##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132756_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3153084
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.930262561986932	31.0	25.0	33.0	16.0	34.0
2	28.189497330232875	31.0	25.0	34.0	16.0	34.0
3	29.2354536066911	31.0	28.0	34.0	16.0	34.0
4	34.74871649470804	35.0	35.0	37.0	32.0	37.0
5	33.802653529052826	35.0	35.0	37.0	28.0	37.0
6	35.249170653239815	37.0	35.0	37.0	32.0	37.0
7	35.62917194721105	37.0	35.0	37.0	33.0	37.0
8	35.917444318007384	37.0	35.0	37.0	35.0	37.0
9	37.885712844947996	39.0	38.0	39.0	35.0	39.0
10-11	37.882052777534625	39.0	38.0	39.0	35.0	39.0
12-13	37.86868205858138	39.0	38.0	39.0	35.0	39.0
14-15	39.29369769406714	41.0	39.0	41.0	36.0	41.0
16-17	39.270680387836165	40.5	39.0	41.0	36.0	41.0
18-19	39.152084276854026	40.5	39.0	41.0	35.5	41.0
20-21	39.07217140425057	40.0	39.0	41.0	35.0	41.0
22-23	38.936077028077904	40.0	39.0	41.0	35.0	41.0
24-25	38.83040001471575	40.0	39.0	41.0	35.0	41.0
26-27	38.64452865829138	40.0	38.5	41.0	35.0	41.0
28-29	38.44665318145663	40.0	38.0	41.0	34.5	41.0
30-31	38.29416200139292	40.0	38.0	41.0	34.0	41.0
32-33	38.12323379269312	40.0	38.0	41.0	34.0	41.0
34-35	38.02674778090276	40.0	38.0	41.0	34.0	41.0
36-37	37.83574240331053	40.0	38.0	41.0	33.0	41.0
38-39	37.296186685797146	40.0	36.0	41.0	33.0	41.0
40-41	37.02215846453821	40.0	35.0	41.0	33.0	41.0
42-43	36.845468753766156	40.0	35.0	41.0	33.0	41.0
44-45	36.68565331592815	39.0	35.0	41.0	32.5	41.0
46-47	36.53113253563812	39.0	35.0	41.0	32.0	41.0
48-49	36.34406980594237	39.0	35.0	41.0	31.0	41.0
50-51	36.024747041309396	39.0	35.0	41.0	30.5	41.0
52-53	35.49157507380076	39.0	35.0	41.0	29.0	41.0
54-55	34.49339725804958	39.0	35.0	40.5	22.0	41.0
56-57	33.663298852805696	38.0	34.0	40.0	7.0	41.0
58-59	33.27360482625899	38.0	34.0	40.0	2.0	41.0
60-61	33.00741860984357	37.5	34.0	40.0	2.0	41.0
62-63	32.733102416554715	37.0	34.0	40.0	2.0	41.0
64-65	32.44096525814092	36.5	33.5	40.0	2.0	41.0
66-67	32.118067580819286	36.0	33.0	39.5	2.0	41.0
68-69	31.798696609414783	35.0	33.0	39.0	2.0	41.0
70-71	31.47156165202069	35.0	33.0	39.0	2.0	40.5
72-73	31.14594536650467	35.0	33.0	37.5	2.0	40.0
74-75	30.810067381649205	35.0	33.0	37.0	2.0	39.5
76-77	30.48058488134157	35.0	32.0	37.0	2.0	39.0
78-79	30.146205112201258	35.0	32.0	36.0	2.0	39.0
80-81	29.83253237148138	35.0	32.0	36.0	2.0	38.5
82-83	29.533066832345728	35.0	31.5	35.5	2.0	37.0
84-85	29.255437374963684	35.0	31.0	35.0	2.0	37.0
86-87	29.04958447031541	35.0	31.0	35.0	2.0	36.5
88-89	28.81969652568723	35.0	31.0	35.0	2.0	36.0
90-91	28.615046253128682	35.0	31.0	35.0	2.0	36.0
92-93	28.43629950867151	35.0	31.0	35.0	2.0	36.0
94-95	28.232969372208288	34.5	30.5	35.0	2.0	35.0
96-97	28.024942405594015	34.0	30.0	35.0	2.0	35.0
98-99	27.848880968600902	34.0	30.0	35.0	2.0	35.0
100	27.74650374046489	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.21092056625919398
1101	2	2.3347438779838185
1101	3	1.1551802967528317
1101	4	0.651567083781238
1101	5	2.243720515545064
1101	6	0.9570405247982308
1101	7	0.5181234222967319
1101	8	0.37857888674754037
1101	9	0.28760263873986247
1101	10-11	0.445671182975623
1101	12-13	0.31314535713563885
1101	14-15	0.4287230383164271
