##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132754_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3309639
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.92022422989335	31.0	25.0	33.0	16.0	34.0
2	28.190057586340988	31.0	25.0	34.0	16.0	34.0
3	29.256670591566028	31.0	28.0	34.0	16.0	34.0
4	34.773902531363696	35.0	35.0	37.0	32.0	37.0
5	33.842833614179675	35.0	35.0	37.0	28.0	37.0
6	35.27805751624271	37.0	35.0	37.0	32.0	37.0
7	35.66037957614108	37.0	35.0	37.0	33.0	37.0
8	35.938617474594665	37.0	35.0	37.0	35.0	37.0
9	37.909394952138285	39.0	38.0	39.0	35.0	39.0
10-11	37.90172991676736	39.0	38.0	39.0	35.0	39.0
12-13	37.877057588456026	39.0	38.0	39.0	35.0	39.0
14-15	39.29607141443523	41.0	39.0	41.0	36.0	41.0
16-17	39.26597024630179	40.5	39.0	41.0	36.0	41.0
18-19	39.11974221357677	40.0	39.0	41.0	35.5	41.0
20-21	39.03283258385582	40.0	39.0	41.0	35.0	41.0
22-23	38.8953290676113	40.0	39.0	41.0	35.0	41.0
24-25	38.77301225299799	40.0	39.0	41.0	35.0	41.0
26-27	38.57664264894147	40.0	38.5	41.0	35.0	41.0
28-29	38.40998081663892	40.0	38.0	41.0	35.0	41.0
30-31	38.283962994151324	40.0	38.0	41.0	34.0	41.0
32-33	38.121400702614395	40.0	38.0	41.0	34.0	41.0
34-35	38.02281517712355	40.0	38.0	41.0	34.0	41.0
36-37	37.85089416096439	40.0	38.0	41.0	33.5	41.0
38-39	37.342289294995616	40.0	36.0	41.0	33.0	41.0
40-41	37.07071662498538	40.0	35.0	41.0	33.0	41.0
42-43	36.90216682242384	40.0	35.0	41.0	33.0	41.0
44-45	36.75049257638069	39.5	35.0	41.0	33.0	41.0
46-47	36.60431258514902	39.0	35.0	41.0	32.0	41.0
48-49	36.41963670358005	39.0	35.0	41.0	31.5	41.0
50-51	36.11880177868342	39.0	35.0	41.0	31.0	41.0
52-53	35.64799453958574	39.0	35.0	41.0	29.5	41.0
54-55	34.74370543131744	38.5	35.0	40.5	24.5	41.0
56-57	33.95169201233126	38.0	34.5	40.0	16.0	41.0
58-59	33.56938082975213	37.5	34.0	40.0	9.0	41.0
60-61	33.29831939374657	37.0	34.0	40.0	7.0	41.0
62-63	32.99653648026265	36.5	34.0	40.0	3.5	41.0
64-65	32.70080301809351	36.0	34.0	39.5	2.0	41.0
66-67	32.39061239005221	35.5	33.5	39.0	2.0	41.0
68-69	32.0725147062867	35.0	33.0	39.0	2.0	41.0
70-71	31.741469840064127	35.0	33.0	38.5	2.0	40.0
72-73	31.41965377492832	35.0	33.0	37.0	2.0	40.0
74-75	31.095786881892558	35.0	33.0	37.0	2.0	39.0
76-77	30.780415930559194	35.0	33.0	36.0	2.0	39.0
78-79	30.466917086727584	35.0	33.0	36.0	2.0	38.5
80-81	30.180112997218124	35.0	32.0	35.5	2.0	37.0
82-83	29.917116186991997	35.0	32.0	35.0	2.0	37.0
84-85	29.669166486133385	35.0	32.0	35.0	2.0	36.5
86-87	29.474036896471187	35.0	32.0	35.0	2.0	36.0
88-89	29.27847916343746	35.0	32.0	35.0	2.0	36.0
90-91	29.096698461675125	35.0	31.5	35.0	2.0	36.0
92-93	28.926785217360568	35.0	31.0	35.0	2.0	35.0
94-95	28.747950758375765	35.0	31.0	35.0	2.0	35.0
96-97	28.573406344317313	35.0	31.0	35.0	2.0	35.0
98-99	28.40815644848275	35.0	31.0	35.0	2.0	35.0
100	28.2997843571459	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.16275411372431137
1101	2	2.338471258097691
1101	3	1.1886327263907503
1101	4	0.6278144172242435
1101	5	2.2412518252597593
1101	6	0.975075851172214
1101	7	0.536523202184874
1101	8	0.3827894391118605
1101	9	0.36268007193143603
1101	10-11	0.4693085750671102
1101	12-13	0.4188649112419611
1101	14-15	0.547649526401301
1101	16-17	0.5037870571217056
1101	18-19	0.4662143925396123
1101	20-21	0.47172271528989995
1101	22-23	0.46001186851134435
1101	24-25	0.39324565208200823
1101	26-27	0.3362995386242744
1101	28-29	0.4034588531204619
