FastQCFastQC Report
Thu 26 May 2016
SRR2132718_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132718_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2249855
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1441356.4064128577174975No Hit
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1021844.5418038051341085No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110230.4899426851952681No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG99780.4434952474715037No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT80700.3586897822304104No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46280.2057021452493605No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC37650.16734411773203162No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36650.16289938684937474No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34440.15307653159870302No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26210.11649639643443689No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25790.114629609463721No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24170.10742914543381685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT20750.041.436851
GAGTACT161950.038.27580620
AGTACTT162800.037.73817421
GTACTTT160300.037.69549622
AACGAGT279600.037.6352271
TACTTTT164800.036.4793223
ACGAGTG313950.036.3073582
ACTTTTT167300.035.8684624
CGAGTGG328550.035.6641273
CATGGGG87850.035.28040325
GAGTGGA342050.035.003424
TGAGTAC342700.034.9428819
CTGAGTA345000.034.92032618
GTGGAAT348200.034.8883366
GCTGAGT348200.034.84577617
TGGAATC350850.034.664867
ATCGAGT6900.034.5076141
CTGCTGA352850.034.50501315
GGAATCA353250.034.4916158
TGCTGAG353600.034.40694416
Produced by FastQC (version 0.11.5)