FastQCFastQC Report
Thu 26 May 2016
SRR2132731_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132731_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5034690
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2856505.673636311272392No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA461680.9169978687863601No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT224390.44568781791927603No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG173330.3442714447165565No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70340.13971068725184668No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT64860.12882620379804915No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA64850.12880634160196558No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT63330.1257872877972626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT54750.042.874861
AACGAGT728850.040.8014341
ACGAGTG803650.039.9647752
CGAGTGG838500.039.2951243
GAGTACT448600.039.0359620
GAGTGGA870750.038.6511464
AGTACTT450950.038.58376721
GTACTTT446000.038.5582522
ATCGAGT16750.038.4242441
GTGGAAT887650.038.4183926
AGTGGAA881600.038.1305435
TGGAATC897400.038.0111667
TGAGTAC885350.037.9186619
GCTGAGT897700.037.8478617
GGAATCA903500.037.8007938
CTGAGTA892900.037.74584618
GAATCAC905250.037.741929
CTGCTGA906450.037.6305415
TGCTGAG907200.037.54123716
AATCACT909550.037.512710