1101	30-31	0.45530569061266846
1101	32-33	0.4636676394190502
1101	34-35	0.49403151460493433
1101	36-37	0.4717922927672831
1101	38-39	0.4717254790148857
1101	40-41	0.5567632427651787
1101	42-43	0.5630804874421287
1101	44-45	0.5730468521663923
1101	46-47	0.5563750265213088
1101	48-49	0.5781852937846423
1101	50-51	0.6521634307535891
1101	52-53	0.6967541885439843
1101	54-55	0.8005049063275251
1101	56-57	0.5342456970146756
1101	58-59	0.4425826402570081
1101	60-61	0.5058428557402621
1101	62-63	0.5044871070656782
1101	64-65	0.5852776783958973
1101	66-67	0.5244513848375476
1101	68-69	0.5494001806592834
1101	70-71	0.44117099171787544
1101	72-73	0.5196127753979773
1101	74-75	0.5891368504706129
1101	76-77	0.5142226654786661
1101	78-79	0.5229295966597363
1101	80-81	0.5197834774655128
1101	82-83	0.5364758901868107
1101	84-85	0.5604568197615407
1101	86-87	0.6269720399250183
1101	88-89	0.6521618134601646
1101	90-91	0.5954478711641435
1101	92-93	0.6236943073849552
1101	94-95	0.6402221355803377
1101	96-97	0.6501395482975063
1101	98-99	0.7170388431536665
1101	100	0.6920158132989762
1102	1	1.5319124965364352
1102	2	3.1290423610663893
1102	3	3.263138713512795
1102	4	1.4035119313160749
1102	5	2.6558191464354977
1102	6	1.1979128544570585
1102	7	0.7091675976583218
1102	8	0.5246285241513888
1102	9	0.4129985422389879
1102	10-11	0.49411355099169185
1102	12-13	0.4680645024950749
1102	14-15	0.6078482026567755
1102	16-17	0.528128667754963
1102	18-19	0.4355856956083528
1102	20-21	0.34402953571250094
1102	22-23	0.33047384683788295
1102	24-25	0.40322500313180853
1102	26-27	0.4800811313408815
1102	28-29	0.5254023737532165
1102	30-31	0.5583792988746978
1102	32-33	0.5135404126670267
1102	34-35	0.5544304939006963
1102	36-37	0.5285849399476987
1102	38-39	0.5579477746855801
1102	40-41	0.6532293280352022
1102	42-43	0.6789796148104017
1102	44-45	0.6919978870103876
1102	46-47	0.6979003499144696
1102	48-49	0.5717799490395876
1102	50-51	0.6331509510638824
1102	52-53	0.7489628499825756
1102	54-55	0.7114403822547999
1102	56-57	0.6764963297076889
1102	58-59	0.7168796158101358
1102	60-61	0.6157660778972343
1102	62-63	0.5881306666786443
1102	64-65	0.6686606358913636
1102	66-67	0.6960953833521017
1102	68-69	0.6407692305209522
1102	70-71	0.6641997305849543
1102	72-73	0.645406422218386
1102	74-75	0.6688577013547601
1102	76-77	0.6626169397604329
1102	78-79	0.6352386322347456
1102	80-81	0.6119127429972941
1102	82-83	0.6470182574195142
1102	84-85	0.625524306729627
1102	86-87	0.6018677534881256
1102	88-89	0.5973759987071219
1102	90-91	0.5720561817429655
1102	92-93	0.6100305020772403
1102	94-95	0.6411586378822136
1102	96-97	0.7009662340231522
1102	98-99	0.6625930085330616
1102	100	0.6475714881337318
1103	1	1.2628547280279285
1103	2	2.944512589125452
1103	3	3.167051619953483
1103	4	1.3712466777543852
1103	5	2.576898002954067
1103	6	1.1823896352907752
1103	7	0.6509329465928104
1103	8	0.43478514535037505
1103	9	0.35475878573983977
1103	10-11	0.39590015961056935
1103	12-13	0.385928302767077
1103	14-15	0.5173772837172592
1103	16-17	0.36293234950383635
1103	18-19	0.2570347487661593
1103	20-21	0.19713478066940127
1103	22-23	0.13469855034475842
1103	24-25	0.1804394923787882
1103	26-27	0.22695063589740982
1103	28-29	0.24307270114755397
1103	30-31	0.2988874825389445
1103	32-33	0.27269736196424077
1103	34-35	0.3043542829519481
1103	36-37	0.3131821167878286
1103	38-39	0.28154666336113365
1103	40-41	0.38094553483239935
