##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132755_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3557341
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.880759814704298	31.0	25.0	33.0	16.0	34.0
2	28.121415686604124	31.0	25.0	34.0	16.0	34.0
3	29.173052850429578	31.0	28.0	34.0	16.0	34.0
4	34.70482925308538	35.0	35.0	37.0	32.0	37.0
5	33.74514841281733	35.0	35.0	37.0	28.0	37.0
6	35.19006274630405	37.0	35.0	37.0	32.0	37.0
7	35.57992528689265	37.0	35.0	37.0	33.0	37.0
8	35.86696046288506	37.0	35.0	37.0	35.0	37.0
9	37.82589524029324	39.0	38.0	39.0	35.0	39.0
10-11	37.82199612013579	39.0	38.0	39.0	35.0	39.0
12-13	37.80673247237192	39.0	38.0	39.0	35.0	39.0
14-15	39.21438653196306	41.0	39.0	41.0	36.0	41.0
16-17	39.19526072985413	40.5	39.0	41.0	36.0	41.0
18-19	39.07711743124992	40.0	39.0	41.0	35.5	41.0
20-21	38.99916932337946	40.0	39.0	41.0	35.0	41.0
22-23	38.86291783666508	40.0	39.0	41.0	35.0	41.0
24-25	38.732150080636075	40.0	39.0	41.0	35.0	41.0
26-27	38.53139606239604	40.0	38.0	41.0	34.5	41.0
28-29	38.315469897319375	40.0	38.0	41.0	34.0	41.0
30-31	38.14072716110151	40.0	38.0	41.0	34.0	41.0
32-33	37.967123196792215	40.0	38.0	41.0	33.5	41.0
34-35	37.861923273591145	40.0	38.0	41.0	33.0	41.0
36-37	37.64938390781204	40.0	37.5	41.0	33.0	41.0
38-39	37.099963427739986	40.0	36.0	41.0	33.0	41.0
40-41	36.810319561717584	39.5	35.0	41.0	32.0	41.0
42-43	36.61351146825677	39.0	35.0	41.0	31.5	41.0
44-45	36.437439087228356	39.0	35.0	41.0	31.0	41.0
46-47	36.26265123304176	39.0	35.0	41.0	31.0	41.0
48-49	36.05471853836897	39.0	35.0	41.0	31.0	41.0
50-51	35.70758510359282	39.0	35.0	41.0	29.5	41.0
52-53	35.148636158299134	38.5	35.0	41.0	27.0	41.0
54-55	34.13157060287445	38.0	34.5	40.5	19.0	41.0
56-57	33.31308960822142	38.0	34.0	40.0	4.5	41.0
58-59	32.90410857435371	37.0	34.0	40.0	2.0	41.0
60-61	32.612069661019284	37.0	33.5	40.0	2.0	41.0
62-63	32.31585543809267	36.0	33.0	40.0	2.0	41.0
64-65	32.00125922704627	36.0	33.0	39.5	2.0	41.0
66-67	31.651730182740422	35.0	33.0	39.0	2.0	41.0
68-69	31.317841190934466	35.0	33.0	39.0	2.0	41.0
70-71	30.978355041026433	35.0	32.5	38.0	2.0	40.0
72-73	30.65211333408858	35.0	32.0	37.0	2.0	40.0
74-75	30.316079903500956	35.0	32.0	37.0	2.0	39.0
76-77	29.98684298187888	35.0	32.0	36.0	2.0	39.0
78-79	29.65832850997416	35.0	31.0	36.0	2.0	38.5
80-81	29.352248631773	35.0	31.0	35.0	2.0	37.0
82-83	29.053981752100796	35.0	31.0	35.0	2.0	37.0
84-85	28.782040293578827	35.0	31.0	35.0	2.0	36.5
86-87	28.583705919674273	35.0	30.5	35.0	2.0	36.0
88-89	28.354377750122914	35.0	30.0	35.0	2.0	36.0
90-91	28.15407758210416	34.5	30.0	35.0	2.0	36.0
92-93	27.979939370445507	34.0	30.0	35.0	2.0	35.0
94-95	27.775452086263307	34.0	29.5	35.0	2.0	35.0
96-97	27.556856371092906	34.0	29.0	35.0	2.0	35.0
98-99	27.37013361946465	34.0	29.0	35.0	2.0	35.0
100	27.261277454143418	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.2767340851143629
1101	2	2.3608347432857464
1101	3	1.132657916720472
1101	4	0.7000605800699162
1101	5	2.29036837978677
1101	6	1.08852789009687
1101	7	0.6007888639633094
1101	8	0.4184926494809105
1101	9	0.322175141829959
1101	10-11	0.49196401832061554
1101	12-13	0.40809549694004943
1101	14-15	0.5556713221518805
1101	16-17	0.5299796785958151
1101	18-19	0.47803877641910475
1101	20-21	0.4560723210450277
1101	22-23	0.4964442995699869
1101	24-25	0.5251439291561297
1101	26-27	0.47623630300464725
1101	28-29	0.46974848644180867
1101	30-31	0.481437187190771
1101	32-33	0.5596086119433181
1101	34-35	0.6052911704205144
1101	36-37	0.6405599392314585
1101	38-39	0.6766625117535412
