FastQCFastQC Report
Thu 26 May 2016
SRR2132742_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132742_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3821069
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2448276.407290734608562No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1130812.959407432841438No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG268870.7036512557088083No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT192380.5034716724560587No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA87930.2301188489399171No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT71650.18751297084664004No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT61390.1606618462006313No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT58130.1521302023072601No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC47460.12420607950288257No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGTCAAGTAATTAAGTGCATG43540.11394717028140554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT48050.042.9870641
ATCGAGT16350.038.8299061
GAGTACT378650.038.68435320
AGTACTT380950.038.1678621
GTACTTT379500.037.93113322
AACGAGT670650.037.606221
CATGGGG186100.037.15029525
TACTTTT389100.036.85960423
ACGAGTG741100.036.849572
ACTTTTT389650.036.71160524
CGAGTGG772850.036.3681143
GAGTGGA798300.035.947264
GCTGAGT809200.035.84441817
TGAGTAC799400.035.84081319
CTGAGTA802850.035.7854418
TGCTGAG813300.035.68535216
CTGCTGA816150.035.62542315
GTGGAAT815450.035.607556
TGGAATC818150.035.525367
AGTGGAA811500.035.3395