1103	42-43	0.39821082148688447
1103	44-45	0.4369650575899371
1103	46-47	0.35194122981322806
1103	48-49	0.3912635255348391
1103	50-51	0.4037467561563375
1103	52-53	0.46605018626408423
1103	54-55	0.41044797818483403
1103	56-57	0.3694142961601159
1103	58-59	0.44092770029919137
1103	60-61	0.44296050608389237
1103	62-63	0.3773481127292868
1103	64-65	0.39331753510591483
1103	66-67	0.3755821561781971
1103	68-69	0.33509227818419873
1103	70-71	0.3631282417011761
1103	72-73	0.41109002611170453
1103	74-75	0.3663860390641105
1103	76-77	0.3437558309083357
1103	78-79	0.34954098741080486
1103	80-81	0.35006272686906215
1103	82-83	0.3455261302122672
1103	84-85	0.34801824781509794
1103	86-87	0.41610361944921337
1103	88-89	0.3481461698389481
1103	90-91	0.34165884824177795
1103	92-93	0.398019943118225
1103	94-95	0.4366580235667925
1103	96-97	0.39827164544353266
1103	98-99	0.4757662525957471
1103	100	0.5280705795147682
1104	1	6.098054624024822
1104	2	5.112138806765238
1104	3	4.097766160524188
1104	4	1.8087159481257729
1104	5	2.8951972042051395
1104	6	1.2770887031850648
1104	7	0.697362363024034
1104	8	0.5104259917484129
1104	9	0.44096395021531265
1104	10-11	0.46527578353431664
1104	12-13	0.40374962112667134
1104	14-15	0.5096250508768279
1104	16-17	0.39205250788715773
1104	18-19	0.3499769138021378
1104	20-21	0.2953642764314566
1104	22-23	0.2730316190310944
1104	24-25	0.24437492821127904
1104	26-27	0.31049628782102445
1104	28-29	0.3693632190248266
1104	30-31	0.44718813249511413
1104	32-33	0.47083074658215907
1104	34-35	0.47739241046583203
1104	36-37	0.42797496144995506
1104	38-39	0.49837686816628235
1104	40-41	0.5513389373408799
1104	42-43	0.5836578830195194
1104	44-45	0.623250027369572
1104	46-47	0.49509880274509044
1104	48-49	0.6271859552852987
1104	50-51	0.6658116069017623
1104	52-53	0.6551103594001546
1104	54-55	0.7354156037382182
1104	56-57	0.7511234138923939
1104	58-59	0.8322679549423313
1104	60-61	0.9241711490685489
1104	62-63	0.9474303125088781
1104	64-65	0.8832742763924912
1104	66-67	0.955941178827338
1104	68-69	0.8651179088770036
1104	70-71	0.8855411485880325
1104	72-73	0.9119960677812706
1104	74-75	0.9724764838102473
1104	76-77	0.9422632060192058
1104	78-79	1.0043508780810164
1104	80-81	1.012984195666231
1104	82-83	1.0480427017536265
1104	84-85	1.007765929570649
1104	86-87	0.9411824916354696
1104	88-89	0.9111487724471239
1104	90-91	0.9313390070552785
1104	92-93	0.9532940994847472
1104	94-95	0.9650047853629857
1104	96-97	0.914803600461557
1104	98-99	0.9482971119119341
1104	100	1.0101881814713423
1105	1	1.7270033353595942
1105	2	3.155461191952316
1105	3	3.256834344512569
1105	4	1.433783317358774
1105	5	2.615017244955311
1105	6	1.1851011464963221
1105	7	0.6891912470323831
1105	8	0.4970454327791032
1105	9	0.39323019788854907
1105	10-11	0.43891232388703116
1105	12-13	0.40183900735667777
1105	14-15	0.47862846057229547
1105	16-17	0.3773361438291545
1105	18-19	0.3671064171237859
1105	20-21	0.35439564451363026
1105	22-23	0.30754889506947336
1105	24-25	0.3936645594879664
1105	26-27	0.4280296190048247
1105	28-29	0.46637714675342323
1105	30-31	0.4744113171841562
1105	32-33	0.517153000317073
1105	34-35	0.4934841547740163
1105	36-37	0.49400128970584234
1105	38-39	0.45584547466174996
1105	40-41	0.4369611711099495
1105	42-43	0.44673604001060596
1105	44-45	0.5328191557053259
1105	46-47	0.5429972888130195
1105	48-49	0.48323022800141047
1105	50-51	0.5270678958336106
