FastQCFastQC Report
Thu 26 May 2016
SRR2132737_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132737_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3676674
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1850115.032020788353822No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA458001.245691078404014No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT146560.3986211450892845No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG93980.25561145752927783No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA54490.14820460013588369No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48920.13305503833083923No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46820.1273433543468907No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42200.1147776495822039No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC39810.10827720923856725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT37250.043.136051
AACGAGT468400.040.671181
ACGAGTG519200.039.790892
GAGTACT292650.039.74724620
CGAGTGG540750.039.2720073
AGTACTT293900.039.25636321
GTACTTT290650.039.16554622
GAGTGGA561300.038.6029364
GTGGAAT571300.038.4912156
TGAGTAC563400.038.4030619
CTGAGTA567850.038.2338218
TGGAATC576050.038.17667
AGTGGAA568050.038.173135
GGAATCA577300.038.1663448
GAATCAC578650.038.109969
GCTGAGT575700.038.10985617
AATCACT581450.037.86590610
CTGCTGA584350.037.72641415
TGCTGAG585100.037.6442216
ATCACTG586700.037.6352911