1101	40-41	0.6312818096178745
1101	42-43	0.6681822157064019
1101	44-45	0.7307783346654091
1101	46-47	0.6906710479498486
1101	48-49	0.6569964797081553
1101	50-51	0.6909883355800943
1101	52-53	0.7928699527330565
1101	54-55	0.7275560565043833
1101	56-57	0.7902356950174152
1101	58-59	0.7550698123827146
1101	60-61	0.686985962766542
1101	62-63	0.6943027401543702
1101	64-65	0.7474395410070045
1101	66-67	0.6839500879593672
1101	68-69	0.7974988527351599
1101	70-71	0.7855899047786785
1101	72-73	0.827203952343698
1101	74-75	0.8094903180629025
1101	76-77	0.7308220908947725
1101	78-79	0.7521597212091748
1101	80-81	0.7780738514276031
1101	82-83	0.7944883035018506
1101	84-85	0.79274476849724
1101	86-87	0.8177476873308684
1101	88-89	0.7929207425969871
1101	90-91	0.7722523336031806
1101	92-93	0.8170457552258235
1101	94-95	0.8565572332974014
1101	96-97	0.8671911286386873
1101	98-99	0.8323530208251597
1101	100	0.8316193893766162
1102	1	1.6298796829218318
1102	2	3.1826511644345423
1102	3	3.298399609080871
1102	4	1.454774934300815
1102	5	2.6801646113729305
1102	6	1.2442202048165214
1102	7	0.7668395205808665
1102	8	0.5388007001832094
1102	9	0.4289132484874898
1102	10-11	0.5454413735792656
1102	12-13	0.5127717102551088
1102	14-15	0.6788332725915254
1102	16-17	0.5606399503783877
1102	18-19	0.48849313293388974
1102	20-21	0.44713323231600555
1102	22-23	0.3944823491303353
1102	24-25	0.44038197871648777
1102	26-27	0.5112165017028261
1102	28-29	0.5728072508588511
1102	30-31	0.6148406358604035
1102	32-33	0.5987973752184104
1102	34-35	0.6109981020180939
1102	36-37	0.6219316875470966
1102	38-39	0.6735825985412589
1102	40-41	0.7092247124384699
1102	42-43	0.7529593768346459
1102	44-45	0.7990413014348547
1102	46-47	0.7990572532713003
1102	48-49	0.7656951207952858
1102	50-51	0.7702796453893939
1102	52-53	0.7936668009687509
1102	54-55	0.8285100361777893
1102	56-57	0.7034110290359195
1102	58-59	0.7365581302998123
1102	60-61	0.712538686302004
1102	62-63	0.7578270064556207
1102	64-65	0.7218631107226656
1102	66-67	0.8389931392326346
1102	68-69	0.7742397000573007
1102	70-71	0.745325887421135
1102	72-73	0.7372725603144623
1102	74-75	0.8206875773982922
1102	76-77	0.8373266472496894
1102	78-79	0.8160375789230052
1102	80-81	0.775809103226166
1102	82-83	0.7941933623119439
1102	84-85	0.7326270112744453
1102	86-87	0.7840445013082586
1102	88-89	0.8185846000824277
1102	90-91	0.8160510317874881
1102	92-93	0.8340331710074835
1102	94-95	0.8428945177555462
1102	96-97	0.8617466156776246
1102	98-99	0.907236474062568
1102	100	0.932762429230447
1103	1	1.376177366391179
1103	2	3.0691388753910793
1103	3	3.2890105281271396
1103	4	1.429361466746883
1103	5	2.640577452494277
1103	6	1.2451028838996692
1103	7	0.7229758936908226
1103	8	0.49935420202367453
1103	9	0.4185322228512618
1103	10-11	0.4695276359626206
1103	12-13	0.4612333876022987
1103	14-15	0.5730986394296878
1103	16-17	0.41966386905021125
1103	18-19	0.4319417034065012
1103	20-21	0.42296341423191564
1103	22-23	0.40306864981692314
1103	24-25	0.3909767457379445
1103	26-27	0.48850177980802556
1103	28-29	0.5392329629210337
1103	30-31	0.5965609596675563
1103	32-33	0.5792189317190761
1103	34-35	0.5996323484614337
1103	36-37	0.5381958029310923
1103	38-39	0.5631476632513639
1103	40-41	0.6356351837574152
1103	42-43	0.6638988299351851
1103	44-45	0.7729584387784456
1103	46-47	0.7534327511323511
1103	48-49	0.7661964873354847
1103	50-51	0.7529850939653926
1103	52-53	0.7795711711988176
1103	54-55	0.8049943418808851
1103	56-57	0.7795538804759161
1103	58-59	0.7924432807197661