1105	52-53	0.44801195815607286
1105	54-55	0.44283094730580075
1105	56-57	0.4328392862448993
1105	58-59	0.3577098078225518
1105	60-61	0.32204444530592724
1105	62-63	0.39312688185547273
1105	64-65	0.3795453753945708
1105	66-67	0.4139448948311468
1105	68-69	0.4354367702780664
1105	70-71	0.4951134857206654
1105	72-73	0.42974062150738135
1105	74-75	0.4002134480341759
1105	76-77	0.39412742399665035
1105	78-79	0.417759370634748
1105	80-81	0.43916738913528874
1105	82-83	0.410682757211557
1105	84-85	0.39299301628070893
1105	86-87	0.4886755841963897
1105	88-89	0.4405375074928912
1105	90-91	0.40651637446531197
1105	92-93	0.4753214669567889
1105	94-95	0.5508728408754813
1105	96-97	0.4943903043233995
1105	98-99	0.5154488752930817
1105	100	0.5800777616552999
1106	1	-0.7828299169661008
1106	2	1.792320665525434
1106	3	2.6998921293329374
1106	4	1.143444952197136
1106	5	2.4750754014431067
1106	6	1.114789397075313
1106	7	0.6504402329297605
1106	8	0.44337259060410616
1106	9	0.3615764131875494
1106	10-11	0.40391231624725066
1106	12-13	0.36085117527851196
1106	14-15	0.439454046832509
1106	16-17	0.3487579156322198
1106	18-19	0.2797291489944058
1106	20-21	0.30001202230261015
1106	22-23	0.26168066341030993
1106	24-25	0.2819934508878319
1106	26-27	0.25790704920664353
1106	28-29	0.37092413393197177
1106	30-31	0.35719814182813536
1106	32-33	0.4233756871348362
1106	34-35	0.43406197397775514
1106	36-37	0.4018402885793009
1106	38-39	0.46555555504999546
1106	40-41	0.4872210436291695
1106	42-43	0.4676257043664549
1106	44-45	0.570570840019208
1106	46-47	0.4532788963458785
1106	48-49	0.5096538983673611
1106	50-51	0.615660621523503
1106	52-53	0.6426641257498602
1106	54-55	0.6845964277498027
1106	56-57	0.6925958679373849
1106	58-59	0.6770512664084194
1106	60-61	0.6236493787692226
1106	62-63	0.627197764592573
1106	64-65	0.6696467047378434
1106	66-67	0.6758352354476997
1106	68-69	0.5759907159538713
1106	70-71	0.6296043629365471
1106	72-73	0.6102336136010216
1106	74-75	0.6512343796861302
1106	76-77	0.685486447961015
1106	78-79	0.7011533019627407
1106	80-81	0.6569559395896185
1106	82-83	0.6651694821299223
1106	84-85	0.6373027612109645
1106	86-87	0.672222413995506
1106	88-89	0.7180863223247123
1106	90-91	0.7098121779849293
1106	92-93	0.673473557357628
1106	94-95	0.6499282274914577
1106	96-97	0.7232014646863085
1106	98-99	0.6557055400408629
1106	100	0.6626553468011771
1107	1	-0.9812750403550936
1107	2	1.6932113867071656
1107	3	-0.6979477431042902
1107	4	-0.037628373846686713
1107	5	1.8807436557063681
1107	6	0.7208413306060351
1107	7	0.3029401093052968
1107	8	0.26488603477629624
1107	9	0.21272120840473718
1107	10-11	0.30804185098632075
1107	12-13	0.2979438179590588
1107	14-15	0.32894788558910193
1107	16-17	0.26894498308364945
1107	18-19	0.2810172651314389
1107	20-21	0.24761323489901343
1107	22-23	0.27638536327679475
1107	24-25	0.17185169953227586
1107	26-27	0.22672431347116628
1107	28-29	0.17805196188442807
1107	30-31	0.19020592802547043
1107	32-33	0.23647833182773326
1107	34-35	0.23568834168889907
1107	36-37	0.3299434122249352
1107	38-39	0.3769407410140744
1107	40-41	0.391482155373545
1107	42-43	0.40410904455108465
1107	44-45	0.399631774228709
1107	46-47	0.4557469183555156
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2216	88-89	0.2036457609172686
2216	90-91	0.18895856870168615
2216	92-93	0.14239412138678276
2216	94-95	0.0908057740834387
2216	96-97	0.09139325800758868
