FastQCFastQC Report
Thu 26 May 2016
SRR2132752_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132752_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2620630
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2294328.754841393100133No Hit
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2189188.353640155229849No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG297231.134192923075749No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA188940.7209716747499647No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT177600.6776996371101606No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC113030.4313085021540622No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA74810.2854657086273148No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT69660.26581394550165416No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65640.25047412263463364No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT61420.23437112450059716No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55950.21349828094771103No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55000.2098731984293853No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50820.19392283534875202No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48340.18445946203775426No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44260.16889068659062897No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35260.13454779957491136No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29970.11436181376233959No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA28790.1098590796869455No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG28230.10772218893930086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT44400.085.770331
AACGTGT16500.080.737261
CGTGTGG19450.075.1634753
TCGAGTG14900.072.253142
ATCGAGT15050.069.7493061
ACGTGTG20400.067.982852
GTGGAAT799150.064.067176
TGGAATC806150.063.490957
GGAATCA814000.062.8416188
GAATCAC815400.062.7394839
ACGAGTG761650.061.723722
AGTGGAA809650.061.512235
CGAGTGG787700.061.508843
GAGTGGA815600.061.0519524
AACGAGT705750.061.0497781
ATGAGTG23750.060.7690282
TGAGTGG26200.059.3871963
AATGAGT22800.058.221821
GCGAGTG8600.056.8515662
AGCGAGT8900.051.833151
Produced by FastQC (version 0.11.5)