FastQCFastQC Report
Thu 26 May 2016
SRR2132741_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132741_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4174746
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2010214.81516719819601No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA353190.8460155420233949No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG182550.4372721118841721No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT161010.385676158501619No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT61560.1474580728983272No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50690.12142056067602676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT42650.042.1768461
GAGTACT308500.039.3100620
AGTACTT310700.038.79807721
GTACTTT307850.038.73567622
AACGAGT546150.038.6321031
ATCGAGT14800.038.5781171
ACGAGTG607550.037.7509042
CGAGTGG633400.037.3095133
TACTTTT318350.037.28532823
CATGGGG188000.037.2434325
TGAGTAC657400.036.9645619
GCTGAGT666000.036.93643617
CTGAGTA660400.036.90989318
ACTTTTT321350.036.90986624
GAGTGGA661600.036.6744464
GTGGAAT672500.036.5510986
CTGCTGA677500.036.45882415
TGCTGAG677450.036.41605816
TGGAATC676400.036.3674477
GGAATCA679700.036.2879758
Produced by FastQC (version 0.11.5)