1101	16-17	0.3640112852128041
1101	18-19	0.3603671233039307
1101	20-21	0.31335835844947724
1101	22-23	0.3382935748801472
1101	24-25	0.36683670819945746
1101	26-27	0.31852083948721344
1101	28-29	0.3774586048617863
1101	30-31	0.35825436837763647
1101	32-33	0.44632484796579064
1101	34-35	0.4305698787712089
1101	36-37	0.4295425204253718
1101	38-39	0.4548358031658992
1101	40-41	0.41723026265061236
1101	42-43	0.45506832849915213
1101	44-45	0.5100748495062604
1101	46-47	0.44213264378824846
1101	48-49	0.34292732808475535
1101	50-51	0.47131590262306844
1101	52-53	0.5306400436965433
1101	54-55	0.5182109767574801
1101	56-57	0.42398590874893927
1101	58-59	0.4373908175950305
1101	60-61	0.4340931056092785
1101	62-63	0.48818712995642954
1101	64-65	0.46850994985572925
1101	66-67	0.4256790517533702
1101	68-69	0.537866304440584
1101	70-71	0.474953877651604
1101	72-73	0.4627405082497127
1101	74-75	0.5221156948082779
1101	76-77	0.43931317343681187
1101	78-79	0.46440204144355945
1101	80-81	0.45068773515099636
1101	82-83	0.49774255266333256
1101	84-85	0.4601951446306991
1101	86-87	0.5062946557516419
1101	88-89	0.4394656731922453
1101	90-91	0.3669819206917744
1101	92-93	0.31418715982143297
1101	94-95	0.37201646744405537
1101	96-97	0.3817942708304827
1101	98-99	0.4150748794910868
1101	100	0.4194038138757925
1102	1	1.4981744189923205
1102	2	2.992959151894631
1102	3	3.18205515684825
1102	4	1.4360699423054086
1102	5	2.641273673118164
1102	6	1.2036235385478804
1102	7	0.7222159896405529
1102	8	0.4896286659526794
1102	9	0.41489337386558134
1102	10-11	0.500458933470334
1102	12-13	0.4414743538124668
1102	14-15	0.5930968115498416
1102	16-17	0.48665580086084503
1102	18-19	0.3619717478786697
1102	20-21	0.32114449144685153
1102	22-23	0.2747941243235843
1102	24-25	0.31808003979998034
1102	26-27	0.372715171031615
1102	28-29	0.3139637792499457
1102	30-31	0.3807361090958281
1102	32-33	0.35962740691687856
1102	34-35	0.35544848168023435
1102	36-37	0.3695384874735623
1102	38-39	0.3943329699247542
1102	40-41	0.4229708211342711
1102	42-43	0.5560039702348973
1102	44-45	0.5743480918576864
1102	46-47	0.5693755492772041
1102	48-49	0.49186850865431353
1102	50-51	0.5200670981712747
1102	52-53	0.5652700111184359
1102	54-55	0.6094127671044092
1102	56-57	0.5267977363976044
1102	58-59	0.5278468356039525
1102	60-61	0.5961075789113224
1102	62-63	0.5756518493363671
1102	64-65	0.5630038529452506
1102	66-67	0.5858402326189278
1102	68-69	0.5786303746016088
1102	70-71	0.5583186991503197
1102	72-73	0.5510772609588486
1102	74-75	0.6236453721536357
1102	76-77	0.5324597915847207
1102	78-79	0.5167362972992002
1102	80-81	0.5399126699546919
1102	82-83	0.5512143907581972
1102	84-85	0.5587441221787515
1102	86-87	0.573130117484542
1102	88-89	0.6184453302186803
1102	90-91	0.6419004677068045
1102	92-93	0.6371926832387622
1102	94-95	0.6683676635472615
1102	96-97	0.6442290514015241
1102	98-99	0.7168632377035351
1102	100	0.6965493103204459
1103	1	1.2179689350392096
1103	2	2.9479409980055067
1103	3	3.1616289511753664
1103	4	1.3535785879924234
1103	5	2.583505697892882
1103	6	1.221558718484964
1103	7	0.7079259627793704
1103	8	0.47578221828270273
1103	9	0.3886020655533997
1103	10-11	0.47075196270549924
1103	12-13	0.4442287546930501