1103	60-61	0.824112076833245
1103	62-63	0.725215693265369
1103	64-65	0.7553336889390465
1103	66-67	0.7855268093521133
1103	68-69	0.7528749830099315
1103	70-71	0.7302897121886289
1103	72-73	0.8111209517525779
1103	74-75	0.870556344033961
1103	76-77	0.760639790111064
1103	78-79	0.7034127311437715
1103	80-81	0.7393221095364098
1103	82-83	0.7612112751091189
1103	84-85	0.78714881098146
1103	86-87	0.8479101265505911
1103	88-89	0.8433793662455216
1103	90-91	0.8982086061276355
1103	92-93	0.9198349015370546
1103	94-95	0.9061034911392483
1103	96-97	0.9112678900749103
1103	98-99	0.9189095793744428
1103	100	0.9889931170428454
1104	1	6.065474825855102
1104	2	5.119656142462702
1104	3	4.154057093675203
1104	4	1.8916918557900786
1104	5	2.890263029992532
1104	6	1.3077147213725908
1104	7	0.7228234318645406
1104	8	0.5693667235549853
1104	9	0.47005333113037295
1104	10-11	0.5294487919831639
1104	12-13	0.399414015458575
1104	14-15	0.5398721451971511
1104	16-17	0.43388091316371913
1104	18-19	0.3877144965837189
1104	20-21	0.3954533910038691
1104	22-23	0.3560360691173088
1104	24-25	0.3356394024071605
1104	26-27	0.36125029477418735
1104	28-29	0.3889023959068325
1104	30-31	0.44899274274632717
1104	32-33	0.4809419452766548
1104	34-35	0.49983602638759095
1104	36-37	0.44005623552775575
1104	38-39	0.4830855570210346
1104	40-41	0.483479340482063
1104	42-43	0.5473855078874763
1104	44-45	0.6058861535954492
1104	46-47	0.48578215906096034
1104	48-49	0.5981322821772892
1104	50-51	0.6703957429875089
1104	52-53	0.6417259198112504
1104	54-55	0.6571692252286638
1104	56-57	0.5413204686799702
1104	58-59	0.554727425551441
1104	60-61	0.6038781438365035
1104	62-63	0.6089035631996467
1104	64-65	0.606093038949389
1104	66-67	0.6404439151198602
1104	68-69	0.6114511161096452
1104	70-71	0.6167503986058414
1104	72-73	0.5806986025494574
1104	74-75	0.6242458736184595
1104	76-77	0.6326722966560894
1104	78-79	0.6233597212091766
1104	80-81	0.6269726168597067
1104	82-83	0.6032652582343587
1104	84-85	0.5853011470980611
1104	86-87	0.6018991276600865
1104	88-89	0.5522688907451361
1104	90-91	0.4968811401875399
1104	92-93	0.5451576893822043
1104	94-95	0.6214724596348518
1104	96-97	0.5155763138238711
1104	98-99	0.5666970537469709
1104	100	0.5818679490473961
1105	1	1.6967763032413536
1105	2	3.0840375727193035
1105	3	3.2176191050825658
1105	4	1.3815791817340681
1105	5	2.598139955148355
1105	6	1.1893795644886396
1105	7	0.6727584252149583
1105	8	0.4506173828254134
1105	9	0.3047904468317242
1105	10-11	0.38436314715291076
1105	12-13	0.3733103812497234
1105	14-15	0.44256951094211416
1105	16-17	0.3656058955314663
1105	18-19	0.3519284359563457
1105	20-21	0.25731487423651345
1105	22-23	0.2704819682656492
1105	24-25	0.28721702533825777
1105	26-27	0.3022731377620005
1105	28-29	0.35727695389402214
1105	30-31	0.4126985561154015
1105	32-33	0.4677940142999404
1105	34-35	0.4760798154569059
1105	36-37	0.4599081866579766
1105	38-39	0.44226735157410246
1105	40-41	0.43131323122209153
1105	42-43	0.5054793769655035
1105	44-45	0.5420470452255302
1105	46-47	0.608700570855234
1105	48-49	0.5806906030491206
1105	50-51	0.5779917792985714
1105	52-53	0.5445496310711349
1105	54-55	0.6501247354195243
1105	56-57	0.643142736956321
1105	58-59	0.6148276277621818
1105	60-61	0.6004809758899086
1105	62-63	0.6368446883682992
1105	64-65	0.5774985793718912
1105	66-67	0.5713653631776268
1105	68-69	0.5831612342991654
1105	70-71	0.5739505830981422
1105	72-73	0.5716789695622886
1105	74-75	0.5585121754371158
1105	76-77	0.5544547708523311
1105	78-79	0.6104939890001688
