.0	33.0	41.0
42-43	37.579626285335195	40.0	36.0	41.0	33.0	41.0
44-45	37.440167809336664	40.0	36.0	41.0	33.0	41.0
46-47	37.310843469342075	40.0	35.0	41.0	33.0	41.0
48-49	37.15686078423089	40.0	35.0	41.0	33.0	41.0
50-51	36.896632262216926	40.0	35.0	41.0	32.5	41.0
52-53	36.52287647208466	39.5	35.0	41.0	31.5	41.0
54-55	35.76156857862759	39.0	35.0	41.0	29.0	41.0
56-57	35.08585014378136	39.0	35.0	41.0	25.0	41.0
58-59	34.73900537820474	39.0	35.0	41.0	23.0	41.0
60-61	34.483047598415396	38.5	35.0	40.5	22.5	41.0
62-63	34.19649189673631	38.0	35.0	40.0	22.0	41.0
64-65	33.91484494176383	37.0	34.0	40.0	21.0	41.0
66-67	33.60729284742816	37.0	34.0	40.0	20.0	41.0
68-69	33.281874829768086	36.0	34.0	39.5	19.0	41.0
70-71	32.94150606812153	36.0	34.0	39.0	18.0	41.0
72-73	32.59896036057253	35.0	34.0	39.0	14.0	40.0
74-75	32.25136455203051	35.0	33.5	37.5	9.5	40.0
76-77	31.91200819270567	35.0	33.0	37.0	7.0	39.0
78-79	31.574661131751185	35.0	33.0	36.5	2.0	39.0
80-81	31.266809249469414	35.0	33.0	36.0	2.0	38.5
82-83	30.98213115520386	35.0	33.0	36.0	2.0	37.0
84-85	30.7227397472073	35.0	33.0	35.0	2.0	37.0
86-87	30.513320787484957	35.0	33.0	35.0	2.0	36.5
88-89	30.317849832756977	35.0	33.0	35.0	2.0	36.0
90-91	30.136123728373242	35.0	32.5	35.0	2.0	36.0
92-93	29.970805732916357	35.0	32.0	35.0	2.0	36.0
94-95	29.80101900934242	35.0	32.0	35.0	2.0	35.5
96-97	29.645699189089335	35.0	32.0	35.0	2.0	35.0
98-99	29.493393878213283	35.0	32.0	35.0	2.0	35.0
100	29.39210780140824	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.076392793629104
1101	2	2.209809930231632
1101	3	1.2516694104541308
1101	4	0.62888408334031
1101	5	2.259147746391818
1101	6	1.0383069625658194
1101	7	0.5799396981829119
1101	8	0.41784149024420003
1101	9	0.3488154205143843
1101	10-11	0.4450123023055781
1101	12-13	0.3557513055421566
1101	14-15	0.48948396297877395
1101	16-17	0.416389406417494
1101	18-19	0.4177055932439231
1101	20-21	0.43301175241660417
1101	22-23	0.3364323685229138
1101	24-25	0.43396168430363247
1101	26-27	0.34505186631898255
1101	28-29	0.42676114895604655
1101	30-31	0.3869595999497477
1101	32-33	0.42648246750125196
1101	34-35	0.4293764758986498
1101	36-37	0.5304780446602848
1101	38-39	0.4831476860882873
1101	40-41	0.38808307635837735
1101	42-43	0.4781406927993288
1101	44-45	0.5838915174340258
1101	46-47	0.519033730418947
1101	48-49	0.4436546789071585
1101	50-51	0.37710221152308065
1101	52-53	0.5721984228890946
1101	54-55	0.6510408491409834
1101	56-57	0.6825348058017227
1101	58-59	0.6798328649349514
1101	60-61	0.6741051116681405
1101	62-63	0.6468273862073204
1101	64-65	0.6496848875995553
1101	66-67	0.6542910737496683
1101	68-69	0.7461096269219851
1101	70-71	0.7205668795581275
1101	72-73	0.7483473265273375
1101	74-75	0.6846568073883645
1101	76-77	0.5914505719501193
1101	78-79	0.6343418582408908
1101	80-81	0.6349881844869074
1101	82-83	0.5965485092677483
1101	84-85	0.6482068343085317
1101	86-87	0.5812652935651101
1101	88-89	0.5558717913234617
1101	90-91	0.5457112837006726
1101	92-93	0.5579873536179605
1101	94-95	0.5767737243810451
1101	96-97	0.5746561476970165
1101	98-99	0.5803173738793959
1101	100	0.688898850970947
1102	1	1.504232843176709
1102	2	3.1263514055373527
1102	3	3.255304513567026
1102	4	1.4522470280629491
1102	5	2.65871339146198
1102	6	1.2301947989936508
1102	7	0.702302425735482
1102	8	0.49091260113085156
1102	9	0.3857614695386218
1102	10-11	0.48854543678694284
1102	12-13	0.4617559951438679
1102	14-15	0.586444151349589
1102	16-17	0.47838617467247957
1102	18-19	0.43848197365845465
1102	20-21	0.4064370716074279
