##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132751_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3646261
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.803153696348122	31.0	25.0	33.0	16.0	34.0
2	28.080905892364807	31.0	25.0	33.0	16.0	34.0
3	29.15479829886012	31.0	28.0	34.0	16.0	34.0
4	34.682754745203376	35.0	35.0	37.0	32.0	37.0
5	33.77814095041469	35.0	35.0	37.0	28.0	37.0
6	35.19786351004495	37.0	35.0	37.0	32.0	37.0
7	35.60305693969795	37.0	35.0	37.0	33.0	37.0
8	35.87985637890431	37.0	35.0	37.0	35.0	37.0
9	37.842141031593734	39.0	38.0	39.0	35.0	39.0
10-11	37.84054706999855	39.0	38.0	39.0	35.0	39.0
12-13	37.81929667130247	39.0	38.0	39.0	35.0	39.0
14-15	39.22637435992651	41.0	39.0	41.0	36.0	41.0
16-17	39.20947046851556	40.5	39.0	41.0	36.0	41.0
18-19	39.105338454926844	40.0	39.0	41.0	35.5	41.0
20-21	39.05019264940168	40.0	39.0	41.0	35.0	41.0
22-23	38.92383732267109	40.0	39.0	41.0	35.0	41.0
24-25	38.81725526505097	40.0	39.0	41.0	35.0	41.0
26-27	38.62800825283763	40.0	38.5	41.0	35.0	41.0
28-29	38.44885198837933	40.0	38.0	41.0	34.5	41.0
30-31	38.32088171965748	40.0	38.0	41.0	34.0	41.0
32-33	38.157830994544824	40.0	38.0	41.0	34.0	41.0
34-35	38.068396776862656	40.0	38.0	41.0	34.0	41.0
36-37	37.917616018162164	40.0	38.0	41.0	33.5	41.0
38-39	37.46684809452752	40.0	36.0	41.0	33.0	41.0
40-41	37.216627526115104	40.0	35.0	41.0	33.0	41.0
42-43	37.059843631599605	40.0	35.0	41.0	33.0	41.0
44-45	36.92536107535911	40.0	35.0	41.0	33.0	41.0
46-47	36.791730213498155	40.0	35.0	41.0	32.0	41.0
48-49	36.62998287835127	39.0	35.0	41.0	32.0	41.0
50-51	36.3511377819635	39.0	35.0	41.0	31.0	41.0
52-53	35.921897801611024	39.0	35.0	41.0	29.5	41.0
54-55	35.106325630556896	39.0	35.0	41.0	25.5	41.0
56-57	34.42512384604393	39.0	35.0	41.0	20.0	41.0
58-59	34.091715732911055	38.5	35.0	40.5	16.5	41.0
60-61	33.842897971373965	38.0	34.5	40.0	13.5	41.0
62-63	33.56535173976849	37.0	34.0	40.0	10.5	41.0
64-65	33.293973196104176	37.0	34.0	40.0	7.5	41.0
66-67	32.99631581502257	36.5	34.0	40.0	7.0	41.0
68-69	32.68410750080699	36.0	34.0	39.0	2.0	41.0
70-71	32.36207103660435	35.0	33.0	39.0	2.0	41.0
72-73	32.046577302063675	35.0	33.0	38.5	2.0	40.0
74-75	31.724763668865176	35.0	33.0	37.5	2.0	40.0
76-77	31.407870829872024	35.0	33.0	37.0	2.0	39.0
78-79	31.083864128212433	35.0	33.0	36.5	2.0	39.0
80-81	30.78423458989908	35.0	33.0	36.0	2.0	39.0
82-83	30.501109629837252	35.0	32.5	36.0	2.0	37.0
84-85	30.227892353290123	35.0	32.0	35.0	2.0	37.0
86-87	30.023580319675414	35.0	32.0	35.0	2.0	36.5
88-89	29.81278246400902	35.0	32.0	35.0	2.0	36.0
90-91	29.62784411209181	35.0	32.0	35.0	2.0	36.0
92-93	29.459596282328665	35.0	32.0	35.0	2.0	36.0
94-95	29.283080393861	35.0	32.0	35.0	2.0	35.5
96-97	29.109477352279498	35.0	31.5	35.0	2.0	35.0
98-99	28.950496549753296	35.0	31.0	35.0	2.0	35.0
100	28.84653704164348	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.23232469407746592
1101	2	2.323349046495185
1101	3	1.2156565149247704
1101	4	0.6769045313212416
1101	5	2.303130538175452
1101	6	1.029349122671583
1101	7	0.5535880037479899
1101	8	0.4008691579520729
1101	9	0.3391031659290036
1101	10-11	0.5130738965764507
1101	12-13	0.41945498486133204
1101	14-15	0.5322715211069067
1101	16-17	0.47510800278816845
1101	18-19	0.3993370437327499
1101	20-21	0.4222765816428691
1101	22-23	0.3889722336464061
1101	24-25	0.35504627167866687
1101	26-27	0.3790962945900489
