##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132752_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2620630
Sequences flagged as poor quality	0
Sequence length	100
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.072214696466116	31.0	25.0	33.0	16.0	34.0
2	28.321726455089042	31.0	26.0	34.0	16.0	34.0
3	29.345015893124934	31.0	28.0	34.0	16.0	34.0
4	34.778562788337155	35.0	35.0	37.0	32.0	37.0
5	33.91290109630127	35.0	35.0	37.0	28.0	37.0
6	35.24207003659426	37.0	35.0	37.0	32.0	37.0
7	35.605897436875864	37.0	35.0	37.0	33.0	37.0
8	35.87813464701236	37.0	35.0	37.0	35.0	37.0
9	37.83090363767491	39.0	38.0	39.0	35.0	39.0
10-11	37.81863101620603	39.0	38.0	39.0	35.0	39.0
12-13	37.75952976955923	39.0	38.0	39.0	35.0	39.0
14-15	39.121570385746935	41.0	39.0	41.0	36.0	41.0
16-17	38.89978783727577	40.5	39.0	41.0	36.0	41.0
18-19	38.313824919961995	40.0	37.0	41.0	34.5	41.0
20-21	37.99510232272392	40.0	37.0	41.0	34.0	41.0
22-23	37.701494297172815	40.0	36.5	41.0	33.5	41.0
24-25	37.31295585412668	40.0	36.0	41.0	33.0	41.0
26-27	36.93049362176271	40.0	35.5	41.0	32.5	41.0
28-29	36.72245318873706	40.0	35.0	41.0	32.0	41.0
30-31	36.56718346351832	40.0	35.0	41.0	31.5	41.0
32-33	36.39827198040166	40.0	35.0	41.0	31.0	41.0
34-35	36.27006177903786	40.0	35.0	41.0	30.5	41.0
36-37	36.039616046523165	39.5	35.0	41.0	30.0	41.0
38-39	35.48201787356476	38.0	35.0	41.0	30.0	41.0
40-41	35.206752956350186	38.0	35.0	41.0	29.5	41.0
42-43	35.019569340196824	38.0	35.0	41.0	27.5	41.0
44-45	34.875847219943296	38.0	35.0	40.0	26.5	41.0
46-47	34.71260555667912	37.5	35.0	40.0	25.5	41.0
48-49	34.51615661119654	37.0	35.0	40.0	25.5	41.0
50-51	34.211654640296416	37.0	35.0	40.0	23.5	41.0
52-53	33.67136089413614	36.5	34.5	40.0	20.0	41.0
54-55	32.7383524190748	36.0	33.0	40.0	4.5	41.0
56-57	32.01324490675906	35.5	33.0	40.0	2.0	41.0
58-59	31.6623138329333	35.0	33.0	40.0	2.0	41.0
60-61	31.4156208621591	35.0	33.0	39.5	2.0	41.0
62-63	31.174889053395557	35.0	33.0	39.0	2.0	41.0
64-65	30.90639960620156	35.0	33.0	39.0	2.0	41.0
66-67	30.583782907163545	35.0	32.0	38.5	2.0	40.5
68-69	30.30546090062313	35.0	32.0	37.0	2.0	40.0
70-71	30.040097610116653	35.0	31.5	37.0	2.0	40.0
72-73	29.78349919675803	35.0	31.0	36.0	2.0	39.0
74-75	29.514102715759186	35.0	31.0	36.0	2.0	39.0
76-77	29.23119173633821	35.0	31.0	35.5	2.0	39.0
78-79	28.947255240152177	35.0	31.0	35.0	2.0	37.5
80-81	28.6715972876751	35.0	30.0	35.0	2.0	37.0
82-83	28.402784444961707	35.0	30.0	35.0	2.0	36.5
84-85	28.153980149811304	35.0	29.0	35.0	2.0	36.0
86-87	27.99109221828339	35.0	29.0	35.0	2.0	36.0
88-89	27.770187130575472	34.5	29.0	35.0	2.0	36.0
90-91	27.579157874251614	34.0	29.0	35.0	2.0	35.0
92-93	27.42774065778076	34.0	29.0	35.0	2.0	35.0
94-95	27.218317732758916	34.0	28.5	35.0	2.0	35.0
96-97	26.977853989307913	34.0	27.0	35.0	2.0	35.0
98-99	26.800174385548516	34.0	27.0	35.0	2.0	35.0
100	26.71124653232238	34.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.13707097198259888
1101	2	2.121736226039257
1101	3	1.1498921377104203
1101	4	0.6484304906411396
1101	5	2.1255456312059806
1101	6	0.9831540281896594
1101	7	0.5523153236379343
1101	8	0.38725970707619695
1101	9	0.383530826048208
1101	10-11	0.4976465131047547
1101	12-13	0.4412087855841662
1101	14-15	0.5514252816899585
1101	16-17	0.49306495899042346
1101	18-19	0.46289240840768286
1101	20-21	0.4493022484570304
