FastQCFastQC Report
Thu 26 May 2016
SRR2132719_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132719_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3296402
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1235073.7467214253601355No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA644441.9549800054726334No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT93570.2838549424493736No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT76520.23213188197313311No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG63680.19318032206023417No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52030.1578387587436241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT24350.041.8289571
AACGAGT323550.040.1199531
ACGAGTG370250.037.8312342
GAGTACT212100.037.23217420
CGAGTGG386600.037.2094843
GTACTTT211350.036.67737222
GAGTGGA403650.036.476524
AGTACTT216200.036.25139621
GTGGAAT413050.036.1305126
TGGAATC415050.035.9706767
GGAATCA418200.035.7523358
GAATCAC418950.035.703549
AGTGGAA411850.035.7016535
AATCACT421950.035.3975610
TGAGTAC413650.035.31015819
GCTGAGT423250.035.06016517
CTGAGTA418550.035.0491918
ATCACTG429700.034.84616511
TACTTTT222300.034.74209223
TCACTGC432000.034.686112
Produced by FastQC (version 0.11.5)