1105	80-81	0.5894123000789726
1105	82-83	0.5248920860077604
1105	84-85	0.4753125985823097
1105	86-87	0.4961556395283324
1105	88-89	0.4816580185226016
1105	90-91	0.45035224983705646
1105	92-93	0.5192684688759819
1105	94-95	0.5701939265830802
1105	96-97	0.5641496215930246
1105	98-99	0.522079630530861
1105	100	0.5332442437166307
1106	1	-0.7739153687088738
1106	2	1.84253148829292
1106	3	2.6931345800769755
1106	4	1.1348506495833206
1106	5	2.413584798191266
1106	6	1.0984339256259474
1106	7	0.5974952967118412
1106	8	0.44514369218200756
1106	9	0.30171286664513985
1106	10-11	0.40373283659270953
1106	12-13	0.35295883137677464
1106	14-15	0.41707624325967885
1106	16-17	0.34548536104092165
1106	18-19	0.27460604994883653
1106	20-21	0.268720848022852
1106	22-23	0.2637368958405375
1106	24-25	0.26999733310626084
1106	26-27	0.26045172836048636
1106	28-29	0.256002530356632
1106	30-31	0.2796746361591005
1106	32-33	0.22951195520714407
1106	34-35	0.33890370490137656
1106	36-37	0.3309385776514162
1106	38-39	0.3806692203489348
1106	40-41	0.3231238045422913
1106	42-43	0.37382520367948047
1106	44-45	0.521008854360872
1106	46-47	0.4567520365849589
1106	48-49	0.45707548224594774
1106	50-51	0.44958769561540635
1106	52-53	0.4773896481981339
1106	54-55	0.4469355114310076
1106	56-57	0.4526419181223531
1106	58-59	0.4995185379702676
1106	60-61	0.4950282004361952
1106	62-63	0.4270940895971691
1106	64-65	0.4484099481569572
1106	66-67	0.49062646443514524
1106	68-69	0.46376017900681177
1106	70-71	0.44129684508542155
1106	72-73	0.44535877966356807
1106	74-75	0.4701203081324188
1106	76-77	0.36813794657170007
1106	78-79	0.44913705763199374
1106	80-81	0.428985072877456
1106	82-83	0.45284147574062317
1106	84-85	0.43431369710807743
1106	86-87	0.5249253106344085
1106	88-89	0.5779472900978178
1106	90-91	0.5137073484989081
1106	92-93	0.5021035947932937
1106	94-95	0.46368516003247606
1106	96-97	0.5300181836977131
1106	98-99	0.5347241562107925
1106	100	0.5531339430564124
1107	1	-0.8522534652977001
1107	2	1.743091847733698
1107	3	-0.6219273892588362
1107	4	-0.044920237043882594
1107	5	1.8793254900795944
1107	6	0.8044254410934215
1107	7	0.3938444019842322
1107	8	0.3071460599666196
1107	9	0.2850944942175886
1107	10-11	0.28475461975845207
1107	12-13	0.29045070180299604
1107	14-15	0.3338693699629758
1107	16-17	0.2965822854109348
1107	18-19	0.3013683456516745
1107	20-21	0.26893249989826273
1107	22-23	0.2927953662600302
1107	24-25	0.2084565248659942
1107	26-27	0.23254463195431896
1107	28-29	0.2576055908897601
1107	30-31	0.21075321968828575
1107	32-33	0.24251304820493402
1107	34-35	0.2713632494432545
1107	36-37	0.2966736103986847
1107	38-39	0.3611379574254201
1107	40-41	0.3965370553949441
1107	42-43	0.3503637108259312
1107	44-45	0.3994676560751884
1107	46-47	0.46393400896451453
1107	48-49	0.3967409416872343
1107	50-51	0.41511294835699175
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2215	28-29	-0.6154066774608467
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2216	1	3.0270667454653513
2216	2	-1.62437560338644
2216	3	-2.1115622002749213
2216	4	-0.7680907063617255
2216	5	-2.4444976138975534
2216	6	-1.0341426971233645
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2216	12-13	-0.2601871418409871
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2216	56-57	-0.13449182996058084
2216	58-59	-0.1313463391936125
2216	60-61	-0.15746549449907832
2216	62-63	-0.08910434514181986
2216	64-65	-0.11096480749431592
2216	66-67	-0.11364588104893159
2216	68-69	-0.17563512597098807
2216	70-71	-0.12250238008266834
