FastQCFastQC Report
Thu 26 May 2016
SRR2132724_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132724_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2240382
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1201365.362299822083912No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390571.7433187733163362No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT92990.41506314548144024No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG67060.2993239545756036No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51370.22929125479494122No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31970.14269887903045106No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30200.1347984406230723No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27710.12368426455845476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT24650.042.828811
AACGAGT314350.039.9018061
ACGAGTG354000.038.4857752
GAGTACT188750.038.23692320
CGAGTGG371600.037.7218323
AGTACTT190650.037.70586421
GTACTTT189850.037.38970622
ATCGAGT8250.037.134561
GAGTGGA387150.037.128894
GTGGAAT394550.036.8944856
TGGAATC397750.036.6347437
AGTGGAA392800.036.5884135
GGAATCA400300.036.478318
GAATCAC401200.036.407449
TGAGTAC396850.036.2727619
AATCACT402100.036.25483310
GCTGAGT402850.036.25124717
ATCACTG404700.036.1127111
CTGCTGA405600.036.05426815
CTGAGTA401150.036.02651218