FastQCFastQC Report
Thu 26 May 2016
SRR2132732_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132732_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3130724
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1873955.9856761566973No Hit
AACGAGTGGAATCACTGCTGAGTAACGAGTGGAATCACTGCTGAGTAACG584711.8676510609047619No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA182680.5835072015291032No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT146120.46672910163910963No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG85160.27201375784003956No Hit
CACTCGTTACTCAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCACT65910.21052638303472299No Hit
CTCAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCACTCGTTACTCA61280.1957374715880416No Hit
GTTACTCAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCACTCGTTA58460.18672997044773032No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA57420.18340805513357294No Hit
CCACTCGTTACTCAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCAC55580.17753082034698683No Hit
AGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCACTCGTTACTCAGCA53960.17235629841531863No Hit
TACTCAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCACTCGTTACT52070.16631935616170573No Hit
ACTCAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCACTCGTTACTC50590.16159201513771254No Hit
CAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCACTCGTTACTCAGC47190.1507319073798904No Hit
TCAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCACTCGTTACTCAG46640.14897512524259562No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46600.14884735926897422No Hit
ACGAGTGGAATCACTGCTGAGTAACGAGTGGAATCACTGCTGAGTAACGA44970.14364089584390063No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44570.14236323610768628No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42400.1354319320387233No Hit
TCCACTCGTTACTCAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCA42150.13463339470358932No Hit
CTCGTTACTCAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCACTCG41350.1320780752311606No Hit
ACTCGTTACTCAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCACTC39740.12693549479289773No Hit
ATTCCACTCGTTACTCAGCAGTGATTCCACTCGTTACTCAGCAGTGATTC38790.12390105291938862No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC38720.12367746246555111No Hit
TTACTCAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCACTCGTTAC38320.12239980272933672No Hit
TCGTTACTCAGCAGTGATTCCACTCGTTACTCAGCAGTGATTCCACTCGT37950.12121796747333842No Hit
GTGATTCCACTCGTTACTCAGCAGTGATTCCACTCGTTACTCAGCAGTGA36390.11623509450210238No Hit
TACGAGTGGAATCACTGCTGAGTAACGAGTGGAATCACTGCTGAGTAACG36020.1150532592461041No Hit
CTGCTGAGTAACGAGTGGAATCACTGCTGAGTAACGAGTGGAATCACTGC34330.10965514686059838No Hit
GATTCCACTCGTTACTCAGCAGTGATTCCACTCGTTACTCAGCAGTGATT33690.10761089128265539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT44400.042.2496261
GAGTACT303400.038.5790320
AGTACTT305450.038.11846521
GTACTTT302750.038.05879222
ATCGAGT14000.037.945941
CATGGGG141200.037.85691525
TGAGTAC576300.037.597719
ACTTTTT311800.036.77070624
TCGAGTG15600.036.527352
TACTTTT314900.036.5204923
ACATGGG263800.035.74804724
TACATGG266800.035.6923723
GAGTACA273000.035.59890720
AGTACAT272500.035.52721
GTACATG271450.035.25923522
ATGGGGA56250.033.98480626
CTTTTTT344050.033.29837825
CATGGGC38900.032.96825
ATGGGGG68200.032.57800726
AACGTGT10500.032.5400851
Produced by FastQC (version 0.11.5)