FastQCFastQC Report
Thu 26 May 2016
SRR2132755_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132755_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3557341
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2812657.906607772490745No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA783892.203584081481084No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG278900.784012553196334No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT221850.6236399602961875No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT95130.26741883895864915No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT87630.24633567600069825No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC84460.23742452579047102No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT79290.22289119879145688No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66230.1861783843606784No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT64880.18238341502824723No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA54970.1545255290398081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT59100.086.755411
AACGTGT27950.075.8030851
ACGTGTG31050.070.091732
CGTGTGG32800.069.9328463
ATCGAGT20950.069.4433441
TCGAGTG22350.068.352322
GTGGAAT1037450.067.4968956
TGGAATC1043850.067.06847
AACGAGT883700.066.342291
GGAATCA1055100.066.339928
GAATCAC1057700.066.2123959
ACGAGTG967750.065.994512
CGAGTGG1003250.065.672133
AGTGGAA1037750.065.146635
GAGTGGA1041200.064.984944
AGCGAGT11200.055.910041
ATGAGTG34350.055.421172
AATGAGT34100.053.2953221
TGAGTGG38550.053.283443
GTGTGGA47600.051.2502024