1101	22-23	0.3325317880737799
1101	24-25	0.10185723168677896
1101	26-27	0.023318311625196486
1101	28-29	0.1018489857870577
1101	30-31	0.15607799039834447
1101	32-33	0.1525411563311465
1101	34-35	0.130502470548592
1101	36-37	0.18094490834292287
1101	38-39	0.17791434689313945
1101	40-41	0.23028797271475554
1101	42-43	0.20534505469475306
1101	44-45	0.30150681481232766
1101	46-47	0.18225063911199157
1101	48-49	0.20950276966280512
1101	50-51	0.23165813223145904
1101	52-53	0.28116656403783225
1101	54-55	0.3449077722191305
1101	56-57	0.3491905189088129
1101	58-59	0.37030451447774126
1101	60-61	0.399355027490202
1101	62-63	0.39790876539101205
1101	64-65	0.4873447133194624
1101	66-67	0.4370208526041566
1101	68-69	0.5104281197645584
1101	70-71	0.4690236416240374
1101	72-73	0.538439485164897
1101	74-75	0.5519389508780002
1101	76-77	0.5633213279334051
1101	78-79	0.5899975956549177
1101	80-81	0.6881617680165029
1101	82-83	0.6364334311615885
1101	84-85	0.6362330919868171
1101	86-87	0.6769832958485829
1101	88-89	0.6346478729085057
1101	90-91	0.6953080611523177
1101	92-93	0.7096404418535052
1101	94-95	0.7910855032798807
1101	96-97	0.7586653606454661
1101	98-99	0.768126220794418
1101	100	0.8019541729160302
1102	1	1.4593466020233556
1102	2	2.875312651393422
1102	3	3.059043253595828
1102	4	1.3722699296272225
1102	5	2.5269146436527805
1102	6	1.1753979921249567
1102	7	0.7066543572705157
1102	8	0.4792024869389806
1102	9	0.4375236455957676
1102	10-11	0.4553656662536554
1102	12-13	0.3947388967365839
1102	14-15	0.49873149461420496
1102	16-17	0.3275950066004967
1102	18-19	0.15503530498158113
1102	20-21	0.04169799689002218
1102	22-23	-0.07230428516903231
1102	24-25	0.016707562765773787
1102	26-27	0.12054194450984568
1102	28-29	0.17977158786801795
1102	30-31	0.14900402560080295
1102	32-33	0.19396468177752269
1102	34-35	0.2183513551635201
1102	36-37	0.1978754425439604
1102	38-39	0.2108374568125626
1102	40-41	0.24807299309750874
1102	42-43	0.24126499533519308
1102	44-45	0.3200176522855003
1102	46-47	0.4350668077550566
1102	48-49	0.3579031785353166
1102	50-51	0.47056219512271014
1102	52-53	0.516471394707466
1102	54-55	0.5170100617284277
1102	56-57	0.36556284773264025
1102	58-59	0.48427764423539443
1102	60-61	0.48690062663203193
1102	62-63	0.44813599183743946
1102	64-65	0.4103525647190871
1102	66-67	0.4072877855149457
1102	68-69	0.40476677981481757
1102	70-71	0.35407419448418764
1102	72-73	0.34642792612746476
1102	74-75	0.4088255658068505
1102	76-77	0.3712760797755088
1102	78-79	0.40648028136494574
1102	80-81	0.4408764523536135
1102	82-83	0.3904222980747214
1102	84-85	0.2365954001318613
1102	86-87	0.33703972345758615
1102	88-89	0.3826425780461413
1102	90-91	0.39417960903898575
1102	92-93	0.43113715274465036
1102	94-95	0.5067956907865181
1102	96-97	0.4643385251429386
1102	98-99	0.5187896212841778
1102	100	0.4059510701813913
1103	1	1.2414568196894997
1103	2	2.934296189149702
1103	3	3.106465422942666
1103	4	1.332852879637315
1103	5	2.499773875626758
1103	6	1.1902584491431654
1103	7	0.6840094113408242
1103	8	0.48257023045627534
1103	9	0.363446288597018
1103	10-11	0.4198049216476605
1103	12-13	0.38848813054673315
1103	14-15	0.47550722588836436
1103	16-17	0.19174214556565516
1103	18-19	0.14472581424092112
1103	20-21	0.06825898549492138
1103	22-23	-0.06819232351779903
1103	24-25	0.049205616200353575