2216	98-99	0.07152793589779094
2216	100	0.0773187019575694
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1444.0
3	368.0
4	445.0
5	1212.0
6	3661.0
7	6369.0
8	7610.0
9	10639.0
10	7596.0
11	6648.0
12	6342.0
13	8787.0
14	9326.0
15	10149.0
16	11854.0
17	13731.0
18	19656.0
19	35446.0
20	70845.0
21	84946.0
22	37615.0
23	24031.0
24	23918.0
25	25561.0
26	26462.0
27	28910.0
28	33976.0
29	39879.0
30	46779.0
31	57793.0
32	74808.0
33	104884.0
34	156170.0
35	307837.0
36	459235.0
37	717664.0
38	670988.0
39	155403.0
40	652.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.051531743495898	41.13631742527667	23.775004599103415	21.03714623212401
2	15.574320087869983	40.32026614814915	25.022851332629287	19.082562431351572
3	18.6790475117094	24.843806701936625	18.20423559784029	38.272910188513684
4	33.86043009524604	26.096743481690904	20.857592021365473	19.18523440169759
5	17.95932439173773	39.61739825130358	21.051073091183667	21.372204265775025
6	37.72278609996047	22.668519666822597	22.646732107911575	16.96196212530536
7	19.487125718830146	22.622391483539726	39.336536997241915	18.55394580038821
8	36.43853604577418	22.599957276307176	22.414227050140514	18.547279627778135
9	36.05949168474265	24.23321697623215	22.399693743033605	17.307597595991588
10-11	17.305845139001566	42.46658019197864	22.284424373776112	17.943150295243683
12-13	17.29008813347921	24.040552459044626	31.130963225898654	27.538396181577507
14-15	17.638766644942244	32.93026822562823	22.07783084499548	27.353134284434045
16-17	26.998050240524723	23.60493093053351	31.341817038051584	18.055201790890184
18-19	17.77498996114078	23.838853723925784	31.430029075678647	26.956127239254794
20-21	27.11480013379103	33.201068152750196	22.16415445914192	17.51997725431686
22-23	26.669585413998327	24.0746045112473	31.479989207282124	17.77582086747225
24-25	17.619127040743717	33.205509724776626	22.031209446105755	27.1441537883739
26-27	18.120050911920114	27.677562875088284	36.37482956970363	17.827556643287974
28-29	25.998620997637506	23.85708834105472	32.40797561305024	17.736315048257527
30-31	24.014824577544562	24.872591844609033	32.68817233541181	18.424411242434598
32-33	20.110289974223775	26.5625948932799	33.736851662673786	19.59026346982254
34-35	19.768878116314195	26.248406548267045	34.10032030683709	19.88239502858167
36-37	19.772639855887604	26.452658431931702	34.07442624407073	19.700275468109965
38-39	20.31241171620228	26.11073292283539	33.784258645731455	19.792596715230875
40-41	19.624995354478237	27.018702039708863	33.70196870413963	19.654333901673265
42-43	19.671511492202942	26.578692647948166	34.03407981022558	19.715716049623317
44-45	19.701952388160763	26.855466714043434	33.49827579382525	19.944305103970553
46-47	20.116710614057908	26.456676997098477	33.723526946594475	19.70308544224914
48-49	19.71060892139596	26.79265019538385	33.54728415999449	19.949456723225705
50-51	19.945770520591523	27.109618299760186	33.074181202239885	19.870429977408413
52-53	20.13530486878168	27.07899688092682	32.721534113862035	20.06416413642946
54-55	20.62055710607713	27.819907246681584	30.87709868055096	20.682436966690325
56-57	20.957844647105016	28.561408056890798	29.367764883118674	21.112982412885515
58-59	21.48048472960344	29.185630215259128	28.305927021043686	21.02795803409375
60-61	21.385821837366553	29.362447082597225	28.105874991199947	21.145856088836275
