FastQCFastQC Report
Thu 26 May 2016
SRR2132738_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132738_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3423896
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2484397.256032309392575No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA374871.094863862687418No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT195020.5695850574900639No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG84040.24545138053258625No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA70350.20546768943916519No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT61860.1806713755324344No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT56910.16621416071048886No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42950.1254418942631435No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC39420.11513200167294801No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36500.10660370525272965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT50250.042.806081
AACGAGT588650.042.19851
ACGAGTG650700.041.515672
CGAGTGG676000.040.946223
GAGTACT373100.040.5517620
GAGTGGA700450.040.374964
AGTACTT372000.040.35826521
GTACTTT370250.040.22819522
GTGGAAT713000.040.1055266
TGGAATC715100.039.9817547
AGTGGAA706750.039.9372375
TGAGTAC710800.039.81394219
GGAATCA720100.039.7713478
CTGAGTA714450.039.74909218
GAATCAC720500.039.7425849
GCTGAGT722800.039.6429317
AATCACT722650.039.5391110
CTGCTGA726600.039.47798515
TGCTGAG727300.039.41276616
ATCACTG729500.039.22455611