1101	28-29	0.3951792832417311
1101	30-31	0.38217512626800954
1101	32-33	0.36898543011023577
1101	34-35	0.3889154480093566
1101	36-37	0.44304075310893154
1101	38-39	0.4855545741988081
1101	40-41	0.4749227147376658
1101	42-43	0.547155254834351
1101	44-45	0.5314479532126697
1101	46-47	0.5134448161996303
1101	48-49	0.5034828248903835
1101	50-51	0.5472937010375958
1101	52-53	0.624954369405998
1101	54-55	0.7104117826057603
1101	56-57	0.5934647415006253
1101	58-59	0.5781497814589542
1101	60-61	0.5691458118723958
1101	62-63	0.4736029351401072
1101	64-65	0.43025461525872544
1101	66-67	0.454716066918472
1101	68-69	0.5619480884373047
1101	70-71	0.47348294180376627
1101	72-73	0.5183312543751413
1101	74-75	0.5393033261775955
1101	76-77	0.4410994097379586
1101	78-79	0.5387114567535605
1101	80-81	0.547232685030302
1101	82-83	0.5124858354097022
1101	84-85	0.5634701130887727
1101	86-87	0.5959991138829892
1101	88-89	0.5844167325921212
1101	90-91	0.5564146032752397
1101	92-93	0.5918888014699313
1101	94-95	0.69306530322838
1101	96-97	0.6383557945898737
1101	98-99	0.7158971993355472
1101	100	0.7546450401334965
1102	1	1.6857228096453944
1102	2	3.101536197548345
1102	3	3.2296976990193507
1102	4	1.4562190932913026
1102	5	2.65720562729134
1102	6	1.2495521054119578
1102	7	0.7248894573761646
1102	8	0.5597505344434595
1102	9	0.433446875924119
1102	10-11	0.5123377675234266
1102	12-13	0.4729400214310573
1102	14-15	0.583418145018733
1102	16-17	0.5021507276225421
1102	18-19	0.43543845459560515
1102	20-21	0.4324058301002509
1102	22-23	0.43501934074794235
1102	24-25	0.4449062534041772
1102	26-27	0.45531537229229
1102	28-29	0.45367625126630173
1102	30-31	0.4560518528446167
1102	32-33	0.499710448479469
1102	34-35	0.5174207964205095
1102	36-37	0.45923319555656406
1102	38-39	0.546472971053511
1102	40-41	0.624241799918785
1102	42-43	0.62121610828153
1102	44-45	0.5534873243226244
1102	46-47	0.5671599377741003
1102	48-49	0.5482514436952499
1102	50-51	0.6570855531579767
1102	52-53	0.631122766737235
1102	54-55	0.716677366183518
1102	56-57	0.731131386339861
1102	58-59	0.7006311663823084
1102	60-61	0.5992964222423964
1102	62-63	0.6282402552418347
1102	64-65	0.5870118401465234
1102	66-67	0.6189581044579882
1102	68-69	0.5930353953809089
1102	70-71	0.6161960672899056
1102	72-73	0.5866964807941457
1102	74-75	0.5536040146497854
1102	76-77	0.624925975653106
1102	78-79	0.6900758541040801
1102	80-81	0.6827187782867128
1102	82-83	0.6995574464521219
1102	84-85	0.6636959036219423
1102	86-87	0.6574921766294786
1102	88-89	0.6819910233063204
1102	90-91	0.682956996941936
1102	92-93	0.6809749093253039
1102	94-95	0.6401713591845599
1102	96-97	0.6811064602609989
1102	98-99	0.694287555701564
1102	100	0.6807897718988976
1103	1	1.6062824193549545
1103	2	3.101949581926707
1103	3	3.302581062734152
1103	4	1.4540505557072905
1103	5	2.626993528705647
1103	6	1.2481247186982358
1103	7	0.6928123755310693
1103	8	0.49609785079269386
1103	9	0.4229928215684282
1103	10-11	0.5032129988586931
1103	12-13	0.4853057597114727
1103	14-15	0.6044124641278756
1103	16-17	0.5111539910671965
1103	18-19	0.3846172635250369
1103	20-21	0.3339922172279728
1103	22-23	0.3109446683770827
1103	24-25	0.39433744522562364
1103	26-27	0.5100786085374338
1103	28-29	0.5287024187209965
1103	30-31	0.51240238653034
1103	32-33	0.5622259817024897
1103	34-35	0.6210434187398945
1103	36-37	0.5565992484066342