1103	26-27	0.14296929520717327
1103	28-29	0.15489385323503058
1103	30-31	0.2157123955581426
1103	32-33	0.2729390730668726
1103	34-35	0.3278064844533901
1103	36-37	0.26509987930779744
1103	38-39	0.2977462174474894
1103	40-41	0.4107844279217119
1103	42-43	0.3834698590036325
1103	44-45	0.4972403603208875
1103	46-47	0.4434939560332154
1103	48-49	0.48603131481026196
1103	50-51	0.5642953772218462
1103	52-53	0.5472198223865092
1103	54-55	0.4834773229530498
1103	56-57	0.45896879088401477
1103	58-59	0.42997053718454836
1103	60-61	0.46530398421161223
1103	62-63	0.4368046549759512
1103	64-65	0.4643343667632429
1103	66-67	0.5518473836161988
1103	68-69	0.5486926630269089
1103	70-71	0.5343827204031335
1103	72-73	0.5861649305537178
1103	74-75	0.6177092531104975
1103	76-77	0.5893212489034916
1103	78-79	0.5700210545911197
1103	80-81	0.46334613487247367
1103	82-83	0.45216659045051344
1103	84-85	0.47077218963709555
1103	86-87	0.5125808697195069
1103	88-89	0.5706003781623714
1103	90-91	0.5160543736756722
1103	92-93	0.6415032327502885
1103	94-95	0.6641426147833869
1103	96-97	0.6028845065798976
1103	98-99	0.664030288970892
1103	100	0.7538192063424454
1104	1	5.840559907072041
1104	2	4.88234250308604
1104	3	3.955928622763235
1104	4	1.7549055855307358
1104	5	2.705716200274537
1104	6	1.2437982600090791
1104	7	0.6870439299312068
1104	8	0.529640604092144
1104	9	0.42700414790009233
1104	10-11	0.4343070173256294
1104	12-13	0.29490650929071904
1104	14-15	0.3796166241369221
1104	16-17	0.19875492390868743
1104	18-19	-0.014245179298001176
1104	20-21	-0.08033952148025492
1104	22-23	-0.21195977331510107
1104	24-25	-0.2050259757658992
1104	26-27	-0.16000550899296684
1104	28-29	-0.09658126140153911
1104	30-31	-0.07615834857931247
1104	32-33	-0.08062448737165795
1104	34-35	-0.036263166791052015
1104	36-37	-0.011924751124297472
1104	38-39	-0.002342477198830295
1104	40-41	0.019145412346645685
1104	42-43	0.18731757864230048
1104	44-45	0.17494937461458449
1104	46-47	0.11102877515577347
1104	48-49	0.12284639188907676
1104	50-51	0.13614742914572275
1104	52-53	0.24374830959650495
1104	54-55	0.21214768940832585
1104	56-57	0.03824593725421721
1104	58-59	0.22319114842963472
1104	60-61	0.20986206161458654
1104	62-63	0.1526646959176574
1104	64-65	0.18738278019481314
1104	66-67	0.171016590139061
1104	68-69	0.13546387251980363
1104	70-71	0.17688335437847513
1104	72-73	0.1171370247027177
1104	74-75	0.20859279086003824
1104	76-77	0.21689963023083791
1104	78-79	0.24903423199299723
1104	80-81	0.2709056279645665
1104	82-83	0.22104796494619805
1104	84-85	0.2106607729843759
1104	86-87	0.23633217558490927
1104	88-89	0.2737042574724491
1104	90-91	0.2370742723510375
1104	92-93	0.3048180664527287
1104	94-95	0.2852351033775058
1104	96-97	0.23760086219456866
1104	98-99	0.2861383761439953
1104	100	0.30295482300494925
1105	1	1.2533953800741564
1105	2	2.8238345280057935
1105	3	3.026375534440316
1105	4	1.2989144591831803
1105	5	2.4167378595330575
1105	6	1.119375841580009
1105	7	0.6747908558790243
1105	8	0.44823435673767165
1105	9	0.31703487431246913
1105	10-11	0.35239897645490714
1105	12-13	0.3599337688027262
1105	14-15	0.366589594810975
1105	16-17	0.24329648075658383
1105	18-19	0.04976382940230195
1105	20-21	-0.006591042280447823
1105	22-23	-0.17088199259897152
1105	24-25	-0.09733125085638505
1105	26-27	-0.007262243446945149
