FastQCFastQC Report
Thu 26 May 2016
SRR2132720_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132720_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2643402
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1406305.320038344527242No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1098074.1540030612067325No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG158680.6002870543337714No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT108340.40985063944114436No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84870.3210635385764254No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT71930.27211146847887685No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35010.13244296554213092No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32690.12366639656019023No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC27970.10581061828658675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT29400.042.2052351
GAGTACT228000.037.0257420
AACGAGT395400.036.8331261
AGTACTT229100.036.62714421
GTACTTT228000.036.24432422
ATCGAGT9750.035.9416281
ACGAGTG447350.035.4555662
ACTTTTT233850.035.2066224
TACTTTT235500.035.05334523
CGAGTGG467350.034.775593
CATGGGG116250.034.51228325
GAGTGGA484950.034.284764
GTGGAAT497150.033.8834046
GCTGAGT498300.033.8385317
TGAGTAC490100.033.7808719
CTGAGTA493850.033.7203318
TGCTGAG502600.033.6409316
TGGAATC501350.033.6301967
AGTGGAA494150.033.6216435
CTGCTGA504150.033.56804315