FastQCFastQC Report
Thu 26 May 2016
SRR2132744_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132744_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2489217
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1365025.48373243473751No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1035244.158898159541735No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG146780.5896633359004056No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT105020.42189973794972474No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80550.32359573311607626No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39500.1586844377167599No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33780.13570532420435824No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31830.12787153550694857No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC27220.10935165556076468No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24990.10039301515295775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT26350.042.1546441
GAGTACT220950.038.747620
AGTACTT222000.038.22744421
GTACTTT217750.038.1451922
ATCGAGT8900.038.135221
AACGAGT378950.037.5704271
CATGGGG96750.037.05975725
ACGAGTG416850.036.812072
ACTTTTT225600.036.6716324
TACTTTT226600.036.5680423
CGAGTGG432850.036.3837553
TGAGTAC447250.035.82002619
GAGTGGA449950.035.72474
GCTGAGT454800.035.7187317
CTGAGTA450950.035.68222418
GTGGAAT455900.035.681716
CTGCTGA458800.035.5367715
TGCTGAG459000.035.511716
TGGAATC458250.035.4992457
AGTGGAA453400.035.4174775