1105	28-29	0.017820051263697678
1105	30-31	-0.008829732380498001
1105	32-33	0.027150107263032908
1105	34-35	0.04648907439879224
1105	36-37	0.005533762978323864
1105	38-39	0.08330895942825123
1105	40-41	0.14220248679873748
1105	42-43	0.11021447020329589
1105	44-45	0.1721943982015972
1105	46-47	0.15558937979932352
1105	48-49	0.16221380149368514
1105	50-51	0.15679735605861822
1105	52-53	0.30730314426970295
1105	54-55	0.2960390694039887
1105	56-57	0.42034280325690077
1105	58-59	0.49215695793877856
1105	60-61	0.4010285103234068
1105	62-63	0.39311414125391053
1105	64-65	0.3759314671667653
1105	66-67	0.39756270676720007
1105	68-69	0.430804832093326
1105	70-71	0.41554366747989135
1105	72-73	0.4276353432560356
1105	74-75	0.39492679365451977
1105	76-77	0.3797805055183687
1105	78-79	0.4775935192144054
1105	80-81	0.4319671364209796
1105	82-83	0.3707184380813473
1105	84-85	0.3789896201754992
1105	86-87	0.3695032264024114
1105	88-89	0.34765359517354355
1105	90-91	0.2639700621633523
1105	92-93	0.3448890351127538
1105	94-95	0.3825490316094431
1105	96-97	0.3545619869538008
1105	98-99	0.27388681382162616
1105	100	0.3112140076706069
1106	1	-0.936994625621594
1106	2	1.6885735545022555
1106	3	2.5476506370760816
1106	4	1.1452473359039033
1106	5	2.3170544431452242
1106	6	1.0912628289370758
1106	7	0.5981664956300463
1106	8	0.40417855472925623
1106	9	0.2553256188223969
1106	10-11	0.3716059505831453
1106	12-13	0.3241073544094206
1106	14-15	0.29024147328533445
1106	16-17	0.2683725516696782
1106	18-19	0.20315818448631973
1106	20-21	0.16640735617292535
1106	22-23	0.06080436250955046
1106	24-25	0.10410559880238424
1106	26-27	0.0836226697592295
1106	28-29	0.18556223287847473
1106	30-31	0.16288556591138814
1106	32-33	0.17762411504926234
1106	34-35	0.23678208393866385
1106	36-37	0.2802396612428595
1106	38-39	0.3344402750237876
1106	40-41	0.26186213517300416
1106	42-43	0.26612087516492267
1106	44-45	0.30869945394439924
1106	46-47	0.2590653433301995
1106	48-49	0.29085083835681047
1106	50-51	0.31054710852831136
1106	52-53	0.30663042323278944
1106	54-55	0.3349194209072408
1106	56-57	0.2302537637970019
1106	58-59	0.2336888949629845
1106	60-61	0.1759684140468778
1106	62-63	0.19073129199119165
1106	64-65	0.19890059579882546
1106	66-67	0.27542405508776824
1106	68-69	0.22182001769512638
1106	70-71	0.27625591170707864
1106	72-73	0.23464310498999552
1106	74-75	0.31010545439815473
1106	76-77	0.30333980255270276
1106	78-79	0.33778179025856403
1106	80-81	0.27653392265074217
1106	82-83	0.3200358413505846
1106	84-85	0.2306129851995138
1106	86-87	0.203288893733923
1106	88-89	0.25210268456821794
1106	90-91	0.33909836962764217
1106	92-93	0.40452385900387
1106	94-95	0.4392396228036901
1106	96-97	0.4822881377911159
1106	98-99	0.44924591140266656
1106	100	0.42178824736977205
1107	1	-0.9937043503281835
1107	2	1.638282060120396
1107	3	-0.8148517654972274
1107	4	-0.09511685940938008
1107	5	1.7983393741518867
1107	6	0.7374228609321278
1107	7	0.358002376449555
1107	8	0.32312389598568103
1107	9	0.2963131714849325
1107	10-11	0.32544752476523797
1107	12-13	0.2603587457603709
1107	14-15	0.3425320960483944
1107	16-17	0.31570469143492375
1107	18-19	0.36318530746434163
1107	20-21	0.30755831059015293
1107	22-23	0.3527795534973919
1107	24-25	0.1693005967384451
1107	26-27	0.1422155568572876
1107	28-29	0.1438090691110432