1102	22-23	0.37543354739334234
1102	24-25	0.39012597579126407
1102	26-27	0.4408605800813632
1102	28-29	0.47857728450743764
1102	30-31	0.5030966006203315
1102	32-33	0.46075625988000724
1102	34-35	0.4626812475842854
1102	36-37	0.49710413643530416
1102	38-39	0.5688235342106154
1102	40-41	0.6184599266943351
1102	42-43	0.5903879312752096
1102	44-45	0.567414320624188
1102	46-47	0.7386691287115923
1102	48-49	0.6535855623323812
1102	50-51	0.7210570123458879
1102	52-53	0.7766858205550022
1102	54-55	0.8591549204379874
1102	56-57	0.898676590572947
1102	58-59	0.8415887422617345
1102	60-61	0.8336352730564798
1102	62-63	0.8171161913452849
1102	64-65	0.8534802913580535
1102	66-67	0.839019544243115
1102	68-69	0.8558651401543145
1102	70-71	0.876541906996458
1102	72-73	0.833078555088079
1102	74-75	0.9034620496091463
1102	76-77	0.8623801803792475
1102	78-79	0.8439260237698853
1102	80-81	0.7795160126094771
1102	82-83	0.7926939734338383
1102	84-85	0.8116944895172864
1102	86-87	0.8274313027445181
1102	88-89	0.861389560042582
1102	90-91	0.8721920275477224
1102	92-93	0.839461393438981
1102	94-95	0.8854512917479447
1102	96-97	0.854070805959541
1102	98-99	0.8133355357894771
1102	100	0.8287731614818519
1103	1	1.3602577822689632
1103	2	3.0666049926399417
1103	3	3.2646523214624494
1103	4	1.456075453808431
1103	5	2.657174815890329
1103	6	1.2609321388823034
1103	7	0.6358887806882265
1103	8	0.4616610457013621
1103	9	0.35604499562253267
1103	10-11	0.468220002407044
1103	12-13	0.46381160259086585
1103	14-15	0.640976835504425
1103	16-17	0.44599376206798524
1103	18-19	0.45556517765950844
1103	20-21	0.4324260649458296
1103	22-23	0.3621112464513203
1103	24-25	0.3828113747897035
1103	26-27	0.49980613753446335
1103	28-29	0.4744979226292827
1103	30-31	0.48700198153995444
1103	32-33	0.5749521395907422
1103	34-35	0.5596133990910701
1103	36-37	0.5381683453380077
1103	38-39	0.5596164788353875
1103	40-41	0.6113426859802047
1103	42-43	0.6037400877315164
1103	44-45	0.6936316283901718
1103	46-47	0.6600781434789056
1103	48-49	0.6711294757989066
1103	50-51	0.7080346219942797
1103	52-53	0.7389212345311336
1103	54-55	0.78175173523627
1103	56-57	0.7702441962627162
1103	58-59	0.7830522802607405
1103	60-61	0.8050160695473849
1103	62-63	0.7572845992744845
1103	64-65	0.7073741816499819
1103	66-67	0.7154950825894062
1103	68-69	0.7163740778680037
1103	70-71	0.6898727115879453
1103	72-73	0.79785108139464
1103	74-75	0.8284544751546008
1103	76-77	0.8342470474714148
1103	78-79	0.8233930275767669
1103	80-81	0.7749083829097358
1103	82-83	0.766038956005989
1103	84-85	0.803008782047538
1103	86-87	0.7633897978011674
1103	88-89	0.7205576391687565
1103	90-91	0.7955766426908824
1103	92-93	0.8267255732209691
1103	94-95	0.798221183635313
1103	96-97	0.7484195245016529
1103	98-99	0.7227611463704164
1103	100	0.7129461077345773
1104	1	6.164402261949139
1104	2	5.190435613590164
1104	3	4.195085759405302
1104	4	1.8695197248184243
1104	5	2.871870545451131
1104	6	1.3407634409469438
1104	7	0.7338166094535339
1104	8	0.5301114664994486
1104	9	0.4565265918122421
1104	10-11	0.4546769190388602
1104	12-13	0.4221151611804217
1104	14-15	0.6007062887358572
1104	16-17	0.5176497015942445
1104	18-19	0.5233249478917941
1104	20-21	0.44724153837249503
1104	22-23	0.5088864633267178
1104	24-25	0.5051351467255927
1104	26-27	0.5246323737763348
1104	28-29	0.48497554519420305
1104	30-31	0.583112796275735
1104	32-33	0.6649211930667391
1104	34-35	0.6711148063250434
1104	36-37	0.6367777001508728
1104	38-39	0.7091766633345102
1104	40-41	0.6916415681512547
1104	42-43	0.7446967776910896