1103	14-15	0.552299847602967
1103	16-17	0.41044027066048727
1103	18-19	0.4277416042082933
1103	20-21	0.3741794150937281
1103	22-23	0.3800871946308817
1103	24-25	0.38143536726821026
1103	26-27	0.47302978368018955
1103	28-29	0.5640269276764727
1103	30-31	0.5245558804061119
1103	32-33	0.5016014986063411
1103	34-35	0.5352248643740722
1103	36-37	0.5144471840900309
1103	38-39	0.5320673892056647
1103	40-41	0.5572325903580904
1103	42-43	0.6857457496446102
1103	44-45	0.7400321073993865
1103	46-47	0.6638912881240557
1103	48-49	0.7138685990130398
1103	50-51	0.7302327626775522
1103	52-53	0.670158007372649
1103	54-55	0.6801241161601652
1103	56-57	0.7156577378762066
1103	58-59	0.707663263641436
1103	60-61	0.7414182691698272
1103	62-63	0.6749355510868611
1103	64-65	0.7468083864888726
1103	66-67	0.7786703705391957
1103	68-69	0.6915454086504198
1103	70-71	0.6217571977168994
1103	72-73	0.6866512852899902
1103	74-75	0.6874607076720061
1103	76-77	0.7710129841227342
1103	78-79	0.7758912789399517
1103	80-81	0.7860739643844852
1103	82-83	0.7650709011042736
1103	84-85	0.7499334278130299
1103	86-87	0.7351730871650837
1103	88-89	0.7345881216460981
1103	90-91	0.7792215985051634
1103	92-93	0.7846047272352443
1103	94-95	0.7970241114636707
1103	96-97	0.7569515597597061
1103	98-99	0.7888421759063853
1103	100	0.9228321988424213
1104	1	6.040374248742367
1104	2	5.055114958900912
1104	3	4.0721399365703945
1104	4	1.85427553723504
1104	5	2.8637175749663513
1104	6	1.2872368956981006
1104	7	0.7171827773159976
1104	8	0.5448055266748639
1104	9	0.4822854423006291
1104	10-11	0.44961112209913523
1104	12-13	0.40784482348273343
1104	14-15	0.5282423616756589
1104	16-17	0.40244829514716685
1104	18-19	0.3997231750237944
1104	20-21	0.34362047080858105
1104	22-23	0.342914410329918
1104	24-25	0.36612633952366735
1104	26-27	0.4311976994698057
1104	28-29	0.3703561753935105
1104	30-31	0.4893322176845132
1104	32-33	0.5326903326125105
1104	34-35	0.4992268448808872
1104	36-37	0.520457175009426
1104	38-39	0.5936896933829132
1104	40-41	0.5898227082033074
1104	42-43	0.6545021709509911
1104	44-45	0.6251214047248013
1104	46-47	0.5652078072285178
1104	48-49	0.63628071050465
1104	50-51	0.6962832278827236
1104	52-53	0.763426268653177
1104	54-55	0.8209338298761608
1104	56-57	0.7977052852397577
1104	58-59	0.8246684733088898
1104	60-61	0.8164484721802268
1104	62-63	0.8595296372642451
1104	64-65	0.8410207381408625
1104	66-67	0.8297533581658101
1104	68-69	0.7271531534566549
1104	70-71	0.8000814851594917
1104	72-73	0.8367417395061096
1104	74-75	0.8410640562935612
1104	76-77	0.8197544643686143
1104	78-79	0.808831560298021
1104	80-81	0.7980327239049103
1104	82-83	0.8241088493712745
1104	84-85	0.784733600980708
1104	86-87	0.7855507514618587
1104	88-89	0.7568230824891486
1104	90-91	0.7700386729734596
1104	92-93	0.7706599016329996
1104	94-95	0.7709175075036683
1104	96-97	0.7059020684819828
1104	98-99	0.7441554427687009
1104	100	0.7083649287160405
1105	1	1.6071090501243042
1105	2	3.066280833075382
1105	3	3.248422050717295
1105	4	1.3721095537835097
1105	5	2.590753560743515
1105	6	1.15959344153422
1105	7	0.7038918845784323
1105	8	0.4640583237473592
1105	9	0.39507814371366123
1105	10-11	0.4183814189124533
