FastQCFastQC Report
Thu 26 May 2016
SRR2132746_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132746_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4008421
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2441126.0899790715596No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA552601.3785977071769657No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT189890.47372768479159255No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG166540.4154753205813462No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT63640.15876575838715543No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT61010.15220457132621548No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT57190.1426746342262951No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45120.11256302668806496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT47050.042.206021
AACGAGT625400.040.3723831
ACGAGTG691000.039.433482
GAGTACT380100.039.33002520
AGTACTT381000.038.90802821
GTACTTT377400.038.8595122
CGAGTGG720600.038.7748683
GAGTGGA746550.038.3056764
GTGGAAT762550.038.000576
AGTGGAA754800.037.84285
ATCGAGT15600.037.7221031
TGGAATC769000.037.6709067
TGAGTAC758750.037.65410219
GCTGAGT769350.037.6185217
CTGAGTA762250.037.5966418
GAATCAC772950.037.4950039
ACTTTTT388150.037.4715824
TGCTGAG774450.037.44179516
GGAATCA773950.037.43858
TACTTTT389950.037.42271823