1104	44-45	0.8432017263696565
1104	46-47	0.8221052670355888
1104	48-49	0.914337815581149
1104	50-51	0.8605163674693372
1104	52-53	0.881756923499502
1104	54-55	1.0126400887286167
1104	56-57	0.9862546797167369
1104	58-59	1.0432855989948422
1104	60-61	0.9407156305825879
1104	62-63	0.9766587026018954
1104	64-65	0.9527339181832986
1104	66-67	0.9688964011303227
1104	68-69	0.9783613622686786
1104	70-71	1.0073006718012039
1104	72-73	0.9585517538373267
1104	74-75	0.9645524277499078
1104	76-77	0.9731390236033164
1104	78-79	0.9251180966486849
1104	80-81	0.9866578192477675
1104	82-83	0.9891312319160548
1104	84-85	0.951230372276811
1104	86-87	0.8709974369301747
1104	88-89	0.8634252639510009
1104	90-91	0.9452262200267327
1104	92-93	0.9934032167517017
1104	94-95	1.0763508385117326
1104	96-97	1.0545874861638573
1104	98-99	1.0190339796197776
1104	100	1.0087086540157806
1105	1	1.5747630607681096
1105	2	3.1521255466974587
1105	3	3.3373272599217287
1105	4	1.469521211923869
1105	5	2.685186560765615
1105	6	1.239957294604956
1105	7	0.7820134941028201
1105	8	0.5400103838866883
1105	9	0.4465008648880655
1105	10-11	0.47786991693528336
1105	12-13	0.4097356843170843
1105	14-15	0.5619563766102615
1105	16-17	0.5006993357663063
1105	18-19	0.49133257809462805
1105	20-21	0.46508518140229427
1105	22-23	0.45626429085493214
1105	24-25	0.5135193796225295
1105	26-27	0.5042018432311863
1105	28-29	0.5113138724979933
1105	30-31	0.5674499257836985
1105	32-33	0.5843552953687734
1105	34-35	0.5734384085811115
1105	36-37	0.45199721043353236
1105	38-39	0.5327357933507884
1105	40-41	0.5437674867768436
1105	42-43	0.6001849741296041
1105	44-45	0.6393053411174137
1105	46-47	0.6578259791698216
1105	48-49	0.629878878618193
1105	50-51	0.7503294923916073
1105	52-53	0.8590184729011412
1105	54-55	0.8119228645325975
1105	56-57	0.8098844472416005
1105	58-59	0.8077671669988291
1105	60-61	0.8396441035145656
1105	62-63	0.8607595513335369
1105	64-65	0.8457110627245328
1105	66-67	0.7937593170466712
1105	68-69	0.8023873082029311
1105	70-71	0.8863104826413846
1105	72-73	0.8983748829234912
1105	74-75	0.9209016244425925
1105	76-77	0.8316287057953673
1105	78-79	0.9237010203520661
1105	80-81	0.9031668865352316
1105	82-83	0.8446688070148802
1105	84-85	0.820468751223725
1105	86-87	0.9358138064461343
1105	88-89	0.8586404007917423
1105	90-91	0.8063772801977223
1105	92-93	0.8881269666367899
1105	94-95	0.974867991597069
1105	96-97	0.9769428381211611
1105	98-99	0.9645527901517283
1105	100	1.0029768088694233
1106	1	-0.768120703354235
1106	2	1.8051463287813974
1106	3	2.728044139059307
1106	4	1.1842589637941074
1106	5	2.4327176287113303
1106	6	1.0916435337833335
1106	7	0.5810254230591383
1106	8	0.42150289083584624
1106	9	0.33884160166148547
1106	10-11	0.42657063015387564
1106	12-13	0.338143090109881
1106	14-15	0.43648730408506253
1106	16-17	0.3667771663417767
1106	18-19	0.3463190188453069
1106	20-21	0.3812272618526862
1106	22-23	0.3594705914253211
1106	24-25	0.36786626251753063
1106	26-27	0.3119302693549031
1106	28-29	0.4217100350816594
1106	30-31	0.37978209590712453
1106	32-33	0.3780048335792898
1106	34-35	0.43485132457051634
1106	36-37	0.395472578546908
1106	38-39	0.4759715651346781
1106	40-41	0.39496678317669875
1106	42-43	0.42107189227381525
1106	44-45	0.5307011168870801
1106	46-47	0.4643505764576972
1106	48-49	0.45041430808207394
1106	50-51	0.443702206019843
1106	52-53	0.4420969172583966
1106	54-55	0.49235955608960325
1106	56-57	0.38338986782744655
1106	58-59	0.43118225025379786
1106	60-61	0.384180027048572
1106	62-63	0.3529453203149302
1106	64-65	0.4213224053012681