2216	72-73	-0.18094682331160072
2216	74-75	-0.2375197559651525
2216	76-77	-0.31733536626731507
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2216	80-81	-0.28400485644629114
2216	82-83	-0.31351449886566485
2216	84-85	-0.23561784129379149
2216	86-87	-0.2946399919153926
2216	88-89	-0.26657035850566757
2216	90-91	-0.2633944438467388
2216	92-93	-0.30249250295781493
2216	94-95	-0.37114960868979807
2216	96-97	-0.3644296682414385
2216	98-99	-0.43414248054669
2216	100	-0.47203485645175647
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1958.0
3	445.0
4	546.0
5	1332.0
6	3306.0
7	5474.0
8	6665.0
9	9150.0
10	7796.0
11	10198.0
12	9301.0
13	12914.0
14	13420.0
15	15061.0
16	17524.0
17	20234.0
18	27558.0
19	46199.0
20	89507.0
21	102261.0
22	45774.0
23	29803.0
24	29909.0
25	31506.0
26	32553.0
27	35707.0
28	41335.0
29	47840.0
30	55658.0
31	67613.0
32	85673.0
33	117241.0
34	170732.0
35	319806.0
36	476188.0
37	736199.0
38	672978.0
39	159107.0
40	870.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.80983989023876	42.741604498093984	23.232833338681775	20.21572227298548
2	15.097650495786546	41.79953423727136	23.534258636626358	19.568556630315733
3	19.031366374331395	23.612784938338034	17.15906001798597	40.1967886693446
4	35.69950139725149	25.576097427826006	19.43620248944366	19.288198685478843
5	17.497308314006606	40.53381123058543	20.21561599550214	21.75326445990583
6	40.129748726058686	21.630166930240733	21.87805306624495	16.362031277455632
7	19.691614847466013	21.364400490267734	40.455521696596236	18.488462965670013
8	39.0745784562121	21.735813350477223	21.142505033956542	18.047103159354137
9	38.202550725387304	23.736970956678036	21.418300916330484	16.642177401604176
10-11	16.8895531803108	44.189550565998594	21.326251826856073	17.594644426834538
12-13	16.77401744730123	23.287983918325512	31.417370446071942	28.520628188301316
14-15	17.181034935925457	33.2226092466255	21.198473803888916	28.397882013560128
16-17	27.928430251696422	22.58421388334714	31.667107539029853	17.82024832592658
18-19	17.498884700679522	22.940955056037645	31.878360831868523	27.68179941141431
20-21	28.010752975326238	33.64163008269379	21.36175587327726	16.98586106870272
22-23	27.30117804281344	23.274125252541154	32.07560084906114	17.34909585558427
24-25	17.049279222880234	33.60557787403569	21.34532506161203	27.99981784147204
26-27	17.66147805341124	27.681630746110645	37.397426898349075	17.259464302129036
28-29	27.446314536615972	22.931622242568256	32.517250946704294	17.104812274111477
30-31	24.24434430098211	24.630180238554583	33.036894691849895	18.08858076861341
32-33	19.549250409224193	26.384425895633846	34.117884678471924	19.948439016670036
34-35	19.53593990567674	26.149011298045366	34.51559746451071	19.79945133176718
36-37	20.022595528514135	26.006404783797787	34.709309565768365	19.261690121919713
38-39	20.685112841304782	25.71334881868227	34.06367283878605	19.5378655012269
40-41	19.15010396810427	27.49609891208068	33.90203525610843	19.45176186370663
42-43	19.21512592592801	26.287240398958822	34.62525540721155	19.872378267901627
44-45	19.4670682400141	26.98010958184779	33.474820097370475	20.07800208076763
46-47	20.255873698922873	26.131343607486603	34.055309850812726	19.55747284277779
48-49	19.469218722635812	26.504150150351062	33.89535892117174	20.131272205841384
50-51	20.05978622797196	27.316653084424576	32.93998241945318	19.683578268150285
52-53	20.24028672037637	26.889396228945078	32.831093851616664	20.03922319906188
54-55	20.566161635896023	27.95443281934456	30.42660234146797	21.052803203291447