1103	38-39	0.5542988875634904
1103	40-41	0.5863994751542805
1103	42-43	0.6241664914909251
1103	44-45	0.7244722629002069
1103	46-47	0.7580225136061216
1103	48-49	0.7538465846930222
1103	50-51	0.7820201883859141
1103	52-53	0.766497330312248
1103	54-55	0.7805577627093356
1103	56-57	0.6984056257896327
1103	58-59	0.7049286240885806
1103	60-61	0.7953507169637746
1103	62-63	0.7786847922497131
1103	64-65	0.7831788332151106
1103	66-67	0.8047905284659862
1103	68-69	0.7666786629494311
1103	70-71	0.7179236498663215
1103	72-73	0.738375030365205
1103	74-75	0.7416424525830934
1103	76-77	0.7244836336303564
1103	78-79	0.7362521199749033
1103	80-81	0.7032685792406141
1103	82-83	0.7391569712795878
1103	84-85	0.7565938151057061
1103	86-87	0.6879186161869129
1103	88-89	0.7420313539362553
1103	90-91	0.7554440109789589
1103	92-93	0.7532362573445894
1103	94-95	0.709291535347873
1103	96-97	0.7817768410400205
1103	98-99	0.8040426702323877
1103	100	0.8200481394784873
1104	1	6.136420801248381
1104	2	5.1518806223982025
1104	3	4.188543151604474
1104	4	1.9095910135085177
1104	5	2.899740060665657
1104	6	1.320218731332254
1104	7	0.7511667806560425
1104	8	0.5864970049972129
1104	9	0.5014109917672371
1104	10-11	0.5224344816755817
1104	12-13	0.47543829639139545
1104	14-15	0.5727999753506339
1104	16-17	0.43321333227157055
1104	18-19	0.5011549559496657
1104	20-21	0.4411560137452568
1104	22-23	0.40749372597837663
1104	24-25	0.4082573601731738
1104	26-27	0.47712932789700346
1104	28-29	0.49252612538812457
1104	30-31	0.5795129571178137
1104	32-33	0.6012975969001388
1104	34-35	0.6634407704379299
1104	36-37	0.644308801595507
1104	38-39	0.6522463797141782
1104	40-41	0.6325775653662831
1104	42-43	0.6871770748535084
1104	44-45	0.685108722602763
1104	46-47	0.5323965751071924
1104	48-49	0.6343501723980296
1104	50-51	0.6005552329112689
1104	52-53	0.4674212142692227
1104	54-55	0.6055777055896954
1104	56-57	0.5033277426562677
1104	58-59	0.5618552266222423
1104	60-61	0.5347744724597661
1104	62-63	0.5195854941167468
1104	64-65	0.535907728861865
1104	66-67	0.5441567456254717
1104	68-69	0.49607735067783665
1104	70-71	0.5218699640428355
1104	72-73	0.5190673381349526
1104	74-75	0.51270874379383
1104	76-77	0.4727378329931824
1104	78-79	0.45730225063132934
1104	80-81	0.42513388795779505
1104	82-83	0.43493641426460883
1104	84-85	0.4295781644458607
1104	86-87	0.4425451153296862
1104	88-89	0.4738329835386246
1104	90-91	0.4881178278014211
1104	92-93	0.5006715485929867
1104	94-95	0.5114657126432363
1104	96-97	0.4752102996833649
1104	98-99	0.5493830136229327
1104	100	0.5907103716577282
1105	1	1.6251283270572365
1105	2	3.059219712832487
1105	3	3.231560227118333
1105	4	1.4043774821839747
1105	5	2.5868204240996633
1105	6	1.1849939213310634
1105	7	0.7024814852969286
1105	8	0.508497694017052
1105	9	0.3367482656671541
1105	10-11	0.40576940580495346
1105	12-13	0.3760349256223847
1105	14-15	0.4789647732775819
1105	16-17	0.3950939957303703
1105	18-19	0.36935631450629813
1105	20-21	0.3173109783329835
1105	22-23	0.27884294366238294
1105	24-25	0.30511340320457236
1105	26-27	0.3875025801044387
1105	28-29	0.4040194762558684
1105	30-31	0.43682893224307406
1105	32-33	0.39924706921577524
1105	34-35	0.3869104797223102
1105	36-37	0.4061363280944974
1105	38-39	0.47800845719829255
1105	40-41	0.47601785989023426
1105	42-43	0.4554821092537935
1105	44-45	0.5669636514883791
1105	46-47	0.5788513465645408
