FastQCFastQC Report
Thu 26 May 2016
SRR2132753_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132753_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2853408
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1819026.374903273559196No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA294471.0319940225863249No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT149840.5251264452892822No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG69800.24461976695936927No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT59170.20736606892529916No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50690.17764722044656775No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48230.1690259507227848No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41960.14705222667070394No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC34250.12003190570714037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT38050.087.241411
AACGAGT451600.081.742951
ACGAGTG501300.080.5466462
CGAGTGG524150.079.5872653
GTGGAAT556150.079.313186
GAATCAC561950.078.448739
TGGAATC563100.078.2996147
GGAATCA564850.078.070938
GAGTGGA549150.077.871644
AGTGGAA554100.076.997825
ATCGAGT13550.071.584021
ACGTGTG19700.067.056782
CGTGTGG20450.066.892813
AACGTGT19000.066.167661
TCGAGTG15250.065.353462
AGCGAGT9300.046.579311
GCGAGTG10050.044.906352
GTGTGGA33450.044.689254
ATGAGTG25000.043.438472
TGAGTGG27500.042.2225043