1105	12-13	0.3681714358097423
1105	14-15	0.4548168620132955
1105	16-17	0.3951745757202261
1105	18-19	0.36058181253497423
1105	20-21	0.3445959086873671
1105	22-23	0.27524465962171973
1105	24-25	0.3367918928718865
1105	26-27	0.36852492262758574
1105	28-29	0.4351831813981448
1105	30-31	0.4226025999580898
1105	32-33	0.4355071590385009
1105	34-35	0.4735234088177762
1105	36-37	0.5442979975574929
1105	38-39	0.4946264639935478
1105	40-41	0.47253437647177776
1105	42-43	0.5477683222408771
1105	44-45	0.5857489492692096
1105	46-47	0.6384955197233708
1105	48-49	0.5078408647254093
1105	50-51	0.559883949111331
1105	52-53	0.6354089608477125
1105	54-55	0.7013649699161206
1105	56-57	0.596904270364071
1105	58-59	0.4448686885006339
1105	60-61	0.43563794459617355
1105	62-63	0.4943385144844612
1105	64-65	0.4574282687314408
1105	66-67	0.37082290040699917
1105	68-69	0.45055868904034924
1105	70-71	0.42415606255146443
1105	72-73	0.4359428961617837
1105	74-75	0.450418382964731
1105	76-77	0.41032070167916856
1105	78-79	0.38429906998585395
1105	80-81	0.37235989942908887
1105	82-83	0.39242256420297394
1105	84-85	0.3935858836104167
1105	86-87	0.4896276965235167
1105	88-89	0.4803032206826039
1105	90-91	0.4536787059246983
1105	92-93	0.5084594678871248
1105	94-95	0.4996926797551424
1105	96-97	0.5312957461034458
1105	98-99	0.549534676204118
1105	100	0.5994451184658551
1106	1	-0.6851636984855816
1106	2	1.8815629316274425
1106	3	2.683876795318639
1106	4	1.181409495888687
1106	5	2.4391112397729344
1106	6	1.1215687786257433
1106	7	0.609892248732578
1106	8	0.4432856057207317
1106	9	0.353771185951274
1106	10-11	0.4093540589873257
1106	12-13	0.3771555168681786
1106	14-15	0.4146684763114621
1106	16-17	0.34648489052864306
1106	18-19	0.25475669756325914
1106	20-21	0.28077611662507707
1106	22-23	0.27562520781595623
1106	24-25	0.26362365223427275
1106	26-27	0.3079413015982482
1106	28-29	0.33297418110085175
1106	30-31	0.36717833904164365
1106	32-33	0.30964968982446095
1106	34-35	0.40647235326862585
1106	36-37	0.3803101398222708
1106	38-39	0.45778818257351617
1106	40-41	0.4076940993163305
1106	42-43	0.4241113540232888
1106	44-45	0.44136460448351755
1106	46-47	0.4157229410995029
1106	48-49	0.4422196476255209
1106	50-51	0.49428670075109693
1106	52-53	0.5639586814020845
1106	54-55	0.4081992968497232
1106	56-57	0.3960671070110564
1106	58-59	0.30601607277856147
1106	60-61	0.28986963624864615
1106	62-63	0.2878280144695182
1106	64-65	0.30794181853734415
1106	66-67	0.3821624969071351
1106	68-69	0.3205521740950914
1106	70-71	0.31950616262273
1106	72-73	0.3962248453537498
1106	74-75	0.4336830462146075
1106	76-77	0.3817456610318857
1106	78-79	0.34173293546788486
1106	80-81	0.3228697152325246
1106	82-83	0.3800374825741244
1106	84-85	0.3405253885030284
1106	86-87	0.3472540587231805
1106	88-89	0.4263261524364168
1106	90-91	0.4726207314467814
1106	92-93	0.4952790081709644
1106	94-95	0.511327902564851
1106	96-97	0.5872417005073629
1106	98-99	0.514085977130577
1106	100	0.504982679685714
1107	1	-0.8418505388150059
1107	2	1.7090322557410609
1107	3	-0.6589920977322983
1107	4	-0.09830973292471867
1107	5	1.831950896594691
1107	6	0.6774192811358901
1107	7	0.291648103252399
1107	8	0.2784660129720251