1105	48-49	0.5243792032485928
1105	50-51	0.5198467971199889
1105	52-53	0.6290710172366829
1105	54-55	0.6416058936546847
1105	56-57	0.7139211603626592
1105	58-59	0.713689864282216
1105	60-61	0.6857243895760945
1105	62-63	0.7401590297314016
1105	64-65	0.7255210902571108
1105	66-67	0.6689736632469447
1105	68-69	0.6942872410132992
1105	70-71	0.7264326924685776
1105	72-73	0.7102897157316477
1105	74-75	0.7321985159758668
1105	76-77	0.6213704555147928
1105	78-79	0.6932139818322653
1105	80-81	0.6643286160731776
1105	82-83	0.6443741169681338
1105	84-85	0.6545786954118746
1105	86-87	0.7547056849005855
1105	88-89	0.7515814439768107
1105	90-91	0.629224303462923
1105	92-93	0.7102330382661926
1105	94-95	0.7298582769800461
1105	96-97	0.7402020935468698
1105	98-99	0.6935572242530412
1105	100	0.7456837121190389
1106	1	-0.6287063101209611
1106	2	1.944588488114622
1106	3	2.812773684320785
1106	4	1.2408907081068676
1106	5	2.5204569265556245
1106	6	1.1533504259267815
1106	7	0.6138181954890456
1106	8	0.4340413952150399
1106	9	0.2874533243092827
1106	10-11	0.4144960354872964
1106	12-13	0.3693576266287195
1106	14-15	0.45414836728242136
1106	16-17	0.41781570041877814
1106	18-19	0.3634780545148786
1106	20-21	0.37837550803887154
1106	22-23	0.28912071710382037
1106	24-25	0.2786385352217593
1106	26-27	0.3156816798628981
1106	28-29	0.38784319194193984
1106	30-31	0.3786094542871439
1106	32-33	0.38181343782371613
1106	34-35	0.5045785611454434
1106	36-37	0.49809535027771545
1106	38-39	0.5577437653429698
1106	40-41	0.5268888104958265
1106	42-43	0.4660496495398263
1106	44-45	0.5574042313352976
1106	46-47	0.5563669318612767
1106	48-49	0.5030226342263404
1106	50-51	0.6433394031457524
1106	52-53	0.6840961951965454
1106	54-55	0.6737633902359335
1106	56-57	0.6260369002118864
1106	58-59	0.5973601080367104
1106	60-61	0.6563648198131204
1106	62-63	0.6387292056575262
1106	64-65	0.6660228066623048
1106	66-67	0.6330876527488272
1106	68-69	0.5682608086429326
1106	70-71	0.5281382132833556
1106	72-73	0.593004900983864
1106	74-75	0.5846089250863713
1106	76-77	0.5847743039500273
1106	78-79	0.6212195698042038
1106	80-81	0.5949221598634651
1106	82-83	0.586471051083894
1106	84-85	0.5583773869583339
1106	86-87	0.598160450127402
1106	88-89	0.6258362484188993
1106	90-91	0.6174645291012162
1106	92-93	0.6662842023905675
1106	94-95	0.693002881085107
1106	96-97	0.7105945172307955
1106	98-99	0.6936366641500271
1106	100	0.6927460783748742
1107	1	-0.9601720909980109
1107	2	1.772439505390988
1107	3	-0.6521339866218661
1107	4	-0.06865551232854017
1107	5	1.8869788425343756
1107	6	0.7986563930389678
1107	7	0.3806200006195013
1107	8	0.29024404818889593
1107	9	0.2775959112593327
1107	10-11	0.3056235262185183
1107	12-13	0.2653549548469343
1107	14-15	0.3423616655880508
1107	16-17	0.2514320458892243
1107	18-19	0.23558265924780386
1107	20-21	0.19525139497929445
1107	22-23	0.21270054599688848
1107	24-25	0.14542949563053043
1107	26-27	0.2270259050634138
1107	28-29	0.21405468227330715
1107	30-31	0.2787498189714057
1107	32-33	0.27631443951297996
1107	34-35	0.2573468197572879
1107	36-37	0.35616556019619594
1107	38-39	0.3654442286902295
1107	40-41	0.3323078831808388
1107	42-43	0.31197690852201987
1107	44-45	0.4159768208986421
1107	46-47	0.39256309308294135
1107	48-49	0.3390666677281615
1107	50-51	0.368856211898823
1107	52-53	0.40505652425130734
