FastQCFastQC Report
Thu 26 May 2016
SRR2132743_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132743_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4493365
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2299965.118569268243288No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA788041.7537858598177536No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG178670.3976307288635577No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT177740.3955610105121663No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA65850.14654941230013585No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT61650.1372022971648197No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60500.13464296802062597No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT58510.130214215849369No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50830.11312234817336228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT44700.042.2501831
GAGTACT358400.039.22016520
AACGAGT591000.039.1224561
AGTACTT361400.038.6572121
GTACTTT360550.038.38838222
ACGAGTG652200.038.3197252
CGAGTGG679350.037.8297963
ATCGAGT14400.037.6467861
CATGGGG168700.037.30605325
GAGTGGA705500.037.1826364
ACTTTTT370300.037.1399924
TGAGTAC709150.037.0003719
GTGGAAT719500.036.9410066
GCTGAGT720050.036.8282517
CTGAGTA714200.036.82806818
TACTTTT375500.036.73697723
TGCTGAG722850.036.72211516
TGGAATC724750.036.6934367
AGTGGAA716600.036.630025
CTGCTGA725600.036.598115