1106	66-67	0.40110487342568035
1106	68-69	0.35937573630569375
1106	70-71	0.3376518069015475
1106	72-73	0.3224018796345405
1106	74-75	0.2813846298835898
1106	76-77	0.3768273683461629
1106	78-79	0.42580559299178944
1106	80-81	0.39584918214832143
1106	82-83	0.4085747011883818
1106	84-85	0.4141378723255684
1106	86-87	0.39265235277282073
1106	88-89	0.4087104286575709
1106	90-91	0.43025854698244004
1106	92-93	0.3827065537906904
1106	94-95	0.4153218856515508
1106	96-97	0.4052526754447676
1106	98-99	0.35502221904403797
1106	100	0.24549507181852448
1107	1	-1.1313053741071926
1107	2	1.5646279450481906
1107	3	-0.7047620635456937
1107	4	-0.14435581769743777
1107	5	1.9005323784590402
1107	6	0.7440458252111739
1107	7	0.3074978884109072
1107	8	0.29618539802289945
1107	9	0.2736780867763997
1107	10-11	0.29247053214624685
1107	12-13	0.25609905920732245
1107	14-15	0.3290499527298394
1107	16-17	0.22315410078596898
1107	18-19	0.2051611873268584
1107	20-21	0.16856245446753348
1107	22-23	0.19827802773744452
1107	24-25	0.18686908763125842
1107	26-27	0.21136900383418578
1107	28-29	0.22779200189685866
1107	30-31	0.21351041028500362
1107	32-33	0.2103019139753357
1107	34-35	0.18627380138291016
1107	36-37	0.23541030744151215
1107	38-39	0.22267396395957206
1107	40-41	0.2500131729191395
1107	42-43	0.2340357588119346
1107	44-45	0.31652037443318193
1107	46-47	0.3731698745556713
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2208	100	0.08204075618655793
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2209	7	0.08252204763485338
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2210	4	-1.0092869541901308
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2214	4	-2.0221304326232072
2214	5	-3.7632388382313096
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2214	9	-0.851683022999346
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2214	96-97	-1.9768059628951171
2214	98-99	-2.032109384879753
2214	100	-2.0537586295321724
2215	1	-2.362261393576304
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2215	4	-1.7383907941015764
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2215	14-15	-0.43144768095199737
2215	16-17	-0.5365658961172883
2215	18-19	-0.49543948049323205
2215	20-21	-0.553185667288453
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2215	24-25	-0.5260508665234269
2215	26-27	-0.4973401746528552
2215	28-29	-0.5189157275148233
2215	30-31	-0.6794041413936398
2215	32-33	-0.6608974609531373
2215	34-35	-0.6527905183763494
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2215	96-97	-0.8742592467479149
2215	98-99	-0.8370579354660421
2215	100	-0.8661307517856827
2216	1	3.0211289263082044
2216	2	-1.66930776166463
2216	3	-2.102715286708314
2216	4	-0.7814792752200361
2216	5	-2.4828441770595404
2216	6	-1.0996475047859136
2216	7	-0.5959134970808293
2216	8	-0.4782992677030862
2216	9	-0.4839684853001742
2216	10-11	-0.35404558201879155
2216	12-13	-0.3674410097360763
2216	14-15	-0.4388787272250667
2216	16-17	-0.5109862162550698
2216	18-19	-0.563359218101823
2216	20-21	-0.5154762417128893
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2216	24-25	-0.579921678011921
2216	26-27	-0.5824644686528018
2216	28-29	-0.6007495230724089
2216	30-31	-0.5813472244926956
2216	32-33	-0.5743268166251951
2216	34-35	-0.6520242075590374
2216	36-37	-0.5962157424090293
2216	38-39	-0.579812221708373
2216	40-41	-0.5445236274181511
2216	42-43	-0.5576269310124218
2216	44-45	-0.5569932180555597
2216	46-47	-0.5441462713667846
2216	48-49	-0.5410340931915982
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2216	54-55	-0.38346847440357124
