FastQCFastQC Report
Thu 26 May 2016
SRR2132735_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132735_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3461183
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2056715.942216866314205No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA827372.3904254701355No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT162090.46830809003742363No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG155970.45062627431141317No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64870.1874214683245584No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50930.14714622139309017No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA48760.14087668869285444No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46730.13501164197327908No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42480.12273260327466072No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC36070.10421292373156807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT41550.042.748371
GAGTACT320900.040.26508320
AGTACTT322550.039.8545221
GTACTTT318650.039.79694422
AACGAGT523100.039.5876851
ACGAGTG577600.038.9677622
CGAGTGG600400.038.5452423
ACTTTTT328700.038.41340624
CATGGGG158150.038.34760325
TGAGTAC616050.038.28460719
GCTGAGT626150.038.2467217
CTGAGTA618750.038.224218
TACTTTT331650.038.19111323
TGCTGAG630200.038.06026516
GAGTGGA621400.038.0568584
CTGCTGA632250.037.9646915
GTGGAAT630550.037.9442026
TGGAATC633150.037.77237
ATCGAGT13050.037.6573641
AGTGGAA627700.037.6468625