FastQCFastQC Report
Thu 26 May 2016
SRR2132723_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132723_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2433776
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1558816.40490332717555No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1495206.143539914930544No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG201720.8288355214284306No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT121710.5000871074412765No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA119420.49067786024679344No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53290.21896016724628725No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT39450.16209379992242506No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36500.14997271729197756No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36170.1486167995739953No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT34980.14372727810611988No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27310.11221246326695636No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC26790.1100758656507419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT32300.042.8029061
ATCGAGT8450.038.3591421
GAGTACT249500.037.89221620
AGTACTT251950.037.34040521
GTACTTT251650.036.98283822
CATGGGG116650.036.73316625
AACGAGT470300.036.3123781
ACTTTTT257550.035.9135724
TACTTTT258250.035.85029623
ACGAGTG518800.035.6505782
CGAGTGG538450.035.1196173
GAGTGGA555150.034.745444
GCTGAGT560800.034.7088617
CTGAGTA555650.034.67823418
TGAGTAC553800.034.63122619
CTGCTGA564200.034.62835315
TGCTGAG563900.034.6233716
GTGGAAT563600.034.5601966
TGGAATC567250.034.369197
AGTGGAA560450.034.3501365
Produced by FastQC (version 0.11.5)