FastQCFastQC Report
Thu 26 May 2016
SRR2132721_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132721_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2580852
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1367935.300303930639959No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA471481.8268385788879022No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT105980.410639587237083No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG97890.3792933496380265No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36870.14285979978704708No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33520.1298795901508494No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32210.1248037469796796No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32140.1245325187186247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT26650.042.013121
AACGAGT362250.039.919041
GAGTACT210850.039.53893720
ACGAGTG398700.039.141342
AGTACTT212800.038.87685421
GTACTTT211050.038.76146722
CGAGTGG416750.038.5404363
ATCGAGT9800.038.0083581
GAGTGGA433250.037.967074
CATGGGG111550.037.8017525
TGAGTAC434650.037.79409819
GTGGAAT442400.037.7628976
GCTGAGT440850.037.75155617
CTGAGTA437650.037.66570718
TGGAATC445250.037.5190057
AGTGGAA438900.037.4575425
GAATCAC446950.037.4149489
TGCTGAG447750.037.287716
GGAATCA448600.037.278058
AATCACT447850.037.275910