2216	56-57	-0.21843696675839652
2216	58-59	-0.20669036346814096
2216	60-61	-0.13402187951317046
2216	62-63	-0.07324923363132285
2216	64-65	-0.06430148430122529
2216	66-67	-0.20384214738704998
2216	68-69	-0.16596962500103984
2216	70-71	-0.21607174656731587
2216	72-73	-0.17154660776414232
2216	74-75	-0.2023137504739978
2216	76-77	-0.1193886676393241
2216	78-79	-0.0974819956554569
2216	80-81	-0.13194459027494077
2216	82-83	-0.04772792734705078
2216	84-85	0.06557333023996392
2216	86-87	0.02790245981390882
2216	88-89	0.003767221952621469
2216	90-91	0.08745392280739495
2216	92-93	0.06963105414256887
2216	94-95	0.06455561909709218
2216	96-97	0.016537266941522688
2216	98-99	-0.08809651536118679
2216	100	-0.029991780582886207
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	969.0
3	236.0
4	244.0
5	352.0
6	784.0
7	1200.0
8	1564.0
9	2121.0
10	2194.0
11	3631.0
12	3380.0
13	4714.0
14	4963.0
15	5494.0
16	6692.0
17	8019.0
18	11633.0
19	20231.0
20	41643.0
21	49933.0
22	22092.0
23	13803.0
24	13527.0
25	14586.0
26	15493.0
27	17325.0
28	20407.0
29	24304.0
30	29312.0
31	36275.0
32	47082.0
33	65041.0
34	96279.0
35	166074.0
36	286247.0
37	498211.0
38	547493.0
39	140976.0
40	382.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.495790566151525	38.43519596167989	25.540772505146464	21.528240967022125
2	16.275018159066228	37.346187315262966	25.86228595674906	20.516508568921747
3	20.034132975672726	24.877473674369437	18.941599080917715	36.14679426904012
4	31.146124825048787	26.267671533089487	21.885104359465075	20.701099282396648
5	18.87295431040411	35.918558507700034	21.983665496406292	23.224821685489566
6	35.582287903824685	22.709884545743872	23.771376838304707	17.936450712126742
7	20.682863508652545	22.011904692343755	37.2271316954905	20.0781001035132
8	34.28985314435756	22.29105409397071	23.752464149047196	19.666628612624535
9	33.95747955194511	24.102411517610182	23.530432296915016	18.40967663352969
10-11	18.454892026899113	39.16095781125135	23.469553320454885	18.914596841394648
12-13	18.352011275981997	23.977777038670396	30.820560509073193	26.849651176274413
14-15	18.595032778912906	31.263433151782593	23.393774838127992	26.74775923117651
16-17	26.449454493807828	23.586075097105226	30.910002489992838	19.054467919094108
18-19	18.809423858805722	23.769970506619156	31.060256028794026	26.360349605781096
20-21	26.505119766857565	31.476723061558552	23.34107598253589	18.677081189047986
22-23	26.092585484510355	24.004834361541565	31.090010094808502	18.812570059139578
24-25	18.673979934433184	31.365662189773413	23.36842545258092	26.591932423212487
26-27	18.99545845879746	26.83581392558021	35.36876060578752	18.799967009834802
28-29	25.614632708078457	23.725429299035554	31.940697719364326	18.719240273521667
30-31	24.023981237859036	24.498765341097556	32.21972074325837	19.25753267778504
32-33	20.67745783417367	25.81225903476372	33.10243219264095	20.407850938421667
34-35	20.392726703959628	25.57687830407217	33.50159512356927	20.528799868398934
36-37	20.473808781135023	25.688231323031175	33.491257608186594	20.346702287647208
38-39	20.945559048337323	25.463525200615216	33.168547345370996	20.422368405676465
40-41	20.455515873479598	26.14656529309553	32.98932629063879	20.408592542786078
42-43	20.589143468151924	25.78555533731765	33.18722130657187	20.43807988795856
44-45	20.60044334457276	25.972737724649942	32.851432824577756	20.57538610619954
46-47	20.771551697015514	25.720457403593677	32.95534732703314	20.552643572357667
48-49	20.50884396913847	26.008424625579686	32.80745343848234	20.675277966799495
