FastQCFastQC Report
Thu 26 May 2016
SRR2132727_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132727_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2200787
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1098694.9922595871386015No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA759023.4488571588254566No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT88170.4006294112060822No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG75140.34142331811302046No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60000.2726297456319035No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51570.23432526637062107No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27260.12386478109876148No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25840.1174125437854731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT20550.042.370781
AACGAGT289550.039.5953751
GAGTACT176150.038.0997520
ACGAGTG324800.038.0410582
CGAGTGG336550.037.6321073
GTACTTT175550.037.41549722
AGTACTT179500.037.09458521
ATCGAGT7050.036.8955571
GAGTGGA350700.036.8681684
GTGGAAT357150.036.7682276
AGTGGAA356150.036.303175
TGGAATC362150.036.293747
CATGGGG79900.036.23110225
ACTTTTT179550.036.20299524
GGAATCA366150.035.9753538
GAATCAC365800.035.9616669
TGAGTAC357550.035.74389619
CTGAGTA360250.035.671418
AATCACT368700.035.6609110
GCTGAGT364300.035.61900717