FastQCFastQC Report
Thu 26 May 2016
SRR2132726_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132726_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2382886
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1329705.580208201315548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA573072.404940899396782No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT107420.45079789801106723No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG102870.4317034050307065No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT55770.23404392824499368No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45730.19191014593228548No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT34010.14272608928836714No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31250.1311434957442362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT26750.043.0067941
AACGAGT348150.039.1212271
GAGTACT213550.037.7428320
ACGAGTG391900.037.735262
ATCGAGT7700.037.494791
AGTACTT214300.037.2822621
GTACTTT212450.037.0995522
CGAGTGG409950.036.99453
CATGGGG102800.036.3134325
GAGTGGA429300.036.265554
GTGGAAT438050.036.108646
TGGAATC440450.035.970317
AGTGGAA433200.035.9644475
GCTGAGT442000.035.7289517
GGAATCA443350.035.720148
TGAGTAC437000.035.64443219
GAATCAC445100.035.5796979
CTGAGTA439650.035.54966718
ACTTTTT220050.035.5383124
TACTTTT221000.035.395523