FastQCFastQC Report
Thu 26 May 2016
SRR2132749_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132749_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4312094
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2507695.815480831354789No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA534931.240534181304953No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG239030.5543246506221803No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT196870.4565531270886024No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT62740.14549775584669536No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT58360.13534027783253333No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56970.13211678595132667No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43490.10085587188034398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT55100.043.482721
GAGTACT408000.039.33071520
AGTACTT408500.038.92717421
GTACTTT402200.038.8423322
AACGAGT738000.038.7755051
ATCGAGT18250.038.768671
ACGAGTG813400.038.16412
TACTTTT412100.037.6694523
CGAGTGG848750.037.6092453
ACTTTTT413900.037.44185324
CATGGGG194950.037.0215625
GCTGAGT889700.037.01467517
TGAGTAC876400.037.0016219
GAGTGGA883400.036.9330374
GTGGAAT897550.036.8184976
CTGAGTA888000.036.71148318
CTGCTGA901650.036.67535815
AGTGGAA890450.036.6723185
TGCTGAG901600.036.61639816
TGGAATC903750.036.569087