FastQCFastQC Report
Thu 26 May 2016
SRR2132736_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132736_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2757767
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1773066.429332137196507No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG325461.180157714556741No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA317111.1498795946140483No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT133260.48321703755248363No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT43600.15809892568879097No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41880.15186199559281113No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41630.15095546505560478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT36650.042.9982761
ATCGAGT11850.039.9888651
GAGTACT285350.038.68373520
AGTACTT285250.038.22688321
GTACTTT279200.038.16491722
TACTTTT286750.036.92223723
ACTTTTT286450.036.91483324
CATGGGG130150.036.5922725
AACGAGT597350.036.026151
ACGAGTG649600.035.5597152
TCGAGTG13800.035.5500952
GCTGAGT688900.035.4471717
CTGAGTA684500.035.3375918
TGAGTAC681000.035.2963219
CTGCTGA693600.035.28625515
TGCTGAG693900.035.24563616
CGAGTGG673900.035.1621633
AACGTGT8100.035.1013341
GAGTGGA695100.034.8398364
CATGGGC49350.034.7682725
Produced by FastQC (version 0.11.5)