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2214	3	-4.0288497662768705
2214	4	-2.108859146207962
2214	5	-3.9620912732084967
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2214	96-97	-1.3249201571686484
2214	98-99	-1.3221489151050214
2214	100	-1.336918018771243
2215	1	-2.2147761896286866
2215	2	-1.0665077710781254
2215	3	-3.522656545536684
2215	4	-1.6649297434728751
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2215	26-27	-0.4731433174154702
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2215	100	-1.0814886931465573
2216	1	3.031895363178972
2216	2	-1.652418667269007
2216	3	-1.949803419299787
2216	4	-0.7548388281410681
2216	5	-2.499701664083318
2216	6	-1.026747451247374
2216	7	-0.4836529948527968
2216	8	-0.35174411710514875
2216	9	-0.3413396394628663
2216	10-11	-0.20842565494009335
2216	12-13	-0.2109392434840487
2216	14-15	-0.3035701648433644
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2216	18-19	-0.307028436361513
2216	20-21	-0.30698550247713996
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2216	30-31	-0.3673669552443002
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2216	34-35	-0.4105768091143389
2216	36-37	-0.4251296155963402
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2216	54-55	-0.14222075429250935
2216	56-57	-0.027717532019224222
2216	58-59	-0.05646603369858383
2216	60-61	-0.031514018790780085
2216	62-63	-0.02050984985558557
2216	64-65	-0.018628131328945585
2216	66-67	-0.024485768977257294
2216	68-69	0.005708816445881837
2216	70-71	0.014863024063316743
2216	72-73	0.10870540317740307
2216	74-75	-0.004418667593263592
2216	76-77	0.01715257828282546
2216	78-79	0.04691742215133132
2216	80-81	0.06386485966630318
2216	82-83	0.03800836115599182
2216	84-85	0.14023036204334005
2216	86-87	0.07661620335510477
2216	88-89	0.06440064934130874
2216	90-91	0.072728425814379
2216	92-93	0.0401360832102462
2216	94-95	-0.035461528673451426
2216	96-97	0.017595565145022363
2216	98-99	-0.05129362732659004
2216	100	-0.1055108755011851
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1848.0
3	435.0
4	543.0
5	1232.0
6	3182.0
7	5706.0
8	6871.0
9	9245.0
10	7351.0
11	7960.0
12	7150.0
13	9860.0
14	9723.0
15	10930.0
16	12793.0
17	14693.0
18	21196.0
19	37199.0
20	74373.0
21	85213.0
22	38198.0
23	23971.0
24	23244.0
25	24909.0
26	27418.0
27	30476.0
28	35581.0
29	42240.0
30	49945.0
31	62089.0
32	80670.0
33	112137.0
34	164781.0
35	311453.0
36	462067.0
37	766797.0
38	828136.0
39	233614.0
40	1032.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.830582463748772	40.68779703597029	25.290699111384267	20.190921388896673
2	15.366251952116844	39.920192910558896	25.358841176267305	19.354713961056948
3	18.84865891346155	25.486705421721773	18.94986160737304	36.71477405744364
4	32.09583735229047	26.968996459661003	21.4373024860261	19.49786370202243
5	17.803642403358708	38.60972792227667	21.942019064419483	21.644610609945136
6	36.39470578974018	23.461199398539797	23.53635449507021	16.607740316649817
7	19.508472512330837	22.790405425567396	38.972898492215336	18.728223569886435
8	35.24827213411218	23.207965639322033	23.309631427920273	18.234130798645516
9	34.682651625870996	24.940233296519366	23.28936409105108	17.087750986558557
10-11	17.202608370602103	41.860648483473895	23.23305983855791	17.703683307366095
12-13	17.101833906020442	24.74884546114499	31.49694988921528	26.652370743619286