1107	9	0.27885975496229065
1107	10-11	0.28162954741845425
1107	12-13	0.27732730833049146
1107	14-15	0.3662415316591421
1107	16-17	0.2784622506544423
1107	18-19	0.20486923378361155
1107	20-21	0.20266483610777897
1107	22-23	0.18188015098365184
1107	24-25	0.06740716294194726
1107	26-27	0.08881185001496306
1107	28-29	0.16390913193096424
1107	30-31	0.14339243272075208
1107	32-33	0.19629453750584958
1107	34-35	0.22658591153851404
1107	36-37	0.21747885435424053
1107	38-39	0.2055168177873412
1107	40-41	0.15449162820141282
1107	42-43	0.18295274714770215
1107	44-45	0.26283115990629113
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2216	94-95	0.18123405573525986
2216	96-97	0.15541119228056388
2216	98-99	0.1132542934096108
2216	100	0.05862023277067152
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1564.0
3	425.0
4	453.0
5	1077.0
6	2749.0
7	4740.0
8	5772.0
9	7867.0
10	6351.0
11	7966.0
12	7070.0
13	9962.0
14	10251.0
15	11292.0
16	13464.0
17	15616.0
18	22803.0
19	39426.0
20	78103.0
21	90892.0
22	39421.0
23	24505.0
24	24297.0
25	25128.0
26	26402.0
27	28324.0
28	33595.0
29	38944.0
30	45450.0
31	55723.0
32	70644.0
33	97352.0
34	143775.0
35	272224.0
36	401540.0
37	645526.0
38	664983.0
39	176732.0
40	676.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.711633023514006	43.74448225620623	23.671041483271154	18.87284323700861
2	14.695656531505099	43.55571568940264	23.375203541608123	18.373424237484134
3	18.16668093892425	24.315518384253576	17.997792557493412	39.52000811932876
4	36.003988476044405	25.918370712610255	19.936766670345605	18.14087414099973
5	16.912261894945164	42.19348434199593	20.554325694096455	20.339928068962454
6	39.71504216892502	22.61535196913607	21.99207213425289	15.677533727686027
7	18.67382985080118	22.10040425325423	41.66777144372266	17.557994452221934
8	38.83838806704801	22.607168093206525	21.441769391490997	17.11267444825447
9	38.09746267463854	24.328530416569937	21.50161556114585	16.07239134764567
10-11	16.23735682271706	45.3423537083059	21.6643609875284	16.755928481448638
12-13	16.09389410494614	23.923419103328676	32.08071843312769	27.90196835859749
14-15	16.46357978410978	34.17481107385658	21.589117194467384	27.77249194756626
16-17	27.484186910339208	23.37262502362766	32.194495928430705	16.94869213760242
18-19	16.781157749048234	23.61388088614195	32.41656422727716	27.18839713753265
20-21	27.54766444534938	34.56576164796117	21.629331790716645	16.257242115972804
22-23	26.999486851603066	23.899728012320637	32.53495625235484	16.565828883721462
24-25	16.399547236927404	34.49579839928146	21.623987816372793	27.480666547418338
26-27	16.97590897543769	28.3293684308342	38.16900792018478	16.525714673543323
28-29	26.797700283278214	23.603890667042172	33.18179915282942	16.416609896850197
30-31	23.90686388310619	25.092972467590464	33.67401566212635	17.326147987177
32-33	19.15197945884093	27.11889375608135	34.79201632433516	18.937110460742563
34-35	19.026895572715475	26.734317893211852	35.18799372297091	19.050792811101765
36-37	19.21866020695928	26.80199766324018	35.42100369035522	18.55833843944532
38-39	19.902657207990654	26.412981702993005	34.928406601283065	18.755954487733277
40-41	18.653705388121598	27.90409326234252	34.77752257789516	18.664678771640716