50-51	20.818789647742424	26.170453942773314	32.39527872188758	20.61547768759669
52-53	20.888645682226525	26.270162400374847	32.08118108546329	20.760010831935332
54-55	21.321867081126125	26.882214349729832	30.57711202181126	21.218806547332786
56-57	21.676286548735092	27.588064394630607	29.22727971428905	21.508369342345247
58-59	22.088708466784666	28.224113737838813	28.28811644177327	21.39906135360325
60-61	21.921847484792618	28.394053501586136	28.021678219214653	21.662420794406597
62-63	21.503772071167084	28.521037976902385	27.924990055755188	22.050199896175343
64-65	21.601923856558432	28.317488469175778	28.512125905543872	21.568461768721914
66-67	21.582062698842716	28.037415521692942	28.750142421779262	21.63037935768508
68-69	21.66770191639557	28.021004033428827	28.65105312314318	21.660240927032422
70-71	21.083430041538833	28.488911441651915	28.86189349123064	21.56576502557861
72-73	21.14788953483538	28.19730590199427	28.87767340606917	21.77713115710118
74-75	21.258528473189806	28.424490988359025	28.451638275877567	21.8653422625736
76-77	21.5337014250313	28.214635409210803	28.454601412154307	21.797061753603593
78-79	21.572532890227475	28.384341595423795	28.02747178413781	22.015653730210918
80-81	21.68073855502644	28.73993487303838	27.803459504648554	21.775867067286626
82-83	21.574306087730378	28.86218838277034	27.69722929499727	21.866276234502006
84-85	21.626710258472016	28.986130956099714	27.375282205850827	22.011876579577443
86-87	21.61867585357577	29.06569044521002	27.35307799658862	21.96255570462559
88-89	21.958658028698743	28.786002644606107	27.280118800524605	21.975220526170546
90-91	21.714819669788074	29.083787641222354	27.22168524536671	21.97970744362286
92-93	21.600418174970088	29.138107407683737	27.158877723373482	22.10259669397269
94-95	21.528110188974363	29.360332238904586	26.92602605504505	22.18553151707601
96-97	21.585711418787923	29.10067345159731	26.951300552411446	22.362314577203325
98-99	21.8776040566427	29.097228901691498	26.546613007181463	22.47855403448434
100	21.68356775522202	29.48551534312011	26.416936266071467	22.4139806355864
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12151.0
1	6775.5
2	1106.0
3	727.5
4	649.5
5	619.0
6	596.0
7	686.5
8	735.0
9	694.5
10	699.0
11	1084.0
12	7867.5
13	9325.0
14	4279.5
15	4383.5
16	4997.0
17	5958.5
18	6944.5
19	8001.0
20	9343.0
21	10760.5
22	12393.5
23	14310.5
24	16480.5
25	18840.0
26	21457.0
27	24414.0
28	27385.5
29	30459.5
30	33713.5
31	36918.0
32	40124.5
33	43616.5
34	47230.5
35	50490.5
36	53612.0
37	56778.5
38	59445.0
39	61194.0
40	62853.0
41	64440.0
42	65524.5
43	67481.5
44	69894.5
45	71699.0
46	73020.5
47	74951.0
48	76241.0
49	76991.5
50	81058.5
51	79741.0
52	72455.5
53	68181.5
54	65839.0
55	63881.0
56	58559.5
57	52262.5
58	46952.0
59	40375.5
60	33301.5
61	26907.5
62	21607.5
63	16825.5
64	12935.5
65	9731.5
66	7314.5
67	5640.5
68	4310.0
69	3279.5
70	2500.0
71	1922.5
72	1463.5
73	1087.5
74	835.5
75	615.5
76	430.5
77	277.5
78	147.0
79	80.5
80	49.0
81	30.5
82	16.0
83	6.5
84	5.0
85	5.5
86	4.0
87	1.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1344775914128507
2	0.005483377724721853
3	0.0035057660862975785
4	0.0
5	0.0020225573574793724
6	0.013573607154639343
7	0.003101254614801704
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	2.247285952754858E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	6.741857858264574E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	1.5731001669284006E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	4.494571905509716E-5
64-65	0.0