14-15	17.34844269239092	32.9169661743907	23.17745493260082	26.557136200617563
16-17	26.204830098558496	24.242984800045857	31.64665118596831	17.905533915427338
18-19	17.619336630043765	24.420111451155034	31.830126806610938	26.13042511219027
20-21	26.26914255452366	33.231809242399265	23.118120178451296	17.380928024625774
22-23	25.803130933304008	24.733514688059905	31.87808003870266	17.585274339933427
24-25	17.446625460985928	33.129910338289	23.051161175790764	26.372303024934308
26-27	17.900229563960117	28.142860335952406	36.37218185590415	17.58472824418332
28-29	25.60067696744693	24.39245298128686	32.444660434346304	17.56220961691991
30-31	23.624049402936322	25.42237102610044	32.76941776795462	18.184161803008617
32-33	19.950752291182667	27.05965096848525	33.700467410314296	19.28912933001779
34-35	19.61097957606436	26.76002897214434	33.96083001189438	19.668161439896924
36-37	19.696766084490385	26.904875981176335	34.235124693487386	19.163233240845898
38-39	20.170401954221052	26.431185260736957	33.904443483338135	19.493969301703856
40-41	19.45429852662769	27.50014329747651	33.7124111521364	19.333147023759405
42-43	19.55991799199694	26.872471960418288	34.055261743672474	19.5123483039123
44-45	19.659947546267258	27.289955381691	33.470272698525974	19.579824373515777
46-47	20.00254507288425	26.639425976363185	33.77947985621435	19.578549094538214
48-49	19.53782244331933	27.167240633624417	33.585747153042526	19.709189770013722
50-51	19.863484813621405	27.532505215616766	32.961354110416124	19.642655860345705
52-53	20.0742185855548	27.34165849275826	32.687250608946464	19.896872312740477
54-55	20.505676911224953	28.151632590206788	31.02829172130026	20.314398777268
56-57	20.784743604475928	28.7618878626626	29.630297995672827	20.82307053718864
58-59	21.25426567105317	29.392136218444044	28.699015237801135	20.654582872701653
60-61	21.203473914785583	29.37727167638301	28.502622275256762	20.916632133574637
62-63	20.413015036368094	29.71114645021837	28.3313625922675	21.544475921146038
64-65	20.781137170378095	29.604682714704182	28.932802671010112	20.681377443907607
66-67	20.865966652684307	29.04567569980258	29.15950464866676	20.928852998846352
68-69	20.910529992230398	29.352561432108125	29.010073606908556	20.72683496875292
70-71	19.87991260088074	29.835124254681716	29.30205764206128	20.98290550237627
72-73	19.961373956134867	29.46223161941005	29.38864906069793	21.18774536375715
74-75	20.287311857918567	29.55264687224974	28.923931922330006	21.236109347501692
76-77	20.85905445374367	29.03977972934596	29.100691767125138	21.000474049785236
78-79	20.790292478974944	29.571998041821107	28.425901166816818	21.211808312387127
80-81	20.829377192332903	29.904188709982638	28.297570367201192	20.968863730483267
82-83	20.684674589099394	30.20911881962417	28.047254855256604	21.058951736019836
84-85	20.901945522730674	30.290289829077693	27.645316325025	21.162448323166636
86-87	20.74771135355131	30.537125712373776	27.633890616686685	21.081272317388226
88-89	21.532920983988806	29.956042642037968	27.519985541353183	20.991050832620044
90-91	20.984905350936398	30.52971552487083	27.45588012261334	21.029499001579428
92-93	20.657613374358004	30.68430921428828	27.442124960336084	21.215952451017632
94-95	20.723212476129643	30.80243339817605	27.317094040768907	21.1572600849254
96-97	20.520007092277357	30.183029299250908	27.600847946756907	21.696115661714828
