FastQCFastQC Report
Thu 26 May 2016
SRR2132733_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132733_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3566767
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1657614.647373938359304No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA332270.9315719249393077No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT131140.3676719000708485No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG105720.2964028768910333No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT65090.18249019350016416No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42040.11786584321319558No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38660.10838947427740583No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA36340.10188498435698211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT34700.042.5019451
AACGAGT441000.039.7761651
GAGTACT260700.039.08363720
GTACTTT260650.038.5003822
ACGAGTG494650.038.495472
AGTACTT263750.038.3147521
CGAGTGG514750.038.000983
ATCGAGT11150.037.763361
GAGTGGA538100.037.2315644
GTGGAAT545250.037.1815956
TGAGTAC539750.037.1232119
CATGGGG148300.037.05260525
TGGAATC548400.036.972427
GCTGAGT548900.036.9692517
CTGAGTA543700.036.95465518
GAATCAC550900.036.8355529
AGTGGAA544000.036.830265
ACTTTTT271950.036.82782424
GGAATCA550700.036.8180058
TGCTGAG553450.036.67724216