FastQCFastQC Report
Thu 26 May 2016
SRR2132739_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132739_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2713770
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1354194.990069165773075No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA486321.7920457518507464No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT109390.40309237702531897No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG82790.30507375348684673No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46290.17057451442089785No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38930.14345357196814762No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT34500.12712941774726672No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33180.12226533567693651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT29000.041.9574281
AACGAGT348500.040.0503121
GAGTACT208100.039.0167120
ACGAGTG389550.038.9966352
GTACTTT207050.038.54523522
AGTACTT209750.038.4161321
CGAGTGG407850.038.2750553
GAGTGGA425850.037.551624
GTGGAAT432200.037.508226
TGAGTAC426400.037.44883319
CTGAGTA428250.037.41033618
GAATCAC434800.037.257269
GCTGAGT434150.037.19581617
TGGAATC435950.037.1842087
GGAATCA436250.037.1182988
AATCACT435850.037.02113710
AGTGGAA432400.037.017725
TACTTTT215150.036.9816123
ACTTTTT215250.036.95421224
TGCTGAG438600.036.9036916