FastQCFastQC Report
Thu 26 May 2016
SRR2132722_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132722_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2357613
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1808247.669791437356343No Hit
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1627156.901684033808772No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG173000.7337930355830241No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA138490.5874161705080521No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT124090.526337443846806No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49200.20868564942592358No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46340.1965547356584817No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45580.19333113619580483No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40720.1727170659476343No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36840.15625974237502083No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC34410.1459527072509356No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32220.13666365090453778No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28830.12228470066970279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT31650.043.0437361
ATCGAGT10000.038.5774271
GAGTACT260000.038.432320
AACGAGT463000.037.970381
AGTACTT261050.037.93226621
GTACTTT257400.037.88908822
AACGTGT7350.037.4902081
ACGAGTG509450.037.2076722
CGAGTGG530100.036.6920433
TACTTTT265150.036.68209523
ACTTTTT264100.036.65303824
GAGTGGA549250.036.2354164
CATGGGG121650.036.19753325
GTGGAAT560800.036.010156
AGTGGAA554400.035.8742455
GCTGAGT563550.035.8057917
TGAGTAC553900.035.6671719
TGCTGAG566650.035.64096516
CTGAGTA559550.035.5939818
CTGCTGA568350.035.59240315