FastQCFastQC Report
Thu 26 May 2016
SRR2132751_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132751_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3646261
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2404746.595084663440165No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA707781.9411117306193932No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT195270.5355348945124883No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG130550.35803800111950296No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT82350.225847793122873No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT76520.20985881153323915No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC74150.2033590025508322No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA69430.19041423529473067No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT68390.18756199844169136No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58420.1602189201486125No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT56750.1556388859711359No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA39660.10876895537648018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT46150.086.157011
AACGTGT21400.077.178971
AACGAGT637500.076.92661
ACGAGTG701150.075.778752
GTGGAAT771100.075.385626
CGAGTGG730150.075.2048653
TGGAATC775350.074.934897
GAATCAC779200.074.6711659
GGAATCA781150.074.490788
GAGTGGA763800.073.730684
AGTGGAA772850.072.775
ATCGAGT17850.071.4392241
CGTGTGG26200.068.354093
ACGTGTG25300.068.188352
TCGAGTG19950.065.268182
GCGAGTG12250.047.9665572
TGAGTGG35700.045.819663
AGCGAGT12200.044.355111
ATGAGTG34600.043.7466132
GTGTGGA45550.041.9991874
Produced by FastQC (version 0.11.5)