56-57	21.127170546765125	28.757335886551218	28.988576017874024	21.126917548809633
58-59	22.275542322200767	29.095130323463508	27.60865489139219	21.02067246294353
60-61	21.62311681674599	29.64291306343699	27.301655927840486	21.432314191976534
62-63	20.595239868902006	29.67420310518621	27.100917286769686	22.629639739142092
64-65	21.073858255365455	29.552859846722594	28.2185486294398	21.154733268472153
66-67	21.365275739738117	28.803516110352117	28.63669202269111	21.19451612721865
68-69	21.6069671139202	28.972370093280347	28.35866733045834	21.061995462341113
70-71	19.80864359081685	30.203134869555658	28.67557538060029	21.312646159027206
72-73	19.993135298019286	29.441630144643653	28.7584629870421	21.806771570294956
74-75	20.30167309588448	29.90537137781101	27.82789889320002	21.96505663310449
76-77	21.25527503258067	29.191465269961352	27.960778945072196	21.592480752385782
78-79	20.964693027365904	29.517670352906684	27.321780937577174	22.195855682150242
80-81	21.46938379659335	30.221789935224884	26.925631253412867	21.383195014768894
82-83	21.107927049187012	30.34003869787797	26.95537993551853	21.59665431741649
84-85	21.070419328155715	30.573180904886364	26.22426153561798	22.132138231339944
86-87	20.913806640300518	30.728235346701567	26.55877047422244	21.79918753877547
88-89	22.372426483713536	29.899228103237785	26.297788151318642	21.43055726173004
90-91	21.427100625931438	30.736138829633447	26.113132050829574	21.723628493605542
92-93	20.753562843708266	30.858807182105963	26.169588465092325	22.21804150909345
94-95	20.98341738489995	31.138575452444805	25.85323584476041	22.02477131789483
96-97	20.981849697073283	30.192342723236976	26.284810147932586	22.540997431757155
98-99	21.916900000323277	30.264554756373236	25.29756629751668	22.520978945786805
100	20.90763859860497	31.31063341973682	25.175039446597896	22.606688535060314
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	79705.0
1	43482.0
2	5285.5
3	2841.0
4	2399.0
5	2264.5
6	2256.0
7	2622.5
8	2550.5
9	2241.5
10	2274.0
11	3558.0
12	23779.0
13	26786.0
14	9790.5
15	9227.5
16	10307.0
17	11792.5
18	13434.0
19	15230.0
20	17039.0
21	19213.5
22	22058.0
23	24928.0
24	27890.0
25	31121.5
26	34814.5
27	38805.0
28	42300.5
29	45261.0
30	48329.0
31	52149.0
32	56753.5
33	61436.5
34	65564.0
35	69726.5
36	74802.5
37	79133.0
38	81786.0
39	82783.0
40	83279.5
41	84518.0
42	85712.5
43	87279.0
44	90240.0
45	94452.5
46	100352.5
47	107570.5
48	112902.5
49	124591.0
50	160460.0
51	166102.0
52	131109.0
53	115655.0
54	113986.5
55	114401.0
56	104582.0
57	88605.5
58	76639.5
59	62868.0
60	51511.5
61	43753.0
62	36200.5
63	27768.0
64	20897.5
65	15826.0
66	11994.0
67	9186.0
68	7221.0
69	5550.0
70	4132.5
71	3085.0
72	2253.0
73	1527.5
74	1074.0
75	810.5
76	550.5
77	329.5
78	199.0
79	130.5
80	68.5
81	34.5
82	24.5
83	18.0
84	12.0
85	5.5
86	2.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.137827663977111
2	0.004638295850749197
3	0.003485749609047882
4	0.0
5	0.0025580904388980423
6	0.013099671917873491
7	0.0028391992783373876
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	8.854928442339376E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	2.1083162957950897E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	5.622176788786906E-5
64-65	0.0
66-67	2.811088394393453E-5
68-69	0.0
70-71	0.0
72-73	3.513860492991816E-4
74-75	3.7949693324311614E-4
76-77	4.7788502704688697E-4
78-79	3.513860492991816E-4
80-81	9.417146121218068E-4
82-83	0.001124435357757381
84-85	0.001222823451561152
86-87	5.622176788786906E-5
88-89	0.0
90-91	7.027720985983631E-5