66-67	2.247285952754858E-5
68-69	0.0
70-71	0.0
72-73	2.472014548030344E-4
74-75	2.6967431433058296E-4
76-77	3.370928929132287E-4
78-79	2.247285952754858E-4
80-81	9.438601001570404E-4
82-83	9.438601001570404E-4
84-85	0.0010337515382672347
86-87	4.494571905509716E-5
88-89	0.0
90-91	6.741857858264574E-5
92-93	0.0
94-95	4.494571905509716E-5
96-97	0.0011011701168498803
98-99	5.393486286611659E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2224906.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.10606515361556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.42283904997274	71.26361449469321
2	5.3110961321687435	8.190354488082374
3	0.9743864356970331	2.25393311986964
4	0.3797287141979985	1.1711754791059812
5	0.2002004959337685	0.771833624162765
6	0.1294214943115995	0.5987509303601085
7	0.09343307257541376	0.504297960705164
8	0.07562548829208418	0.4664947062018746
9	0.05494544621228293	0.38129644399848894
>10	0.33853381224691115	4.13582178274786
>50	0.00947363662047475	0.49622676391909215
>100	0.009384261160103007	1.5076659888831674
>500	1.1649507634583614E-4	0.05793697630684359
>1k	6.407229199020944E-4	1.2213923028407763
>5k	0.0	0.0
>10k+	1.7474261451875305E-4	6.979204938122644
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	132176	5.940745361826522	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12706	0.5710803063140645	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10514	0.4725592901452916	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	4846	0.21780695454100082	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4212	0.18931136866006923	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3683	0.16553508327992283	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3592	0.16144502284590898	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2964	0.13321911127930797	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0027416888623609265	0.0	0.0	4.494571905509716E-5	4.494571905509716E-5
2	0.002786634581416024	0.0	0.0	8.989143811019432E-5	4.494571905509716E-5
3	0.002786634581416024	0.0	0.0	8.989143811019432E-5	4.494571905509716E-5
4	0.002786634581416024	4.494571905509716E-5	0.0	1.3483715716529148E-4	4.494571905509716E-5
5	0.002786634581416024	8.989143811019432E-5	0.0	1.3483715716529148E-4	1.3483715716529148E-4
6	0.002786634581416024	1.3483715716529148E-4	0.0	1.3483715716529148E-4	1.3483715716529148E-4
7	0.002786634581416024	1.7978287622038864E-4	0.0	1.3483715716529148E-4	1.7978287622038864E-4
8	0.002831580300471121	1.7978287622038864E-4	0.0	1.3483715716529148E-4	1.7978287622038864E-4
9	0.002831580300471121	1.7978287622038864E-4	0.0	1.3483715716529148E-4	1.7978287622038864E-4
10-11	0.002831580300471121	1.7978287622038864E-4	0.0	1.3483715716529148E-4	1.7978287622038864E-4
12-13	0.002831580300471121	1.7978287622038864E-4	0.0	1.3483715716529148E-4	1.7978287622038864E-4
14-15	0.002831580300471121	1.7978287622038864E-4	0.0	1.5731001669284006E-4	1.7978287622038864E-4
16-17	0.002831580300471121	1.7978287622038864E-4	0.0	1.7978287622038864E-4	1.7978287622038864E-4
18-19	0.002831580300471121	1.7978287622038864E-4	0.0	1.7978287622038864E-4	1.7978287622038864E-4
20-21	0.002831580300471121	2.0225573574793722E-4	0.0	2.247285952754858E-4	2.247285952754858E-4
22-23	0.002831580300471121	2.247285952754858E-4	0.0	2.472014548030344E-4	2.247285952754858E-4
24-25	0.002831580300471121	2.472014548030344E-4	0.0	2.6967431433058296E-4	2.247285952754858E-4
26-27	0.0028540531599986693	3.146200333856801E-4	0.0	2.6967431433058296E-4	2.247285952754858E-4
