FastQCFastQC Report
Thu 26 May 2016
SRR2132756_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132756_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3153084
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2575218.167273691408159No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA734892.330702258487246No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG213040.6756559609575895No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT204210.6476516324969458No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT88090.27937726999978435No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT73170.23205851794623927No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC68950.21867479585066557No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66300.2102703258143456No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64760.20538621869889925No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT61370.19463484004866347No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA40980.1299679932409032No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA39630.12568647076957035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT51300.087.53921
AACGTGT23250.073.29211
CGTGTGG26700.071.835883
GTGGAAT888750.071.4468846
TGGAATC894950.070.948927
AACGAGT758900.070.662171
ACGAGTG825400.070.5768052
GGAATCA903200.070.346588
GAATCAC904950.070.2053459
CGAGTGG855850.069.983943
ACGTGTG25800.069.973182
AGTGGAA880900.069.78365
ATCGAGT16850.069.561831
GAGTGGA888050.069.2270364
TCGAGTG18900.063.1817932
ATGAGTG28550.058.4577832
TGAGTGG33150.054.1716883
GCGAGTG10950.050.662662
AATGAGT31050.049.7260321
GTGTGGA42900.047.6654624
Produced by FastQC (version 0.11.5)