1107	30-31	0.1439125448688614
1107	32-33	0.13853185141562818
1107	34-35	0.11378585684876441
1107	36-37	0.22802466265360977
1107	38-39	0.16667394809928027
1107	40-41	0.23707526149508595
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2216	96-97	-0.006012928187175248
2216	98-99	-0.15991650308411565
2216	100	-0.10644711057009815
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1619.0
3	478.0
4	826.0
5	3109.0
6	9813.0
7	17579.0
8	21197.0
9	28617.0
10	18015.0
11	9560.0
12	8402.0
13	10747.0
14	10807.0
15	11571.0
16	12774.0
17	14105.0
18	19192.0
19	31465.0
20	60164.0
21	68154.0
22	29767.0
23	19763.0
24	19961.0
25	21655.0
26	22998.0
27	26132.0
28	31002.0
29	36181.0
30	41065.0
31	50315.0
32	66009.0
33	96906.0
34	143101.0
35	367347.0
36	348939.0
37	468564.0
38	380326.0
39	91725.0
40	680.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.943312644415158	46.4031049043932	23.173027652433817	18.480554798757826
2	13.042081237819497	46.52664646425329	24.332650633720018	16.098621664207194
3	15.330583779105575	28.32642510377608	18.981998407965364	37.36099270915298
4	33.3334350900356	29.84892945589419	20.539183326146766	16.278452127923437
5	14.956280303660519	46.35394446526274	21.199263668272177	17.490511562804564
6	37.00507575468458	27.020646490859825	22.21886806854177	13.755409685913827
7	16.24782201544566	27.072491110617573	41.59876086702675	15.080926006910019
8	35.854012203172516	27.19918492881483	21.927551771902177	15.019251096110478
9	35.05725722440787	28.29193743489161	22.64466941155371	14.006135929146806
10-11	14.14047385552329	48.86315504287137	22.024207919469745	14.972163182135592
12-13	14.022029054082417	28.063385521802008	32.23539759523473	25.67918782888084
14-15	14.37499761507729	38.10251733361825	21.939552702976002	25.58293234832846
16-17	24.961039902618836	27.52196990799922	32.41590762526568	15.101082564116261
18-19	14.594677615687832	27.862384235851685	32.50186023971335	25.041077908747134
20-21	25.098354212536677	38.36590819764713	22.13173931459229	14.403998275223898
22-23	24.56163212662604	28.07420353121196	32.52757924621179	14.83658509595021
24-25	14.602423844648044	38.44098556453982	21.48767662737586	25.468913963436275
26-27	15.47433546023961	32.62075009077017	36.5989354847757	15.305978964214514
28-29	24.537057883028126	28.13661218867219	32.06417922407971	15.262150704219978
30-31	21.520550402002574	29.889683015152848	32.355674017316446	16.234092565528137
32-33	17.29400945574156	31.54783391779839	33.26749674696543	17.890659879494624
34-35	17.3667972968332	31.529594028916712	33.28514517501517	17.818463499234916
36-37	17.988613425016123	31.161113930619738	33.780713034651974	17.06955960971217
38-39	19.008463613711207	30.32608571221424	33.172023521061725	17.493427153012824
40-41	16.66734716461309	32.70265165246525	33.34982427889477	17.280176904026895
42-43	16.370284724328922	31.06965757907048	34.62145191663757	17.938605779963023
44-45	16.86451349484666	32.00686476152681	32.90079103116426	18.22783071246227
46-47	18.082407665332383	30.463285545842027	33.96322639975884	17.491080389066752
48-49	16.859667331901107	30.987377081083554	33.86674196662634	18.286213620388992
50-51	17.526091054441107	32.21904656513892	32.63944166097465	17.615420719445325
52-53	18.003649508304694	31.41255890288851	32.83393630608247	17.749855282724326