62-63	20.70289862516686	29.537429773286384	27.84423855418629	21.915433047360466
64-65	20.86324218441951	29.45688336401644	28.686119543551424	20.993754908012626
66-67	20.92653019355437	29.045619332745858	28.909562177258934	21.118288296440834
68-69	20.976169908561022	29.151547948280765	28.870384353097123	21.00189779006109
70-71	20.15470569448813	29.619302890738236	29.17339323110466	21.052598183668973
72-73	20.215438207302245	29.31081498169053	29.184033304060144	21.289713506947084
74-75	20.38661729356715	29.543337101630257	28.687017685816496	21.383027918986098
76-77	20.923219537442993	29.242970010646225	28.607006625676117	21.22680382623467
78-79	20.968301885196194	29.505678733175227	27.96976639979889	21.556252981829687
80-81	21.00008611273517	29.961264376672208	27.733754530360784	21.30489498023184
82-83	20.86641497576002	30.235722281896837	27.521690589388797	21.376172152954346
84-85	20.990833804562566	30.380650992799296	27.04365307476886	21.584862127869282
86-87	20.97744526742804	30.502837470442383	27.02352946153023	21.49618780059934
88-89	21.546005470687287	30.181056000367413	26.901000381008323	21.37193814793698
90-91	21.24053181663125	30.603205124912492	26.748054242806692	21.40820881564957
92-93	20.850385797363398	30.704678063075765	26.779113975874708	21.66582216368613
94-95	20.9139123632517	30.947982544319792	26.539979133675907	21.5981259587526
96-97	20.87704832093053	30.441433876772457	26.64414834295735	22.03736945933966
98-99	21.392868192882332	30.42126537636904	26.11341953043609	22.072446900312542
100	20.99141326289665	30.93742248021612	25.88502854843081	22.18613570845642
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	85785.0
1	47050.5
2	6233.0
3	3539.0
4	2927.5
5	2769.5
6	2757.0
7	3061.0
8	2974.0
9	2663.0
10	2657.5
11	4119.5
12	29812.0
13	33189.0
14	10832.5
15	9676.0
16	10653.0
17	11923.0
18	13248.5
19	14755.5
20	16335.0
21	18133.0
22	20442.5
23	23153.5
24	25796.5
25	28396.0
26	32189.5
27	36514.5
28	39447.5
29	41966.5
30	44966.5
31	47935.0
32	51618.5
33	55754.0
34	60013.0
35	64259.5
36	67797.5
37	70771.0
38	72768.5
39	73825.0
40	75280.5
41	77394.0
42	79028.5
43	81878.5
44	86667.0
45	90404.5
46	93850.0
47	98925.0
48	103243.5
49	108574.5
50	121422.0
51	124576.5
52	113101.0
53	107705.0
54	107285.5
55	106715.5
56	99872.0
57	88619.0
58	78846.5
59	66550.0
60	54381.0
61	44690.0
62	36555.0
63	29193.5
64	22114.0
65	16564.0
66	12636.5
67	9438.5
68	6967.5
69	5086.5
70	3718.5
71	2788.0
72	2082.5
73	1363.5
74	843.5
75	551.5
76	362.5
77	227.0
78	137.5
79	84.5
80	40.5
81	24.5
82	11.0
83	4.5
84	6.0
85	4.5
86	2.5
87	1.5
88	1.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1404080626316042
2	0.006163814240767649
3	0.0035653435314244243
4	0.0
5	0.0026589002607232994
6	0.01241827280860541
7	0.002870403690553562
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.532216353505624E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	7.704767800959561E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	6.0429551380074994E-5
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	3.0214775690037497E-5
64-65	0.0
66-67	1.5107387845018748E-5
68-69	0.0
70-71	0.0
72-73	3.7768469612546867E-4
74-75	2.870403690553562E-4
76-77	4.381142475055437E-4
78-79	3.0214775690037496E-4
80-81	7.251546165608999E-4
82-83	7.251546165608999E-4
84-85	9.819802099262187E-4
86-87	3.0214775690037497E-5
88-89	0.0
90-91	6.0429551380074994E-5
92-93	0.0
94-95	0.0
96-97	0.001148161476221425