92-93	0.0
94-95	2.811088394393453E-5
96-97	0.0012649897774770539
98-99	6.887166566263959E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3557341.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.98991771638039
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.98942410759781	47.86550368938175
2	10.472622208101221	11.93667755828853
3	2.707623663520166	4.62921749372816
4	1.0010689981470482	2.2820335933127853
5	0.4973179972595383	1.4171055871348088
6	0.2839180339647145	0.9708279236307342
7	0.18953622925975902	0.7561157876850675
8	0.12543007081887095	0.5718599532101777
9	0.0919775831791814	0.4717615407419772
>10	0.5355264689189483	6.371649242221691
>50	0.06425522913884359	2.4238577234663596
>100	0.035597833156678874	3.7816227800627473
>500	0.0027764200745423926	1.1439598348611015
>1k	0.002379788634039726	2.2960853613352397
>5k	3.4705250913079333E-4	1.5048325838247854
>10k+	1.983157195033105E-4	11.576889347114017
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	281265	7.906607772490745	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	78389	2.203584081481084	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	27890	0.784012553196334	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	22185	0.6236399602961875	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9513	0.26741883895864915	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8763	0.24633567600069825	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	8446	0.23742452579047102	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7929	0.22289119879145688	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6623	0.1861783843606784	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6488	0.18238341502824723	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA	5497	0.1545255290398081	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.005622176788786906	2.811088394393453E-5	0.0	0.0	2.811088394393453E-5
2	0.005622176788786906	8.433265183180359E-5	0.0	0.0	2.811088394393453E-5
3	0.005622176788786906	8.433265183180359E-5	0.0	2.811088394393453E-5	2.811088394393453E-5
4	0.005622176788786906	1.1244353577573811E-4	0.0	2.811088394393453E-5	2.811088394393453E-5
5	0.005622176788786906	1.6866530366360717E-4	0.0	5.622176788786906E-5	2.811088394393453E-5
6	0.005622176788786906	1.967761876075417E-4	0.0	5.622176788786906E-5	2.811088394393453E-5
7	0.005622176788786906	1.967761876075417E-4	0.0	5.622176788786906E-5	2.811088394393453E-5
8	0.005622176788786906	2.2488707155147623E-4	0.0	5.622176788786906E-5	2.811088394393453E-5
9	0.005622176788786906	2.2488707155147623E-4	0.0	5.622176788786906E-5	2.811088394393453E-5
10-11	0.00565028767273084	2.67053397467378E-4	0.0	5.622176788786906E-5	5.622176788786906E-5
12-13	0.005678398556674775	3.232751653552471E-4	0.0	5.622176788786906E-5	5.622176788786906E-5
14-15	0.005734620324562643	3.6544149127114886E-4	0.0	5.622176788786906E-5	5.622176788786906E-5
16-17	0.0058189529763944476	3.7949693324311614E-4	0.0	5.622176788786906E-5	8.433265183180359E-5
18-19	0.005945451954142153	4.919404690188543E-4	0.0	5.622176788786906E-5	8.433265183180359E-5
20-21	0.006071950931889858	5.622176788786906E-4	0.0	5.622176788786906E-5	8.433265183180359E-5
22-23	0.006142228141749695	5.903285628226251E-4	0.0	5.622176788786906E-5	8.433265183180359E-5
24-25	0.006282782561469367	6.324948887385269E-4	0.0	5.622176788786906E-5	9.838809380377086E-5
26-27	0.006353059771329203	6.465503307104941E-4	0.0	5.622176788786906E-5	1.264989777477054E-4