54-55	18.000881467433402	32.56341032499819	30.355010054834143	19.080698152734264
56-57	18.282493140962288	33.3764018575686	29.288606174851083	19.052498826618027
58-59	19.844026054803614	33.3897383453597	27.748709279829658	19.017526320007022
60-61	19.212479441966245	34.07585198978872	27.247780113942067	19.46388845430297
62-63	17.774432945709737	34.057924698540596	26.957389769202134	21.21025258654753
64-65	18.800269400869258	34.08796739715259	28.166356944704134	18.945406257274016
66-67	19.12929698761309	33.013899904583994	28.708445814259516	19.148357293543402
68-69	19.589068277475263	33.33593448903508	28.19791424199525	18.87708299149441
70-71	17.053075023944626	35.04622934179949	28.664481441485446	19.23621419277044
72-73	17.168676595250236	33.836745604229236	29.115392929638094	19.879184870882433
74-75	17.690786763643885	34.555393570708866	27.661020855596707	20.09279881005054
76-77	19.16364606296313	33.17603553061822	28.201302519677647	19.459015886741003
78-79	18.626112048550457	33.644539027876675	27.5125981926373	20.216750730935576
80-81	19.144677770726815	34.719070381893026	26.972570816716612	19.163681030663554
82-83	18.70895497897808	34.913825012458986	26.88634426188221	19.490875746680718
84-85	18.855214615301254	35.11973993702196	25.958808606897055	20.06623684077973
86-87	18.447089485247332	35.42048612402945	26.629900663104543	19.50252372761867
88-89	20.543056440626874	33.940254824221654	26.415499326497827	19.101189408653642
90-91	19.11087724011191	35.07394029217424	26.36524527708626	19.44993719062759
92-93	17.996474130266385	35.36508396835875	26.654086994348685	19.984354907026173
94-95	18.523946250318865	35.737749269784224	26.191397906495368	19.546906573401543
96-97	18.520772739657133	34.28433128621788	26.970275486740046	20.22462048738494
98-99	19.907384176320747	34.24826009053997	25.55768970627264	20.28666602686664
100	18.32448686002984	35.68279383201749	25.29735216341109	20.695367144541578
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	250777.0
1	135840.5
2	14909.0
3	7206.5
4	5256.0
5	4641.5
6	4461.5
7	5006.0
8	4880.5
9	4224.0
10	4057.5
11	6247.5
12	43769.0
13	46559.0
14	11108.0
15	8018.0
16	8110.0
17	8654.0
18	9198.5
19	9871.5
20	10852.5
21	12224.5
22	13471.0
23	14827.5
24	16526.5
25	18270.0
26	20152.0
27	22271.5
28	24618.0
29	27260.0
30	30225.0
31	33039.0
32	36179.0
33	40041.5
34	44295.5
35	48205.5
36	51324.5
37	53951.5
38	54842.0
39	54155.0
40	53796.0
41	53098.0
42	52165.5
43	53660.5
44	57269.0
45	60100.5
46	62471.0
47	67308.5
48	71660.0
49	81533.5
50	116919.0
51	124563.5
52	90573.0
53	75932.5
54	78034.0
55	82770.0
56	74755.5
57	58359.0
58	46882.0
59	35919.0
60	28479.0
61	23999.0
62	19410.0
63	14785.0
64	10946.5
65	7659.5
66	5765.0
67	4544.5
68	3550.0
69	2703.5
70	1973.5
71	1448.5
72	1124.0
73	802.5
74	544.0
75	382.5
76	239.5
77	152.5
78	90.0
79	54.5
80	31.5
81	15.5
82	7.5
83	4.5
84	3.0
85	2.0
86	1.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13771497693302756
2	0.005761973265970396
3	0.0032053361214669754
4	0.0
5	0.0027474309612574074
6	0.012592391905763117
7	0.0033579711748701647
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.9079381675398665E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	9.539690837699332E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	1.1447629005239198E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	1.3355567172779065E-4