42-43	18.71018251009298	26.827332889637155	35.44425198580996	19.018232614459897
44-45	18.776838802899004	27.562887636358564	34.46628760921054	19.193985951531896
46-47	19.48015022752328	26.796939123727753	34.8890165945468	18.833894054202172
48-49	18.863468274235636	27.10566860889212	34.88930202937822	19.141561087494022
50-51	19.32024329196431	27.83019735598544	33.95911748624521	18.890441865805034
52-53	19.59553833077499	27.393707105433418	33.79278356400726	19.217970999784338
54-55	20.003161983632534	28.440393595603542	31.50729571429115	20.049148706472774
56-57	20.48050416671424	29.25548447171087	29.92381427199529	20.340197089579597
58-59	21.45918091620775	29.759007371830247	28.570028581541116	20.211783130420883
60-61	20.95418326945936	30.10224275661543	28.41513261302268	20.528441360902534
62-63	20.030053135313324	30.199611047223	28.21344322792747	21.556892589536204
64-65	20.46477353600475	30.070036193136623	29.064972579227195	20.400217691631433
66-67	20.59042829179305	29.429694863822213	29.517339214559463	20.462537629825274
68-69	20.774375183154017	29.677785304799997	29.294208463840484	20.253631048205502
70-71	19.311616817059107	30.56553520299491	29.592376860242226	20.530471119703755
72-73	19.442657385129	29.835767971495713	29.744650411289697	20.976924232085594
74-75	19.73114507864701	30.22396225146492	28.905947715817824	21.138944954070247
76-77	20.524163163196178	29.567271707773774	29.09837859301302	20.81018653601703
78-79	20.29775718763206	30.053828448283383	28.477603987856288	21.170810376228268
80-81	20.54138905239869	30.63240868541504	28.12611003420174	20.70009222798453
82-83	20.466536020554084	30.633685447758946	28.068785527142477	20.830993004544492
84-85	20.40624027227096	30.842215382605108	27.473065241415775	21.278479103708158
86-87	20.265708239476766	31.136070813243865	27.606699158680435	20.991521788598934
88-89	21.48737553455601	30.405326975113887	27.30030344894078	20.806994041389316
90-91	20.753221887857958	31.05709446457378	27.24869306422939	20.94099058333887
92-93	20.112071229310732	31.262630491290434	27.24256632554033	21.382731953858507
94-95	20.42911003956761	31.383337710000745	27.02818890965163	21.15936334078001
96-97	20.292742087538457	30.523507646647364	27.49481969592285	21.68893056989133
98-99	21.10764657927124	30.660847775504678	26.5332588449297	21.69824680029438
100	20.22480213023186	31.598333568024195	26.384041782584923	21.79282251915902
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	74260.0
1	40342.5
2	4752.0
3	2709.5
4	2259.5
5	2081.5
6	2072.0
7	2376.5
8	2410.0
9	2247.5
10	2305.5
11	3457.5
12	23418.0
13	26569.5
14	9891.0
15	9458.5
16	10699.5
17	12132.0
18	13553.0
19	15162.0
20	16955.5
21	18903.0
22	21270.5
23	24183.0
24	27285.0
25	30745.5
26	35679.5
27	40521.5
28	43871.5
29	46865.0
30	50339.5
31	54013.0
32	58060.5
33	62712.5
34	67618.5
35	71845.5
36	75491.0
37	79019.0
38	81474.0
39	82958.5
40	84607.5
41	85973.0
42	86555.0
43	86573.0
44	87095.0
45	88384.5
46	90516.0
47	94107.5
48	96126.5
49	102456.0
50	125939.0
51	127510.0
52	101080.0
53	88164.5
54	84929.0
55	84683.0
56	77320.5
57	65350.0
58	57948.5
59	48299.0
60	38227.0
61	31187.0
62	25203.0
63	19659.5
64	14766.5
65	10913.5