28-29	0.002943944598108864	3.595657524407773E-4	0.0	2.6967431433058296E-4	2.6967431433058296E-4
30-31	0.002988890317163961	4.0451147149587444E-4	0.0	3.370928929132287E-4	2.6967431433058296E-4
32-33	0.003146200333856801	4.494571905509716E-4	0.0	3.595657524407773E-4	2.6967431433058296E-4
34-35	0.0037304946815730645	4.494571905509716E-4	0.0	3.595657524407773E-4	2.6967431433058296E-4
36-37	0.004629409062675008	4.944029096060688E-4	0.0	4.0451147149587444E-4	2.6967431433058296E-4
38-39	0.005685633460469791	5.393486286611659E-4	0.0	4.0451147149587444E-4	2.6967431433058296E-4
40-41	0.008045283710862392	5.393486286611659E-4	0.0	4.0451147149587444E-4	2.6967431433058296E-4
42-43	0.011483631218577324	5.618214881887145E-4	0.0	4.26984331023423E-4	2.6967431433058296E-4
44-45	0.015056815883457548	5.842943477162631E-4	0.0	4.719300500785202E-4	2.9214717385813154E-4
46-47	0.01880978342455816	6.741857858264574E-4	0.0	5.393486286611659E-4	3.370928929132287E-4
48-49	0.023484138206288266	7.640772239366517E-4	0.0	5.842943477162631E-4	3.595657524407773E-4
50-51	0.028900097352427472	8.53968662046846E-4	0.0	5.842943477162631E-4	3.595657524407773E-4
52-53	0.035619482351164494	8.53968662046846E-4	0.0	5.842943477162631E-4	3.595657524407773E-4
54-55	0.044743463319349225	8.989143811019432E-4	0.0	5.842943477162631E-4	3.595657524407773E-4
56-57	0.054406792916195115	0.001011278678739686	0.0	5.842943477162631E-4	3.8203861196832586E-4
58-59	0.06544096694422147	0.0011685886954325262	0.0	5.842943477162631E-4	4.0451147149587444E-4
60-61	0.08085734858011978	0.0012135344144876233	0.0	5.842943477162631E-4	4.0451147149587444E-4
62-63	0.09762210178767103	0.001280952993070269	0.0	5.842943477162631E-4	4.0451147149587444E-4
64-65	0.11789262108151985	0.001393317290708012	0.0	6.292400667713602E-4	4.0451147149587444E-4
66-67	0.14317458805001199	0.001393317290708012	0.0	6.292400667713602E-4	4.0451147149587444E-4
68-69	0.17331069267645466	0.0014607358692906576	0.0	6.292400667713602E-4	4.0451147149587444E-4
70-71	0.21151455387328721	0.0015731001669284006	0.0	6.292400667713602E-4	4.0451147149587444E-4
72-73	0.2557411414235028	0.0015731001669284006	0.0	6.96658645354006E-4	4.0451147149587444E-4
74-75	0.3109569572826897	0.0015731001669284006	0.0	7.191315048815546E-4	4.494571905509716E-4
76-77	0.3752518083910062	0.0016405187455110462	0.0	7.640772239366517E-4	4.944029096060688E-4
78-79	0.45069319782498674	0.001662991605038595	0.0	7.640772239366517E-4	4.944029096060688E-4
80-81	0.5456410293288796	0.001662991605038595	0.0	7.640772239366517E-4	4.944029096060688E-4
82-83	0.6589941327858345	0.0017304101836212405	0.0	8.090229429917489E-4	4.944029096060688E-4
84-85	0.7968875988468727	0.0017528830431487892	0.0	8.090229429917489E-4	5.842943477162631E-4
86-87	0.9629170850364015	0.0017978287622038864	0.0	8.090229429917489E-4	6.292400667713602E-4
88	1.1025184884215333	0.0017978287622038864	0.0	8.53968662046846E-4	7.191315048815546E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	2975	0.0	86.70109	1
AACGAGT	32505	0.0	81.97359	1
ACGAGTG	36420	0.0	80.39007	2
CGAGTGG	37875	0.0	79.532394	3
GTGGAAT	40240	0.0	79.25862	6
TGGAATC	40565	0.0	78.56491	7
GAATCAC	40680	0.0	78.36591	9
GGAATCA	40850	0.0	78.00527	8
GAGTGGA	39725	0.0	77.970406	4
AGTGGAA	40250	0.0	76.90668	5
ATCGAGT	895	0.0	71.523186	1
AACGTGT	1370	0.0	61.841908	1
ACGTGTG	1480	0.0	60.98643	2
CGTGTGG	1570	0.0	60.781376	3
TCGAGTG	1130	0.0	59.90702	2
TGAGTGG	2210	0.0	43.817654	3
ATGAGTG	2005	0.0	43.610622	2
AGTACTT	22230	0.0	41.248116	20-21
GAGTACT	21830	0.0	41.045864	20-21
GTGTGGA	2555	0.0	40.47678	4
>>END_MODULE