64-65	0.0
66-67	1.9079381675398665E-5
68-69	0.0
70-71	0.0
72-73	5.533020685865612E-4
74-75	5.533020685865612E-4
76-77	6.486989769635545E-4
78-79	6.105402136127573E-4
80-81	0.0010302866104715278
82-83	0.0012210804272255146
84-85	0.001297397953927109
86-87	9.539690837699331E-5
88-89	0.0
90-91	7.631752670159466E-5
92-93	0.0
94-95	1.9079381675398665E-5
96-97	0.0012210804272255146
98-99	7.440958853405479E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2620630.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.84152723268731
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.87513233247384	39.756808225295124
2	9.491206733859146	8.891652373902568
3	2.646574980522718	3.7190884207051136
4	0.9965044036713385	1.867111526482553
5	0.49569321372419184	1.1609513584860012
6	0.29233979408824096	0.8216185455589525
7	0.20340750598558305	0.6669542761669688
8	0.13635643540215295	0.5109714945793693
9	0.09583233937441032	0.4040039821120717
>10	0.5660025167195701	5.4584908433802735
>50	0.12252844754048117	3.9006351431304993
>100	0.06896699253351352	6.2226735235518795
>500	0.0042381363228694725	1.3489486863980977
>1k	0.004156633701275827	3.5335934939614835
>5k	5.705183511555056E-4	1.654212559620466
>10k+	4.890157295618621E-4	20.082285546668675
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	229432	8.754841393100133	No Hit
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	218918	8.353640155229849	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	29723	1.134192923075749	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18894	0.7209716747499647	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17760	0.6776996371101606	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	11303	0.4313085021540622	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA	7481	0.2854657086273148	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6966	0.26581394550165416	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6564	0.25047412263463364	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6142	0.23437112450059716	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5595	0.21349828094771103	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5500	0.2098731984293853	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5082	0.19392283534875202	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4834	0.18445946203775426	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4426	0.16889068659062897	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3526	0.13454779957491136	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2997	0.11436181376233959	No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA	2879	0.1098590796869455	No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG	2823	0.10772218893930086	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.003014542304712989	0.0	0.0	0.0	0.0
2	0.003014542304712989	0.0	0.0	0.0	0.0
3	0.003014542304712989	0.0	0.0	0.0	0.0
4	0.003014542304712989	0.0	0.0	0.0	0.0
5	0.003014542304712989	0.0	0.0	0.0	0.0
6	0.003014542304712989	0.0	0.0	0.0	0.0
7	0.003014542304712989	0.0	0.0	0.0	0.0
8	0.003014542304712989	0.0	0.0	0.0	0.0
9	0.003052701068063786	0.0	0.0	0.0	0.0
10-11	0.003052701068063786	0.0	0.0	1.9079381675398665E-5	0.0
12-13	0.003052701068063786	0.0	0.0	3.815876335079733E-5	0.0
14-15	0.0031671773581161784	0.0	0.0	3.815876335079733E-5	0.0
