FastQCFastQC Report
Thu 26 May 2016
SRR2132728_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132728_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3012763
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2072456.878901526605312No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA614252.0388261539324533No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT158430.5258628043427246No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGTGACAAAGCGTAC87500.290431076058754No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG65050.2159147599728223No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60550.20097830463265778No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59920.19888720088503475No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT52150.17309692133101742No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT48180.1599196485086945No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39790.13207145733003228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT41150.043.1218451
AACGAGT527900.042.09431
ACGAGTG588700.040.7984432
CGAGTGG620050.039.761993
ATCGAGT12750.039.591011
GAGTGGA644600.039.1526834
AGTGGAA652450.038.6545
GTGGAAT659550.038.6481556
TGGAATC664700.038.3739177
GGAATCA672450.037.9512868
GAGTACT335750.037.80980720
GAATCAC679550.037.534729
AGTACTT336200.037.45168721
AATCACT682600.037.29933510
GTACTTT334450.037.1872522
ATCACTG688450.037.02650511
GCTGAGT686800.036.83299317
TGAGTAC682450.036.6777519
TCACTGC695950.036.6685812
TGCTGAG692650.036.6616416