FastQCFastQC Report
Thu 26 May 2016
SRR2132750_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132750_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2635199
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1884937.152894335494207No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1106444.198696189547734No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG188730.7161887963679403No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT143220.5434883665332296No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83040.3151185166661038No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56460.21425326891821073No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46430.17619162727368975No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42490.16124019476327975No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC30420.11543720227580535No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27670.10500155775711814No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26610.10097909114264235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT38150.042.5264431
ATCGAGT12200.039.5694541
AACGAGT522350.038.7988321
GAGTACT304750.038.5736820
AGTACTT303850.038.3404421
GTACTTT300600.038.15490722
ACGAGTG579200.037.822542
TACTTTT304000.037.5045723
CGAGTGG603900.037.2949643
CATGGGG133650.037.2141625
ACTTTTT306950.036.95063424
GAGTGGA625950.036.7558484
GTGGAAT638300.036.4707266
GCTGAGT638550.036.4571117
AGTGGAA630900.036.382395
CTGAGTA636400.036.30375718
TGGAATC641800.036.2731977
TGAGTAC635150.036.24706319
TGCTGAG644800.036.17537716
CTGCTGA645500.036.15659315