16-17	0.0032816536481685703	0.0	0.0	3.815876335079733E-5	0.0
18-19	0.0032816536481685703	0.0	0.0	3.815876335079733E-5	0.0
20-21	0.0033770505565455635	0.0	0.0	3.815876335079733E-5	0.0
22-23	0.0034724474649225567	0.0	0.0	3.815876335079733E-5	0.0
24-25	0.0034915268465979554	0.0	0.0	3.815876335079733E-5	0.0
26-27	0.003625082518325746	0.0	0.0	3.815876335079733E-5	0.0
28-29	0.003796796953404334	0.0	0.0	3.815876335079733E-5	0.0
30-31	0.00400667015183372	0.0	0.0	3.815876335079733E-5	0.0
32-33	0.004407337167017092	0.0	0.0	3.815876335079733E-5	0.0
34-35	0.005036956762305247	0.0	0.0	3.815876335079733E-5	1.9079381675398665E-5
36-37	0.006200799044504566	0.0	0.0	3.815876335079733E-5	3.815876335079733E-5
38-39	0.008471245463877007	0.0	0.0	3.815876335079733E-5	3.815876335079733E-5
40-41	0.013069376447648086	0.0	0.0	3.815876335079733E-5	3.815876335079733E-5
42-43	0.01738131670628818	0.0	0.0	3.815876335079733E-5	3.815876335079733E-5
44-45	0.0214261456214727	0.0	0.0	3.815876335079733E-5	3.815876335079733E-5
46-47	0.02531833948325403	0.0	0.0	3.815876335079733E-5	3.815876335079733E-5
48-49	0.030107264283779092	0.0	0.0	3.815876335079733E-5	5.723814502619599E-5
50-51	0.03668965096179163	0.0	0.0	3.815876335079733E-5	7.631752670159466E-5
52-53	0.04434048301362649	0.0	0.0	3.815876335079733E-5	7.631752670159466E-5
54-55	0.0542426821031584	0.0	0.0	3.815876335079733E-5	7.631752670159466E-5
56-57	0.06582386678012539	0.0	0.0	3.815876335079733E-5	7.631752670159466E-5
58-59	0.0784734968309147	0.0	0.0	7.631752670159466E-5	7.631752670159466E-5
60-61	0.09503440012516075	0.0	0.0	7.631752670159466E-5	7.631752670159466E-5
62-63	0.11615527563982705	0.0	0.0	7.631752670159466E-5	7.631752670159466E-5
64-65	0.1416071707948089	0.0	0.0	7.631752670159466E-5	7.631752670159466E-5
66-67	0.16799395565188524	0.0	0.0	7.631752670159466E-5	7.631752670159466E-5
68-69	0.2038631932016347	0.0	0.0	7.631752670159466E-5	7.631752670159466E-5
70-71	0.25472882474824754	0.0	0.0	7.631752670159466E-5	7.631752670159466E-5
72-73	0.3085708398362226	0.0	0.0	7.631752670159466E-5	7.631752670159466E-5
74-75	0.36037136108492995	0.0	0.0	7.631752670159466E-5	7.631752670159466E-5
76-77	0.4187924277750007	0.0	0.0	1.1447629005239198E-4	7.631752670159466E-5
78-79	0.4847880089902047	0.0	0.0	1.1447629005239198E-4	7.631752670159466E-5
80-81	0.5663523656525339	0.0	0.0	1.1447629005239198E-4	9.539690837699331E-5
82-83	0.668198105035812	0.0	0.0	1.1447629005239198E-4	1.1447629005239198E-4
84-85	0.7892377023845412	0.0	0.0	1.1447629005239198E-4	1.1447629005239198E-4
86-87	0.9216676905934833	0.0	0.0	1.1447629005239198E-4	1.1447629005239198E-4
88	1.0318511197689104	0.0	0.0	1.1447629005239198E-4	1.1447629005239198E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	4440	0.0	85.77033	1
AACGTGT	1650	0.0	80.73726	1
CGTGTGG	1945	0.0	75.163475	3
TCGAGTG	1490	0.0	72.25314	2
ATCGAGT	1505	0.0	69.749306	1
ACGTGTG	2040	0.0	67.98285	2
GTGGAAT	79915	0.0	64.06717	6
TGGAATC	80615	0.0	63.49095	7
GGAATCA	81400	0.0	62.841618	8
GAATCAC	81540	0.0	62.739483	9
ACGAGTG	76165	0.0	61.72372	2
AGTGGAA	80965	0.0	61.51223	5
CGAGTGG	78770	0.0	61.50884	3
GAGTGGA	81560	0.0	61.051952	4
AACGAGT	70575	0.0	61.049778	1
ATGAGTG	2375	0.0	60.769028	2
TGAGTGG	2620	0.0	59.387196	3
AATGAGT	2280	0.0	58.22182	1
GCGAGTG	860	0.0	56.851566	2
AGCGAGT	890	0.0	51.83315	1
>>END_MODULE