FastQCFastQC Report
Thu 26 May 2016
SRR2132748_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132748_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4864294
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3673997.5529768554285575No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1015682.0880316855847942No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT282780.5813382168100859No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG230310.473470559139723No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT90030.18508338517367576No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA85410.17558560399515327No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT85400.17556504602723438No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT81690.16793803992932993No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA70260.14444028259805022No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50270.10334490472820929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT66950.043.0314251
AACGAGT892550.040.076811
GAGTACT580100.039.70984620
ACGAGTG982250.039.3573882
AGTACTT582100.039.34667221
GTACTTT571700.039.28135722
ATCGAGT20850.039.2218931
CGAGTGG1018200.038.945033
GAGTGGA1051200.038.4989054
GTGGAAT1070350.038.3166246
GCTGAGT1070550.038.26198217
TGAGTAC1058100.038.20492619
AGTGGAA1061700.038.1004075
TGCTGAG1077550.038.09916716
TGGAATC1076150.038.0898447
CTGAGTA1064900.038.08696718
CTGCTGA1078850.038.05733515
TACTTTT587950.037.99029523
CATGGGG213000.037.87103725
ACTTTTT587550.037.8401924