FastQCFastQC Report
Thu 26 May 2016
SRR2132729_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132729_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2046319
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1122455.485215159513253No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA494352.4158012509291074No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT85310.41689492205272005No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG56800.2775715809705134No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45700.223327838914656No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38860.18990196543158716No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27760.13565822337572978No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24820.12129096196634052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT23600.041.853261
AACGAGT291150.040.6271361
ACGAGTG326650.039.193632
CGAGTGG339800.038.4990233
GAGTACT179700.038.164620
GAGTGGA353750.037.9269564
AGTACTT180000.037.68552821
GTGGAAT359550.037.676126
ATCGAGT6500.037.6506841
GTACTTT177800.037.53328322
AGTGGAA357850.037.4002045
TGGAATC362400.037.3710147
GGAATCA365000.037.140978
GAATCAC367550.036.972169
AATCACT368950.036.74839810
TGAGTAC365300.036.5667519
GCTGAGT370900.036.55430217
CTGAGTA366800.036.5251518
ATCACTG372400.036.4956611
TGCTGAG375250.036.20089316