28-29	0.006451447865132975	6.887166566263959E-4	0.0	5.622176788786906E-5	1.546098616916399E-4
30-31	0.006704445820628385	7.308829825422977E-4	0.0	5.622176788786906E-5	1.6866530366360717E-4
32-33	0.007168275405703305	7.44938424514265E-4	0.0	5.622176788786906E-5	1.6866530366360717E-4
34-35	0.007828881178385766	7.589938664862323E-4	0.0	5.622176788786906E-5	1.6866530366360717E-4
36-37	0.009431201563190035	8.011601924021341E-4	0.0	5.622176788786906E-5	1.6866530366360717E-4
38-39	0.012129846421807748	8.433265183180359E-4	0.0	5.622176788786906E-5	1.6866530366360717E-4
40-41	0.017189805531715965	8.573819602900031E-4	0.0	5.622176788786906E-5	1.6866530366360717E-4
42-43	0.02255898436500746	8.714374022619703E-4	0.0	5.622176788786906E-5	1.6866530366360717E-4
44-45	0.02850443631914961	8.995482862059049E-4	0.0	5.622176788786906E-5	1.6866530366360717E-4
46-47	0.034393666505403896	9.557700540937739E-4	0.0	5.622176788786906E-5	1.6866530366360717E-4
48-49	0.040521839205181626	9.838809380377084E-4	0.0	5.622176788786906E-5	1.8272074563557443E-4
50-51	0.04953137750921264	0.001011991821981643	0.0	7.027720985983631E-5	1.967761876075417E-4
52-53	0.059946460010440385	0.0010541581478975448	0.0	8.433265183180359E-5	1.967761876075417E-4
54-55	0.07471872952297798	0.001068213589869512	0.0	8.433265183180359E-5	1.967761876075417E-4
56-57	0.09067165616116082	0.0010822690318414794	0.0	8.433265183180359E-5	1.967761876075417E-4
58-59	0.10829718039400776	0.001110379915785414	0.0	1.1244353577573811E-4	2.1083162957950897E-4
60-61	0.1301815035443608	0.0011244353577573812	0.0	1.1244353577573811E-4	2.2488707155147623E-4
62-63	0.15712578580462205	0.0011244353577573812	0.0	1.1244353577573811E-4	2.2488707155147623E-4
64-65	0.18901758363901577	0.0011806571256452502	0.0	0.001068213589869512	2.2488707155147623E-4
66-67	0.2264893919362805	0.0011806571256452502	0.0	0.001068213589869512	2.2488707155147623E-4
68-69	0.27440439361871694	0.0012368788935331193	0.0	0.0010963244738134467	2.2488707155147623E-4
70-71	0.33936864641314957	0.001279045219449021	0.0	0.0010963244738134467	2.2488707155147623E-4
72-73	0.41016590762594873	0.0013493224293088574	0.0	0.0010963244738134467	2.2488707155147623E-4
74-75	0.4881173888024791	0.001377433313252792	0.0	0.0010963244738134467	2.3894251352344348E-4
76-77	0.5730684800810493	0.001377433313252792	0.0	0.0010963244738134467	2.5299795549541074E-4
78-79	0.6724685657068018	0.0014055441971967263	0.0	0.0010963244738134467	2.811088394393453E-4
80-81	0.7953552948677116	0.0015039322910004973	0.0	0.0010963244738134467	2.811088394393453E-4
82-83	0.9460296328072006	0.0015460986169163991	0.0	0.0010963244738134467	2.811088394393453E-4
84-85	1.124126138033998	0.0015742095008603337	0.0	0.0010963244738134467	2.9516428141131254E-4
86-87	1.3243037425987556	0.0016163758267762353	0.0	0.0010963244738134467	3.232751653552471E-4
88	1.4895676293051467	0.0016585421526921372	0.0	0.0010963244738134467	3.3733060732721434E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	5910	0.0	86.75541	1
AACGTGT	2795	0.0	75.803085	1
ACGTGTG	3105	0.0	70.09173	2
CGTGTGG	3280	0.0	69.932846	3
ATCGAGT	2095	0.0	69.443344	1
TCGAGTG	2235	0.0	68.35232	2
GTGGAAT	103745	0.0	67.496895	6
TGGAATC	104385	0.0	67.0684	7
AACGAGT	88370	0.0	66.34229	1
GGAATCA	105510	0.0	66.33992	8
GAATCAC	105770	0.0	66.212395	9
ACGAGTG	96775	0.0	65.99451	2
CGAGTGG	100325	0.0	65.67213	3
AGTGGAA	103775	0.0	65.14663	5
GAGTGGA	104120	0.0	64.98494	4
AGCGAGT	1120	0.0	55.91004	1
ATGAGTG	3435	0.0	55.42117	2
AATGAGT	3410	0.0	53.295322	1
TGAGTGG	3855	0.0	53.28344	3
GTGTGGA	4760	0.0	51.250202	4
>>END_MODULE