98-99	21.08812809676589	30.331271073914838	26.80435357649592	21.77624725282335
100	20.9102146006553	30.776266427444444	26.66185991622651	21.651659055673743
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	76450.0
1	41615.5
2	4974.5
3	2695.0
4	2175.5
5	1944.0
6	1859.5
7	2151.0
8	2161.0
9	1876.5
10	1832.0
11	2995.0
12	22301.5
13	25207.5
14	8999.5
15	8583.0
16	9834.5
17	11439.0
18	13054.0
19	14856.5
20	16907.0
21	19312.5
22	22130.5
23	25635.0
24	29580.5
25	33869.0
26	40218.5
27	46770.5
28	50922.5
29	54059.0
30	57202.0
31	61265.0
32	66332.0
33	72266.0
34	79107.5
35	84827.5
36	90031.5
37	94939.5
38	98327.5
39	100037.5
40	100885.0
41	102099.0
42	102603.5
43	103543.0
44	106006.0
45	108794.0
46	111386.0
47	113996.5
48	114343.5
49	116271.0
50	129665.5
51	130657.5
52	114166.0
53	105815.5
54	103692.5
55	103492.0
56	95168.0
57	80865.5
58	73189.0
59	61561.5
60	47129.0
61	38252.5
62	30696.5
63	23370.0
64	16908.5
65	12295.0
66	9361.0
67	7282.0
68	5638.0
69	4260.0
70	3037.5
71	2174.5
72	1765.0
73	1209.0
74	791.5
75	514.5
76	321.5
77	230.0
78	149.5
79	73.0
80	32.0
81	20.5
82	14.5
83	7.0
84	3.0
85	1.5
86	0.5
87	0.0
88	0.5
89	0.5
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1334791996513689
2	0.006006152603996258
3	0.0030990650422446447
4	0.0
5	0.002660259372546288
6	0.012807640484320787
7	0.0032087664596692337
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.371267717807365E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	8.638986622186398E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	1.2341409460266286E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	4.1138031534220945E-5
64-65	0.0
66-67	1.371267717807365E-5
68-69	0.0
70-71	0.0
72-73	4.525183468764304E-4
74-75	3.839549609860622E-4
76-77	5.347944099448722E-4
78-79	4.388056696983568E-4
80-81	8.501859850405662E-4
82-83	8.913240165747872E-4
84-85	0.001179290237314334
86-87	2.74253543561473E-5
88-89	0.0
90-91	5.48507087122946E-5
92-93	0.0
94-95	5.48507087122946E-5
96-97	0.001138152205780113
98-99	4.799437012325777E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3646261.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.48311251428011
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.55633790722048	57.33461526516847
2	8.958021592269409	11.731982716638568
3	1.8094111249892426	3.554576168467822
4	0.5659578387105854	1.4824272332249624
5	0.2670722302099351	0.8744360450138449
6	0.1572481842909875	0.6178260326755793
7	0.09456165472135038	0.43345340329582854
8	0.06592360451760258	0.3453506249578459
9	0.048976528530449655	0.2886421975486456
>10	0.40467344660929644	6.375986029610465
>50	0.049665203330297204	2.1953185945823974
>100	0.018964618177872053	2.335630428246945
>500	0.0021380182590156984	1.0136021606457182
>1k	5.869069715063997E-4	0.6431129044536279
>5k	2.9345348575319987E-4	1.3341840726235015
>10k+	1.6768770614468563E-4	9.438856122845806
