FastQCFastQC Report
Thu 26 May 2016
SRR2132745_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132745_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3751861
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2668127.111457487364271No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA605801.61466536206965No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG259360.6912836056559665No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT201490.5370401515407953No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT63730.16986236963469595No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT60300.16072023990227782No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54050.14406184024408153No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA51140.13630568936322535No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50340.13417341420697623No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC43610.11623564945503045No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38400.10234920749995803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT49400.042.628741
GAGTACT405550.039.6647920
ATCGAGT15600.039.4371641
GTACTTT397150.039.25200722
AGTACTT405850.039.234421
AACGAGT683000.038.5422361
ACTTTTT407100.038.07111424
ACGAGTG749250.037.975352
TACTTTT410150.037.86844623
CATGGGG179450.037.76610625
CGAGTGG777450.037.5631833
GCTGAGT807450.037.31876417
TGAGTAC798700.037.27593619
GAGTGGA802350.037.138734
CTGAGTA804400.037.1211818
TGCTGAG814250.037.0557316
CTGCTGA816050.036.9766915
GTGGAAT815250.036.9283646
AGTGGAA809150.036.7445685
TGGAATC819250.036.7392657