FastQCFastQC Report
Thu 26 May 2016
SRR2132740_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132740_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3467040
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2019685.82537265217592No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660611.9054005722460658No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG198610.5728517698094051No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT159220.4592390050302275No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT57970.16720314735336195No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53220.15350269970926209No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50220.1448497854077253No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46870.13518736443767593No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA39390.11361276477917762No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC36410.10501753657298443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT39150.042.8988271
GAGTACT314700.039.28397420
AGTACTT317350.038.63014621
GTACTTT314400.038.62878822
ATCGAGT14450.038.437481
AACGAGT557800.037.9683651
ACGAGTG611250.037.3968622
ACTTTTT323950.037.3202524
TACTTTT326400.037.10077723
CGAGTGG637950.036.8962753
CATGGGG164800.036.85403425
GCTGAGT667100.036.50003417
CTGAGTA662900.036.49236718
TGAGTAC661350.036.4880919
GAGTGGA660000.036.4702764
GTGGAAT672150.036.2611476
TGCTGAG673400.036.25655716
CTGCTGA674700.036.1899615
AGTGGAA668550.035.982565
TGGAATC678550.035.9402737