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	240474	6.595084663440165	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	70778	1.9411117306193932	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	19527	0.5355348945124883	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	13055	0.35803800111950296	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8235	0.225847793122873	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7652	0.20985881153323915	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	7415	0.2033590025508322	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	6943	0.19041423529473067	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6839	0.18756199844169136	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5842	0.1602189201486125	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5675	0.1556388859711359	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGAACAAGCGAATAAGTGCA	3966	0.10876895537648018	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.003565296066299149	0.0	0.0	0.0	0.0
2	0.003565296066299149	0.0	0.0	0.0	0.0
3	0.003565296066299149	0.0	0.0	0.0	0.0
4	0.003565296066299149	0.0	0.0	0.0	0.0
5	0.003565296066299149	0.0	0.0	0.0	0.0
6	0.003565296066299149	0.0	0.0	0.0	0.0
7	0.003565296066299149	0.0	0.0	0.0	0.0
8	0.003565296066299149	0.0	0.0	0.0	0.0
9	0.003565296066299149	0.0	0.0	0.0	0.0
10-11	0.003565296066299149	0.0	0.0	0.0	0.0
12-13	0.003565296066299149	0.0	0.0	0.0	0.0
14-15	0.0036064340978333694	0.0	0.0	0.0	0.0
16-17	0.0036612848065456643	0.0	0.0	0.0	0.0
18-19	0.0036887101609018114	0.0	0.0	0.0	0.0
20-21	0.0037435608696141064	0.0	0.0	0.0	0.0
22-23	0.003784698901148327	0.0	0.0	0.0	0.0
24-25	0.003825836932682548	0.0	0.0	0.0	0.0
26-27	0.0038669749642167685	0.0	0.0	0.0	0.0
28-29	0.003949251027285211	0.0	0.0	0.0	0.0
30-31	0.004017814413175579	0.0	0.0	0.0	0.0
32-33	0.0041686538621343895	0.0	0.0	0.0	0.0
34-35	0.004593746854654673	0.0	0.0	0.0	0.0
36-37	0.005059977878709177	0.0	0.0	0.0	0.0
38-39	0.006019865281174331	0.0	0.0	0.0	0.0
40-41	0.007185442841310592	0.0	0.0	0.0	0.0
42-43	0.00894066552010402	0.0	0.0	0.0	0.0
44-45	0.010819302293500109	0.0	0.0	0.0	0.0
46-47	0.012917341901745377	0.0	0.0	0.0	0.0
48-49	0.01526220969919597	0.0	0.0	0.0	0.0
50-51	0.018361274741440614	0.0	0.0	0.0	0.0
52-53	0.02277675679278033	0.0	0.0	0.0	0.0
54-55	0.028124700892229054	0.0	0.0	0.0	0.0
56-57	0.03550212121403268	0.0	0.0	0.0	0.0
58-59	0.04404511909597256	0.0	0.0	0.0	0.0
60-61	0.054987835484075334	0.0	0.0	0.0	0.0
62-63	0.06791889006299878	0.0	0.0	0.0	0.0
64-65	0.08303026031323593	0.0	0.0	0.0	0.0
66-67	0.10151494914927922	0.0	0.0	0.0	0.0
68-69	0.1252104553129905	0.0	0.0	0.0	0.0
70-71	0.15561146061677977	0.0	0.0	0.0	0.0
72-73	0.1933624608880165	0.0	0.0	0.0	0.0
74-75	0.2397935858129739	0.0	0.0	0.0	0.0
76-77	0.29460315649373425	0.0	0.0	0.0	0.0
78-79	0.361013652067145	0.0	0.0	0.0	0.0
80-81	0.44228868970158747	2.7425354356147296E-5	0.0	0.0	1.3712677178073648E-5
82-83	0.5428300387712235	2.7425354356147296E-5	0.0	0.0	2.7425354356147296E-5
84-85	0.6684655870767342	2.7425354356147296E-5	0.0	0.0	2.7425354356147296E-5
86-87	0.818304010601545	2.7425354356147296E-5	0.0	0.0	2.7425354356147296E-5
88	0.9419786460705912	2.7425354356147296E-5	0.0	0.0	2.7425354356147296E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	4615	0.0	86.15701	1
AACGTGT	2140	0.0	77.17897	1
AACGAGT	63750	0.0	76.9266	1
ACGAGTG	70115	0.0	75.77875	2
GTGGAAT	77110	0.0	75.38562	6
CGAGTGG	73015	0.0	75.204865	3
TGGAATC	77535	0.0	74.93489	7
GAATCAC	77920	0.0	74.671165	9
GGAATCA	78115	0.0	74.49078	8
GAGTGGA	76380	0.0	73.73068	4
AGTGGAA	77285	0.0	72.77	5
ATCGAGT	1785	0.0	71.439224	1
CGTGTGG	2620	0.0	68.35409	3
ACGTGTG	2530	0.0	68.18835	2
TCGAGTG	1995	0.0	65.26818	2
GCGAGTG	1225	0.0	47.966557	2
TGAGTGG	3570	0.0	45.81966	3
AGCGAGT	1220	0.0	44.35511	1
ATGAGTG	3460	0.0	43.746613	2
GTGTGGA	4555	0.0	41.999187	4
>>END_MODULE