66	8323.5
67	6587.5
68	5227.5
69	3979.5
70	2920.5
71	2225.0
72	1790.5
73	1267.5
74	827.0
75	547.0
76	373.0
77	246.0
78	166.0
79	93.5
80	45.5
81	20.5
82	12.0
83	8.0
84	5.0
85	2.5
86	1.0
87	0.0
88	0.0
89	1.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1316806022294363
2	0.005264686890675922
3	0.003615507864681055
4	0.0
5	0.002029758801224452
6	0.012907997376536749
7	0.003488647939604527
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	3.171498126913206E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	8.087320223628676E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	1.2685992507652824E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	6.342996253826412E-5
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	4.598672284024149E-4
74-75	2.537198501530565E-4
76-77	6.342996253826412E-4
78-79	3.647222845950187E-4
80-81	8.880194755356977E-4
82-83	0.0011734543069578862
84-85	0.001538176591552905
86-87	4.757247190369809E-5
88-89	0.0
90-91	7.928745317283016E-5
92-93	0.0
94-95	0.0
96-97	0.0011258818350541881
98-99	6.501571160172073E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3153084.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.286373322023955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.56440333497856	53.66504712507777
2	8.410429278115888	10.308894170741818
3	1.8606674534520389	3.4210068054120386
4	0.6878927847122048	1.6863381603759535
5	0.34923709474146897	1.070173748311236
6	0.2080647584054125	0.7650920675274498
7	0.1365669515467961	0.5858785223163392
8	0.09119691145522915	0.447130236900859
9	0.07197589356185687	0.3970027334716398
>10	0.5505121859134656	7.596101741901
>50	0.043823683968881026	1.7993837510382207
>100	0.020458505312429147	2.377531436451642
>500	0.0028523262062360795	1.24759400509432
>1k	0.001400232864803121	1.4427441130857441
>5k	3.1116285884513805E-4	1.343293838156894
>10k+	2.0744190589675867E-4	11.846787544137086
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	257521	8.167273691408159	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	73489	2.330702258487246	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	21304	0.6756559609575895	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20421	0.6476516324969458	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8809	0.27937726999978435	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7317	0.23205851794623927	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	6895	0.21867479585066557	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6630	0.2102703258143456	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6476	0.20538621869889925	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6137	0.19463484004866347	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA	4098	0.1299679932409032	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	3963	0.12568647076957035	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0038375127335649795	0.0	0.0	0.0	0.0
2	0.0038692277148341114	0.0	0.0	3.171498126913206E-5	0.0
3	0.0038692277148341114	0.0	0.0	6.342996253826412E-5	0.0
4	0.0039009426961032437	0.0	0.0	6.342996253826412E-5	0.0
5	0.0039009426961032437	0.0	0.0	9.514494380739619E-5	0.0
6	0.0039009426961032437	0.0	0.0	9.514494380739619E-5	0.0
