FastQCFastQC Report
Thu 26 May 2016
SRR2132725_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132725_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7987021
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3639624.556918029888741No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1226531.5356539065065686No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT289270.3621750838015826No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG207340.25959616232385013No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT134830.16881137535509172No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA102120.1278574327023805No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA96560.12089613887330457No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT92790.11617598100718653No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT85540.10709875434157491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT71800.042.484171
AACGAGT905850.040.075521
ATCGAGT23000.038.8099141
ACGAGTG1014600.038.7962952
GAGTACT580700.038.5506420
CGAGTGG1059750.038.1817933
AGTACTT583900.038.01915421
GTACTTT575850.037.97764622
GAGTGGA1100700.037.5669864
GTGGAAT1127300.037.1928636
AGTGGAA1116700.037.019925
TGGAATC1136300.036.9353837
TGAGTAC1122450.036.7528119
GAATCAC1145250.036.7382439
GGAATCA1145550.036.6947488
CTGAGTA1130750.036.5939218
GCTGAGT1146600.036.54273617
AATCACT1152000.036.49051710
ACTTTTT601250.036.15375524
ATCACTG1167250.036.0809411