98-99	4.5322163535056243E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3309639.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.19871840207533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.19973144854691	55.98111004001856
2	8.753806144560325	11.239662712419703
3	2.0101795892614733	3.871528601657903
4	0.680084480017935	1.7464220808917292
5	0.3373204157068233	1.082776918961667
6	0.18484311142306145	0.7120014515287534
7	0.12328078832759848	0.5540128029960544
8	0.0884179619255919	0.45410558714771937
9	0.06386077239400105	0.36898017694753493
>10	0.5075836590117675	6.635977846216343
>50	0.03003924933517349	1.3038934848430839
>100	0.017839582444734263	2.2812095661521243
>500	0.0020712979684991174	0.9351746233864212
>1k	4.707495380786395E-4	0.6308439465472097
>5k	2.824497228471837E-4	1.1786089427706539
>10k+	1.8829981523145578E-4	11.023691217514603
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	254409	7.686910868526748	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	80086	2.419780525912343	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20083	0.6068033401830231	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	10185	0.3077374904030319	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8573	0.2590312719906914	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7076	0.2137997527827053	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6803	0.20555111901932507	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5892	0.1780254583657009	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5648	0.17065305309733175	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	5007	0.15128538188001775	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA	3426	0.10351582151406846	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001903530868472362	0.0	0.0	0.0	0.0
2	0.001903530868472362	0.0	0.0	0.0	0.0
3	0.001903530868472362	0.0	0.0	0.0	0.0
4	0.001903530868472362	0.0	0.0	0.0	3.0214775690037494E-5
5	0.001903530868472362	0.0	0.0	0.0	3.0214775690037494E-5
6	0.001903530868472362	0.0	0.0	0.0	3.0214775690037494E-5
7	0.001903530868472362	0.0	0.0	0.0	3.0214775690037494E-5
8	0.001903530868472362	0.0	0.0	0.0	3.0214775690037494E-5
9	0.001903530868472362	0.0	0.0	0.0	3.0214775690037494E-5
10-11	0.0019186382563173808	0.0	0.0	3.0214775690037494E-5	3.0214775690037494E-5
12-13	0.0019337456441623996	0.0	0.0	3.0214775690037494E-5	3.0214775690037494E-5
14-15	0.0019337456441623996	0.0	0.0	3.0214775690037494E-5	3.0214775690037494E-5
16-17	0.0019337456441623996	0.0	0.0	3.0214775690037494E-5	3.0214775690037494E-5
18-19	0.0019337456441623996	0.0	0.0	3.0214775690037494E-5	3.0214775690037494E-5
20-21	0.0019337456441623996	0.0	0.0	3.0214775690037494E-5	3.0214775690037494E-5
22-23	0.0019337456441623996	0.0	0.0	4.532216353505624E-5	3.0214775690037494E-5
24-25	0.0019488530320074184	0.0	0.0	6.042955138007499E-5	3.0214775690037494E-5
26-27	0.001963960419852437	0.0	0.0	6.042955138007499E-5	3.0214775690037494E-5
28-29	0.001979067807697456	0.0	0.0	6.042955138007499E-5	3.0214775690037494E-5
30-31	0.0019941751955424745	0.0	0.0	6.042955138007499E-5	3.0214775690037494E-5
32-33	0.0020697121347675682	0.0	1.5107387845018747E-5	6.042955138007499E-5	3.0214775690037494E-5
34-35	0.002432289443048018	0.0	3.0214775690037494E-5	6.042955138007499E-5	3.0214775690037494E-5
36-37	0.0029459406297786556	0.0	3.0214775690037494E-5	6.042955138007499E-5	3.0214775690037494E-5