7	0.003932657677372376	0.0	0.0	9.514494380739619E-5	0.0
8	0.003932657677372376	0.0	0.0	1.2685992507652824E-4	0.0
9	0.003932657677372376	0.0	0.0	1.2685992507652824E-4	0.0
10-11	0.0039643726586415075	0.0	0.0	1.585749063456603E-4	0.0
12-13	0.00399608763991064	0.0	0.0	2.2200486888392443E-4	0.0
14-15	0.004027802621179772	0.0	0.0	2.695773407876225E-4	0.0
16-17	0.004122947564987168	0.0	0.0	2.854348314221886E-4	0.0
18-19	0.004202235018159998	0.0	0.0	2.854348314221886E-4	0.0
20-21	0.004297379961967394	0.0	0.0	3.330073033258866E-4	0.0
22-23	0.0045035273402167526	0.0	0.0	3.8057977522958477E-4	0.0
24-25	0.004646244755927847	0.0	0.0	4.2815224713328286E-4	0.0
26-27	0.004836534643542639	0.0	0.0	5.07439700306113E-4	0.0
28-29	0.005153684456233961	0.0	0.0	5.39154681575245E-4	0.0
30-31	0.005264686890675923	0.0	0.0	5.39154681575245E-4	0.0
32-33	0.005724554119078337	0.0	0.0	5.39154681575245E-4	0.0
34-35	0.006358853744460978	0.0	0.0	5.550121722098111E-4	0.0
36-37	0.007199300748092979	0.0	0.0	6.025846441135091E-4	0.0
38-39	0.009102199624240902	0.0	0.0	6.342996253826412E-4	0.0
40-41	0.012241982769884977	0.0	0.0	6.660146066517733E-4	0.0
42-43	0.016507647750583238	0.0	0.0	7.135870785554714E-4	0.0
44-45	0.020678167787474104	0.0	0.0	7.611595504591695E-4	0.0
46-47	0.024943832768172365	0.0	0.0	7.928745317283015E-4	0.0
48-49	0.02933635767394716	0.0	0.0	8.245895129974336E-4	0.0
50-51	0.03620265111871425	0.0	0.0	8.245895129974336E-4	0.0
52-53	0.0439728215296516	0.0	0.0	8.245895129974336E-4	0.0
54-55	0.0534080284572184	0.0	0.0	8.245895129974336E-4	0.0
56-57	0.06363610991651349	0.0	0.0	8.245895129974336E-4	0.0
58-59	0.07535479549545779	0.0	0.0	8.245895129974336E-4	0.0
60-61	0.09056212901400659	0.0	0.0	8.245895129974336E-4	0.0
62-63	0.10976555017246606	0.0	0.0	8.404470036319997E-4	0.0
64-65	0.13229904436418438	0.0	0.0	8.563044942665657E-4	0.0
66-67	0.15849561889248748	0.0	0.0	8.563044942665657E-4	0.0
68-69	0.19151091439365397	0.0	0.0	8.721619849011318E-4	0.0
70-71	0.23367598199096504	0.0	0.0	8.880194755356977E-4	0.0
72-73	0.2805982967786459	0.0	0.0	9.514494380739619E-4	0.0
74-75	0.33486262973013087	0.0	0.0	9.514494380739619E-4	0.0
76-77	0.3960725435795558	0.0	0.0	9.514494380739619E-4	0.0
78-79	0.4704758896369396	0.0	0.0	9.514494380739619E-4	0.0
80-81	0.5589448298871835	0.0	0.0	9.67306928708528E-4	0.0
82-83	0.6669184836179436	0.0	0.0	0.001014879400612226	0.0
84-85	0.7943492783573163	0.0	0.0	0.001046594381881358	0.0
86-87	0.9425692433186049	0.0	0.0	0.001046594381881358	0.0
88	1.068414288994521	0.0	0.0	0.00107830936315049	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	5130	0.0	87.5392	1
AACGTGT	2325	0.0	73.2921	1
CGTGTGG	2670	0.0	71.83588	3
GTGGAAT	88875	0.0	71.446884	6
TGGAATC	89495	0.0	70.94892	7
AACGAGT	75890	0.0	70.66217	1
ACGAGTG	82540	0.0	70.576805	2
GGAATCA	90320	0.0	70.34658	8
GAATCAC	90495	0.0	70.205345	9
CGAGTGG	85585	0.0	69.98394	3
ACGTGTG	2580	0.0	69.97318	2
AGTGGAA	88090	0.0	69.7836	5
ATCGAGT	1685	0.0	69.56183	1
GAGTGGA	88805	0.0	69.227036	4
TCGAGTG	1890	0.0	63.181793	2
ATGAGTG	2855	0.0	58.457783	2
TGAGTGG	3315	0.0	54.171688	3
GCGAGTG	1095	0.0	50.66266	2
AATGAGT	3105	0.0	49.726032	1
GTGTGGA	4290	0.0	47.665462	4
>>END_MODULE