FastQCFastQC Report
Thu 26 May 2016
SRR2132734_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132734_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3065937
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1980286.458971596611411No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA509031.6602754720661255No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT153880.5019020286457289No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG119670.39032113184321793No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT49260.16066866344611777No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44500.14514323027511655No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40440.13190094904102725No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA36500.11905006528183718No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35280.11507085762036207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT38750.042.436041
AACGAGT482800.040.6751751
GAGTACT307050.040.39087720
GTACTTT303000.040.0450622
ACGAGTG532900.039.982362
AGTACTT308100.039.88196621
CGAGTGG552050.039.5892073
ATCGAGT11700.039.564091
GAGTGGA571950.039.0240334
TGAGTAC573550.039.00086619
GTGGAAT582500.038.8787426
CTGAGTA577650.038.84971618
TACTTTT312200.038.81567823
CATGGGG147450.038.78786525
GCTGAGT585150.038.75776717
AGTGGAA576550.038.6732565
TGGAATC586200.038.6101767
ACTTTTT313550.038.5643724
TGCTGAG589150.038.53942516
CTGCTGA589950.038.51326815