38-39	0.003822169124789743	0.0	3.0214775690037494E-5	6.042955138007499E-5	3.0214775690037494E-5
40-41	0.005499089175586824	0.0	3.0214775690037494E-5	6.042955138007499E-5	3.0214775690037494E-5
42-43	0.007130687062848849	0.0	3.0214775690037494E-5	6.042955138007499E-5	3.0214775690037494E-5
44-45	0.008807607113645928	0.0	3.0214775690037494E-5	9.064432707011248E-5	3.0214775690037494E-5
46-47	0.01098307096332863	0.0	3.0214775690037494E-5	9.064432707011248E-5	3.0214775690037494E-5
48-49	0.01350600473344676	0.0	3.0214775690037494E-5	9.064432707011248E-5	3.0214775690037494E-5
50-51	0.017177099979786316	0.0	3.0214775690037494E-5	9.064432707011248E-5	3.0214775690037494E-5
52-53	0.02169420894544692	0.0	3.0214775690037494E-5	9.064432707011248E-5	3.0214775690037494E-5
54-55	0.026785398649218237	3.0214775690037494E-5	3.0214775690037494E-5	9.064432707011248E-5	3.0214775690037494E-5
56-57	0.03341754191318147	3.0214775690037494E-5	3.0214775690037494E-5	9.064432707011248E-5	3.0214775690037494E-5
58-59	0.04143956485888642	3.0214775690037494E-5	3.0214775690037494E-5	9.064432707011248E-5	3.0214775690037494E-5
60-61	0.05180323292056928	3.0214775690037494E-5	3.0214775690037494E-5	9.064432707011248E-5	3.0214775690037494E-5
62-63	0.06576245928936661	3.0214775690037494E-5	3.0214775690037494E-5	9.064432707011248E-5	6.042955138007499E-5
64-65	0.08139860570896101	3.0214775690037494E-5	3.0214775690037494E-5	9.064432707011248E-5	6.042955138007499E-5
66-67	0.1010533172953304	3.0214775690037494E-5	3.0214775690037494E-5	9.064432707011248E-5	6.042955138007499E-5
68-69	0.12551217821641575	3.0214775690037494E-5	3.0214775690037494E-5	9.064432707011248E-5	6.042955138007499E-5
70-71	0.15871821669976693	3.0214775690037494E-5	3.0214775690037494E-5	9.064432707011248E-5	6.042955138007499E-5
72-73	0.19766506256422528	3.0214775690037494E-5	3.0214775690037494E-5	9.064432707011248E-5	6.042955138007499E-5
74-75	0.24268507834238115	3.0214775690037494E-5	3.0214775690037494E-5	9.064432707011248E-5	6.042955138007499E-5
76-77	0.2972982854021239	3.0214775690037494E-5	3.0214775690037494E-5	9.064432707011248E-5	6.042955138007499E-5
78-79	0.3664750143444647	3.0214775690037494E-5	3.0214775690037494E-5	9.064432707011248E-5	6.042955138007499E-5
80-81	0.4532065279627174	3.0214775690037494E-5	3.0214775690037494E-5	9.064432707011248E-5	6.042955138007499E-5
82-83	0.5610732771761513	3.0214775690037494E-5	3.0214775690037494E-5	9.064432707011248E-5	6.042955138007499E-5
84-85	0.6887307044665596	3.0214775690037494E-5	3.0214775690037494E-5	9.064432707011248E-5	6.042955138007499E-5
86-87	0.836526279754378	3.0214775690037494E-5	3.0214775690037494E-5	9.064432707011248E-5	6.042955138007499E-5
88	0.9646973582315171	3.0214775690037494E-5	3.0214775690037494E-5	9.064432707011248E-5	6.042955138007499E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	4840	0.0	85.88057	1
AACGAGT	60840	0.0	79.26108	1
ACGAGTG	66970	0.0	78.48075	2
CGAGTGG	70145	0.0	77.215866	3
GTGGAAT	74400	0.0	77.14333	6
TGGAATC	75005	0.0	76.48678	7
GAATCAC	75585	0.0	75.9586	9
GAGTGGA	73355	0.0	75.87888	4
GGAATCA	75650	0.0	75.868484	8
AGTGGAA	73705	0.0	75.29534	5
AACGTGT	2380	0.0	65.86381	1
CGTGTGG	2760	0.0	64.21074	3
ATCGAGT	1795	0.0	62.6777	1
TCGAGTG	1880	0.0	62.51494	2
ACGTGTG	2740	0.0	60.05085	2
ATGAGTG	3205	0.0	49.871513	2
AATGAGT	3040	0.0	48.622314	1
TGAGTGG	3570	0.0	48.061802	3
GCGAGTG	1210	0.0	43.903053	2
GTGTGGA	4380	0.0	43.78732	4
>>END_MODULE