FastQCFastQC Report
Thu 26 May 2016
SRR2132730_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132730_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3803064
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1534054.033721231091562No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA439911.156725208936794No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT119340.3137996099986748No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG106780.2807736078067579No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT63660.1673913455045721No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38950.10241741921776758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT31600.041.501481
AACGAGT387200.039.4656751
GAGTACT237600.038.22690220
ACGAGTG438000.037.9167982
AGTACTT240500.037.56472821
GTACTTT238600.037.53196722
CGAGTGG458750.037.1967933
GAGTGGA478150.036.4876484
GTGGAAT488350.036.3381586
TGAGTAC481850.036.19738819
CTGAGTA486950.035.97186718
TGGAATC495250.035.8847127
ACTTTTT248350.035.8547724
TACTTTT248600.035.8541123
GAATCAC495700.035.8516659
GCTGAGT492800.035.84391817
GGAATCA497650.035.7425968
AGTGGAA490250.035.5960585
AATCACT498150.035.57377610
TGCTGAG500300.035.3813316
Produced by FastQC (version 0.11.5)