FastQCFastQC Report
Thu 26 May 2016
SRR2132754_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132754_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3309639
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2544097.686910868526748No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800862.419780525912343No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT200830.6068033401830231No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG101850.3077374904030319No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT85730.2590312719906914No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70760.2137997527827053No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68030.20555111901932507No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT58920.1780254583657009No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56480.17065305309733175No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC50070.15128538188001775No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA34260.10351582151406846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT48400.085.880571
AACGAGT608400.079.261081
ACGAGTG669700.078.480752
CGAGTGG701450.077.2158663
GTGGAAT744000.077.143336
TGGAATC750050.076.486787
GAATCAC755850.075.95869
GAGTGGA733550.075.878884
GGAATCA756500.075.8684848
AGTGGAA737050.075.295345
AACGTGT23800.065.863811
CGTGTGG27600.064.210743
ATCGAGT17950.062.67771
TCGAGTG18800.062.514942
ACGTGTG27400.060.050852
ATGAGTG32050.049.8715132
AATGAGT30400.048.6223141
TGAGTGG35700.048.0618023
GCGAGTG12100.043.9030532
GTGTGGA43800.043.787324